Ochrobactrum and application thereof
A technology of bacillus pallidum and microbial strains, applied in the field of applied microorganisms, can solve the problems of undiscovered public reports, etc., and achieve the effect of improving quality and reducing pollution
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[0016] The following are examples of the present invention, but the content of the present invention is not limited thereto.
[0017] The present invention is realized as follows:
[0018] 1. Cultivation method of Palebacterium 4-40 strain:
[0019] 1) Inclined culture of test tube
[0020] Using Luria-Bertani (LB) medium, the formula is (g / L, g / v): 10 g peptone, 10 g sodium chloride, 5 g yeast extract, 20 g agar, distilled water and dilute the volume to 1000 ml. Sterilize at 121°C for 25 minutes and place on a slope. Inoculate Palebacterium 4-40, culture at 28°C for 24 hours to obtain test tube species.
[0021] 2) Liquid expansion culture
[0022] A. Seed culture (LB medium), the formula is (g / L, g / v): 10 g peptone, 10 g sodium chloride, 5 g yeast extract, distilled water to make the volume to 1000 ml. Sterilize at 121°C for 25 minutes. Inoculate 4-40 test tube species of Palebacterium, cultivate at 28°C for 12 hours to obtain liquid species.
[0023] B. Fermentation medium, the for...
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