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Identification and culture methods for Cr(VI) reducing bacteria

A culturing method and technology of bacterial culture medium are applied in the identification of Cr-reducing bacteria and the field of culturing, which can solve the problems of secondary pollution, high cost, and difficulty in promotion.

Inactive Publication Date: 2019-04-09
TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Application Information

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Problems solved by technology

However, due to high cost, low efficiency, and complicated operation, a large amount of toxic sludge will be generated, which requires further complicated treatment and is prone to secondary pollution, so it is difficult to promote

Method used

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  • Identification and culture methods for Cr(VI) reducing bacteria
  • Identification and culture methods for Cr(VI) reducing bacteria
  • Identification and culture methods for Cr(VI) reducing bacteria

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Embodiment Construction

[0027] The present invention will be further described in detail below in conjunction with the accompanying drawings and through specific embodiments. The following embodiments are only descriptive, not restrictive, and cannot limit the protection scope of the present invention.

[0028] The identification of Cr (VI) reducing bacteria and its culture method, its steps are:

[0029] (1) Strain screening

[0030] According to the determination of the growth of each strain at different Cr(VI) concentrations, the strain with the strongest tolerance to Cr(VI) was screened out. A total of 25 chromium-resistant strains were screened out in the experiment, named ZJ-1, ZJ-2... ZJ-24, ZJ-25, of which 5 strains can grow in 200mg / L Cr(VI) solution, 5 strains can Growing in 150mg / L Cr(VI) solution, 8 strains can grow in 100mg / L Cr(VI) solution, 7 strains can grow in 50mg / L Cr(VI) solution, and the removal rate of Cr(VI) reaches There are 8 strains with more than 50% of ZY-8. Not only can...

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Abstract

The invention discloses identification and culture methods for Cr(VI) reducing bacteria. The methods comprise the following steps: screening and separating Cr(VI) reducing bacteria from chromium-containing soil at a drain outlet of a sewer line of an electroplate factory by adopting a microbial separation and purification method; selecting a ZJ-8, the Cr(VI) removal rate of which reaches 73%, growing in a 150 mg / L Cr(VI) solution as an experimental strain; identifying the ZJ-8 by means of a physiological and biochemical method and a 16s rDNA sequencing method; and separately studying influenceof initial Cr(VI) concentration, pH, temperature and the like on bacterial growth and Cr(VI) removal effect. The ZJ-8 screened by the invention has a relatively high Cr(VI) removal ability in a relatively high metabolic activity condition. The ZJ-8 is a ram-negative bacillus which is Ochrobactrum sp through identification. The ZJ-8 has the relatively high Cr(VI) removal ability, and the removal rate of Cr(VI) can reach 89.4% after 50 mg / L Cr(VI) is treated in 72 hours under the conditions that the pH is 7-9, the inoculum size is 6% (volume fraction) and the temperature is 30 DEG C. The bacteria are expected to be applied to repairing chromium polluted water and soil.

Description

technical field [0001] The invention belongs to the technical field of biological treatment of environmental pollution, and in particular relates to the identification and cultivation method of Cr(VI) reducing bacteria. Background technique [0002] Chromium is a highly toxic heavy metal, widely used in electroplating, tanning, textile printing and dyeing, metal and other processing industries. In nature, chromium is found in two valence states, Cr(III) and Cr(VI). Cr(III) is benign and easily adsorbed in soil and water, while Cr(VI), which is more toxic, is not easily adsorbed and is soluble. Industrial wastewater contains chromium and salt ions that are toxic to the sewage microbial flora system. Cr(VI) can be effectively removed by reduction or biosorption and the harm to human health can be significantly reduced, because Cr(VI) can pass through prokaryotic and eukaryotic Nuclear cell membranes cause oxidative cell damage, causing teratogenic, carcinogenic and other les...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12Q1/04C12R1/01
CPCC12N1/20C12Q1/689
Inventor 杨宗政张洁刘爱霖刘莹曹晓乐江鑫刘森孙星黄晔璇
Owner TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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