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Synergistic effect of multiple bacterial strains on degradation of polyphenol pollutants and research method thereof

A synergistic, contaminant technology, applied in the directions of microorganism-based methods, biochemical equipment and methods, and microbial assay/inspection, which can solve problems such as refractory degradation, refractory microbial utilization, and few studies and reports

Inactive Publication Date: 2013-04-24
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

There are literatures that specifically report the biodegradation characteristics of microorganisms on these three forms of cresol, and the research results confirm that m-cresol is relatively difficult to degrade among the three forms of cresol
Among the chlorophenol organic pollutants, there are also three isomer forms of 2, 3 and 4-chlorophenol, among which 4-chlorophenol is the main pollutant, because it is difficult to be used by microorganisms and related research and reports less

Method used

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  • Synergistic effect of multiple bacterial strains on degradation of polyphenol pollutants and research method thereof
  • Synergistic effect of multiple bacterial strains on degradation of polyphenol pollutants and research method thereof
  • Synergistic effect of multiple bacterial strains on degradation of polyphenol pollutants and research method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Degradation of polyphenol pollutants by Pseudomonas aeruginosa GIMT1.074

[0042] Such as figure 1 shown, according to the final OD 600 When the value is 0.1, Pseudomonas aeruginosa GIMT1.074 is inoculated in 50 mL of inorganic salt culture solution containing initial phenol concentration of 200 mg / L, initial 4-chlorophenol concentration of 100 mg / L and initial m-cresol concentration of 100 mg / L. 250mL Erlenmeyer flask. Place in a shaker at 30°C and 200rpm for cultivation. Take out 5 mL of culture medium every 6 hours, measure the concentration of cells in the culture medium and the concentrations of the three remaining substrates. The phenol concentrations at 0h, 6h, 12h, 18h, and 24h were 168.58mg / L, 173.96mg / L, 171.39mg / L, 11.88mg / L, and 0mg / L; the 4-chlorophenol concentrations were 84.03mg / L, respectively. L, 86.02mg / L, 94.01mg / L, 37.57mg / L and 0mg / L; the concentrations of m-cresol were 78.48mg / L, 82.93mg / L, 88.43mg / L, 24.06mg / L and 0mg / L respectively L; cell v...

Embodiment 2

[0044] Degradation of Polyphenol Contaminants by Sphingobacterium CICC 23249

[0045] Such as figure 2 shown, according to the final OD 600 When the value is 0.1, Sphingobacterium CICC 23249 was inoculated in 250 mL of triangular triangle containing 50 mL of inorganic salt culture solution with initial phenol concentration of 200 mg / L, initial 4-chlorophenol concentration of 100 mg / L and initial m-cresol concentration of 100 mg / L. in the bottle. Place in a shaker at 30°C and 200rpm for cultivation. Take out 5 mL of culture medium every 6 hours, measure the concentration of cells in the culture medium and the concentrations of the three remaining substrates. The phenol concentrations at 0h, 6h, 12h, 18h, 24h and 30h were 170.85mg / L, 158.02mg / L, 178.71mg / L, 106.22mg / L, 47.11mg / L and 0mg / L, respectively; 4- Chlorophenol concentrations were 81.86mg / L, 79.65mg / L, 85.44mg / L, 53.84mg / L, 33.46mg / L and 0mg / L; m-cresol concentrations were 78.86mg / L, 77.34mg / L , 85.57mg / L, 56.47mg / ...

Embodiment 3

[0047] Degradation of Polyphenol Pollutants Using Pallidum pallidum BJS2

[0048] Such as image 3 shown, according to the final OD 600 The value is 0.1. The Pallidum pallidus BJS2 was inoculated in a 250mL Erlenmeyer flask containing 50mL inorganic salt culture solution with an initial phenol concentration of 200mg / L, an initial 4-chlorophenol concentration of 100mg / L and an initial m-cresol concentration of 100mg / L. Place in a shaker at 30°C and 200rpm for cultivation. Take out 5 mL of culture medium every 6 hours, measure the concentration of cells in the culture medium and the concentrations of the three remaining substrates. The phenol concentrations at 0h, 6h, 12h, 18h, 24h and 30h were 231.19mg / L, 228.55mg / L, 172.34mg / L, 136.32mg / L, 146.31mg / L and 170.19mg / L, respectively;4 -Chlorophenol concentrations were 104.13mg / L, 103.52mg / L, 81.43mg / L, 67.99mg / L69.06mg / L and 78.76mg / L; m-cresol concentrations were 100.05mg / L, 99.22mg / L L, 77.78mg / L, 62.54mg / L, 65.24mg / L and 78...

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Abstract

The invention relates to a synergistic effect of multiple bacterial strains on degradation of polyphenol pollutants and a research method thereof. According to the invention, a bacterial suspension is prepared from one or a mixture of two or three selected from the group consisting of pseudomonas aeruginosa GIMT1.074, sphingobacterium multivorum CICC 23249 and ochrobactrum sp. BJS2; then the bacterial suspension is inoculated to inorganic a salt medium containing phenol, meta-cresol and 4-chlorophenol with different concentrations for culture; parts of a culture solution are taken out at different time intervals to measure concentrations of phenol, meta-cresol and 4-chlorophenol in the culture solution so as to determine degradation rates at different times, and cell concentrations in the culture solution are measured to investigate growth conditions of different cells. According to research on synergistic degradation effects of the three phenol degrading bacteria, a mixed bacterial strain has superior capability in degradation of mixed phenolic pollutants and has much better degradation efficiency and effects compared to every individual bacterial strain; moreover, biomass of the mixed bacterial strain is increased to two times of the biomass of an original optimal bacterial strain, i.e., the pseudomonas aeruginosa GIMT1.074.

Description

technical field [0001] The invention relates to a synergistic effect and a research method for degrading polyphenol pollutants by using multiple strains of bacteria. Background technique [0002] Phenolic compounds are derivative compounds of oxygen-containing aromatic hydrocarbons, which are likely to cause extremely high pollution to the environment. Among the 129 priority pollutants listed by the U.S. Environmental Protection Agency (EPA), phenolic pollutants are included, and they are also listed in the blacklist of priority control for toxic organic pollutants in my country. Wastewater containing phenolic pollutants comes from a wide range of sources, mainly from many heavy and light industrial factories - gas plants, coking plants, coal refineries in the gas and coking industry, gas generation in metallurgy, machinery, glass, manufacturing, ceramics and other industries Petroleum refineries, shale retort plants, petrochemical plants, wood preservative plants, wood ret...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/02C12R1/385C12R1/01
Inventor 贾晓强周征西闻建平
Owner TIANJIN UNIV
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