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Degradation bacteria for highly effective degrading organophosphorus pesticide and its use

A technology of organophosphorus pesticides and degrading bacteria, applied in the field of degrading bacteria, can solve the problems of slow action and high cost

Inactive Publication Date: 2006-04-05
INST OF ZOOLOGY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Traditional physical methods (wrapping, incineration, and burial) and chemical methods (including oxidation, reduction, and hydrolysis) are costly, may generate new pollutants, and are relatively slow-acting

Method used

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  • Degradation bacteria for highly effective degrading organophosphorus pesticide and its use
  • Degradation bacteria for highly effective degrading organophosphorus pesticide and its use
  • Degradation bacteria for highly effective degrading organophosphorus pesticide and its use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] Example 1: Degradation of methyl parathion using bacterial strain B2

[0061] Utilize gas chromatography to measure the degradation rate of bacterial strain B2 to MP, and measure the production and the degradation amount of intermediate product p-nitrophenol with spectrophotometer, and bacterial strain utilizes MP to be the growth situation of carbon source (see figure 1 ).

[0062] During the cultivation process, the strain B2 could rapidly degrade MP. After 3 hours of cultivation, the concentration of MP dropped from the initial 100mg / L to 0.805mg / L, while the bacterial concentration hardly increased during this period. With the reduction of methyl parathion, the concentration of its degradation intermediate p-nitrophenol increased. After a period of time, the concentration of p-nitrophenol began to decrease, and it was completely degraded after 32 hours.

Embodiment 2

[0063] Embodiment 2: Bacterial strain B2 takes p-nitrophenol (PNP) as carbon source growth and its degradation to PNP:

[0064] Strain B2 was induced and cultivated with 50mg / L PNP first, and then inserted into the inorganic salt with 50mg / L PNP as carbon source to detect its growth and PNP degradation. from figure 2 It can be seen from the figure that the strain B2 can grow with PNP as the carbon source and degrade PNP at the same time. When the concentration of PNP is 50mg / L, the lag period of the growth of the strain is less than 4 hours, and the strain B2 reaches the growth peak after 24 hours of cultivation. With the increase of colonization density, the amount of PNP was decreasing, and within 48 hours, bacterial strain B2 degraded 50mg / L of PNP completely. In the first 8 hours of culture, the concentration of PNP decreased slowly, and then the degradation rate accelerated, which may be due to the induction of enzymes that degrade PNP after a period of culture, thereby...

Embodiment 3

[0065] Example 3: Bacterial strain B2 grows with p-nitrocatechol (4-NC) as carbon source and its degradation effect on 4-NC

[0066] Insert 1ml of B2 seed solution into the inorganic salt medium containing different concentrations of 4-NC, the concentrations are 10, 20, 30, 40, 50 mg / L, respectively, and measure the disappearance of brown 4-NC. After culturing for 3 days, it was found that 10, 20, and 30 mg / L 4-NC culture fluids became colorless, while in 40, 50 mg / L 4-NC culture fluids, the concentration of 4-NC did not change. Therefore, we choose the highest concentration that does not inhibit the metabolism of 4-NC by the strain, that is, 30mg / L as the test concentration, and measure the growth of the strain B2 in the medium with 30mg / L 4-NC as the carbon source and the degradation of the 4-NC by the strain. In the case of the result, see image 3 . It can be seen from the figure that the growth of bacterial strain B2 goes through a period of lag phase (12h), and during ...

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Abstract

The invention provides a strain of degrading bacterium for effectively degrading organic phosphoric pesticide, the bacterium has a name of Ochrobactrum sp. and a docket number of CGMCC No.1221. The bacterium can be used for degrading organic phosphoric pesticide and nitrogen-containing aromatic hydrocarbons substance, thus used for the biological purification of water body, soil or agricultural products contaminated by these substances.

Description

field of invention [0001] The invention belongs to the field of biological technology, and in particular relates to a degrading bacterium capable of efficiently degrading organophosphorus pesticides and its application. Background technique [0002] Organophosphorus pesticides (Organophosphorus pesticides, OPs) are widely used agricultural chemicals with many varieties, high yield and wide application because of their low residue, high efficiency and low cost of use. The use of organophosphorus pesticides has produced huge economic and social benefits in terms of agricultural production increase and disease vector pest control, but due to its high toxicity to non-target organisms and the potential delayed neurotoxicity of some organophosphate pesticides and ecological Negative effects of the environment. Evidence has shown that OPs are mutagenic and teratogenic, and most neurological and immune system diseases in mammals are related to OPs, including mad cow disease, Gulf W...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20
Inventor 邱星辉柏文琴李梅何风琴
Owner INST OF ZOOLOGY CHINESE ACAD OF SCI
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