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Ochrobactrum and application thereof

A technology of bacillus pallidum and microbial strains, applied in the field of applied microorganisms, can solve the problems of undiscovered public reports, etc., and achieve the effect of improving quality and reducing pollution

Inactive Publication Date: 2012-05-02
YUNNAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

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  • Ochrobactrum and application thereof
  • Ochrobactrum and application thereof
  • Ochrobactrum and application thereof

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Embodiment Construction

[0016] The following are examples of the present invention, but the content of the present invention is not limited thereto.

[0017] The present invention is realized as follows:

[0018] 1. Cultivation method of Palebacterium 4-40 strain:

[0019] 1) Inclined culture of test tube

[0020] Using Luria-Bertani (LB) medium, the formula is (g / L, g / v): 10 g peptone, 10 g sodium chloride, 5 g yeast extract, 20 g agar, distilled water and dilute the volume to 1000 ml. Sterilize at 121°C for 25 minutes and place on a slope. Inoculate Palebacterium 4-40, culture at 28°C for 24 hours to obtain test tube species.

[0021] 2) Liquid expansion culture

[0022] A. Seed culture (LB medium), the formula is (g / L, g / v): 10 g peptone, 10 g sodium chloride, 5 g yeast extract, distilled water to make the volume to 1000 ml. Sterilize at 121°C for 25 minutes. Inoculate 4-40 test tube species of Palebacterium, cultivate at 28°C for 12 hours to obtain liquid species.

[0023] B. Fermentation medium, the for...

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Abstract

The invention relates to ochrobactrum and application thereof and belongs to the technical field of applied microbiology. The ochrobactrum sp.4-40 disclosed by the invention was preserved in CGMCC (China General Microbiological Culture Collection) on 3rd, November, 2011 with the preservation number of CGMCC No.5442. The ochrobactrum sp.4-40 has the capability of metabolizing nicotine and can growat the concentration of the nicotine as high as 4.5g / L. By culturing the ochrobactrum sp.4-40 in a large amount, a biocatalyst using a complete cell of the strain is used for completely degrading pure nicotine and can be used for reducing the content of the nicotine in tobacco leaves simultaneously. The ochrobactrum sp.4-40 disclosed by the invention has the advantages that the nicotine in tobacco and tobacco wastes can be degraded and the harm of cigarettes on a human body and the pollution to the environment are reduced.

Description

Technical field: [0001] The invention relates to a pallor bacillus strain and its application, and belongs to the technical field of applied microorganisms. Background technique: [0002] Nicotine is an alkaloid in common tobacco, commonly known as Nicotine. It is the main alkaloid of many kinds of tobacco. Humans have long maintained the habit of smoking tobacco. However, long-term smoking can lead to dependence on nicotine. Excessive inhalation of nicotine can inhibit the central nervous system, paralyze the heart, and even be fatal. [0003] At present, the nicotine content of flue-cured tobacco in my country is relatively high, especially the upper flue-cured tobacco is more obvious, the nicotine content reaches 3%-4%, the nicotine content of burley tobacco is even as high as 6%, while the nicotine content of flue-cured tobacco in the United States is 1.5%-3.5% In the meantime, the nicotine content of its burley tobacco is about 2.9%. Generally, the nicotine content of high-q...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A24B15/20A62D3/02C12R1/01A62D101/26
Inventor 杨金奎张克勤雷丽萍麻广慧
Owner YUNNAN UNIV
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