Supercharge Your Innovation With Domain-Expert AI Agents!

Ochrobactrum and application thereof

A technology of bacillus pallidum and microbial strains, applied in the field of applied microorganisms, can solve the problems of undiscovered public reports, etc., and achieve the effect of improving quality and reducing pollution

Inactive Publication Date: 2012-05-02
YUNNAN UNIV
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But through document retrieval, do not find the public report of identical document with the content of the present invention

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Ochrobactrum and application thereof
  • Ochrobactrum and application thereof
  • Ochrobactrum and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0016] The following are examples of the present invention, but the content of the present invention is not limited thereto.

[0017] The present invention is realized as follows:

[0018] 1. Cultivation method of Palebacterium 4-40 strain:

[0019] 1) Inclined culture of test tube

[0020] Using Luria-Bertani (LB) medium, the formula is (g / L, g / v): 10 g peptone, 10 g sodium chloride, 5 g yeast extract, 20 g agar, distilled water and dilute the volume to 1000 ml. Sterilize at 121°C for 25 minutes and place on a slope. Inoculate Palebacterium 4-40, culture at 28°C for 24 hours to obtain test tube species.

[0021] 2) Liquid expansion culture

[0022] A. Seed culture (LB medium), the formula is (g / L, g / v): 10 g peptone, 10 g sodium chloride, 5 g yeast extract, distilled water to make the volume to 1000 ml. Sterilize at 121°C for 25 minutes. Inoculate 4-40 test tube species of Palebacterium, cultivate at 28°C for 12 hours to obtain liquid species.

[0023] B. Fermentation medium, the for...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to ochrobactrum and application thereof and belongs to the technical field of applied microbiology. The ochrobactrum sp.4-40 disclosed by the invention was preserved in CGMCC (China General Microbiological Culture Collection) on 3rd, November, 2011 with the preservation number of CGMCC No.5442. The ochrobactrum sp.4-40 has the capability of metabolizing nicotine and can growat the concentration of the nicotine as high as 4.5g / L. By culturing the ochrobactrum sp.4-40 in a large amount, a biocatalyst using a complete cell of the strain is used for completely degrading pure nicotine and can be used for reducing the content of the nicotine in tobacco leaves simultaneously. The ochrobactrum sp.4-40 disclosed by the invention has the advantages that the nicotine in tobacco and tobacco wastes can be degraded and the harm of cigarettes on a human body and the pollution to the environment are reduced.

Description

Technical field: [0001] The invention relates to a pallor bacillus strain and its application, and belongs to the technical field of applied microorganisms. Background technique: [0002] Nicotine is an alkaloid in common tobacco, commonly known as Nicotine. It is the main alkaloid of many kinds of tobacco. Humans have long maintained the habit of smoking tobacco. However, long-term smoking can lead to dependence on nicotine. Excessive inhalation of nicotine can inhibit the central nervous system, paralyze the heart, and even be fatal. [0003] At present, the nicotine content of flue-cured tobacco in my country is relatively high, especially the upper flue-cured tobacco is more obvious, the nicotine content reaches 3%-4%, the nicotine content of burley tobacco is even as high as 6%, while the nicotine content of flue-cured tobacco in the United States is 1.5%-3.5% In the meantime, the nicotine content of its burley tobacco is about 2.9%. Generally, the nicotine content of high-q...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A24B15/20A62D3/02C12R1/01A62D101/26
Inventor 杨金奎张克勤雷丽萍麻广慧
Owner YUNNAN UNIV
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com