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Low-temperature cellulose degrading bacterium

A technology of cellulose degradation and low temperature, applied in the field of microorganisms, can solve the problems of adverse effects on the next crops and the inability to quickly and thoroughly decompose straw

Inactive Publication Date: 2019-09-17
SHENYANG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The corn harvesting period in northern my country is generally late autumn, when the temperature is low, and microorganisms at room temperature cannot quickly and thoroughly decompose the straw in cold climate conditions, which is likely to have adverse effects on the next crops

Method used

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  • Low-temperature cellulose degrading bacterium
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Embodiment Construction

[0024] The technical solution of the present invention will be further described below in conjunction with the examples, but it is not limited thereto. Any modification or equivalent replacement of the technical solution of the present invention without departing from the spirit and scope of the technical solution of the present invention should be covered by the present invention within the scope of protection.

[0025] The invention provides a low-temperature cellulose-degrading bacterium, the specific content of which is as follows:

[0026] Medium used:

[0027] Enrichment medium (Hutchinson straw medium): KH 2 PO 4 1.0g, NaCl 0.1g, FeCl 3 ·6H 2 O0.01g, MgSO 4 ·7H 2 O 0.3g, NaNO 3 2.5g, CaCl 2 ·6H 2 O 0.1g, dilute to 1L with distilled water, add about 1.0g corn stalks per 100mL medium as the sole carbon source. The pretreatment method of the straw section is as follows: soak the straw section with a length of about 3 cm in distilled water overnight to remove so...

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Abstract

The invention discloses a low-temperature cellulose degrading bacterium, and belongs to the technical field of microorganisms. The strain MG-1 is ochrobactrum sp., and the preservation number is CGMCC No. 18079. The strain MG-1 can grow well at 4-35 DEG C and secrete filter paper enzyme, endo-beta-1,4-glucanase, exo-beta-1,4-glucanase and other cellulases. After the strain MG-1 is cultured for 30 days at 16 DEG C, the degradation rate of the strain MG-1 to corn stalks reaches 22.30%; the strain MG-1 can be used for strain resource development for decay-promoting bacterial agents for corn stalk field-returning in a low temperature area.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a strain of low-temperature cellulose-degrading bacteria. Background technique [0002] Corn is the dryland crop with the largest planting area in my country. The planting area of ​​corn exceeds 500 million mu, and the output exceeds 190 million tons, second only to rice. my country has a vast territory and various forms of corn cultivation. The most important planting forms are spring and summer maize. The spring sowing corn area in the north is dominated by the three northeastern provinces, Inner Mongolia and Ningxia. The planting area is stable at more than 6.5 million hectares, accounting for 36% of the country, and the total output is more than 27 million tons, accounting for 40% of the country. [0003] Corn stalks are rich in nutrients and available chemical components, and are important production resources for industrial and agricultural production. In addition...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12R1/01
CPCC12R2001/01C12N1/205
Inventor 韩梅韩晓日罗培宇卫季辉何志刚刘艳魏冉彭帅韩亚东
Owner SHENYANG AGRI UNIV
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