Microbial agent for preventing and controlling brown spot of tobaccos and preparation method of microbial agent

A microbial inoculum and a technology for tobacco brown spot disease, applied in the field of biological pesticides, can solve the problems of weak inhibitory effect on the pathogen of tobacco wildfire disease, and achieve the effects of efficient synergistic disease control, large processing capacity, and avoidance of resource waste.

Active Publication Date: 2019-11-05
CHINA TOBACCO CENTRAL SOUTH AGRICULTURAL EXPERIMENTAL STATION +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Tobacco red spot disease only occurs in the mature stage of tobacco leaves. The present invention provides a microbial agent for preventing and treating tobacco red spot disease. It is used when the disease is first seen in the mature stage of tobacco leaves, and has a strong inhibitory effect on the pathogenic bacteria of tobacco red spot disease; Weak inhibitory effect on tobacco wildfire disease pathogen

Method used

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  • Microbial agent for preventing and controlling brown spot of tobaccos and preparation method of microbial agent
  • Microbial agent for preventing and controlling brown spot of tobaccos and preparation method of microbial agent
  • Microbial agent for preventing and controlling brown spot of tobaccos and preparation method of microbial agent

Examples

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Effect test

Embodiment 1

[0041] A kind of microbial inoculant for preventing and treating tobacco red spot, the microbial inoculant is formulated according to the following volume ratio:

[0042] Achromobacter xylosoxidans 25%;

[0043] Enterobacter sp. 5%;

[0044] Ochrobactrum sp. 15%;

[0045] Stenotrophomonas sp. 40%;

[0046] Bacillus subtilis 15%.

Embodiment 2

[0048] A kind of microbial inoculant for preventing and treating tobacco red spot, the microbial inoculant is formulated according to the following volume ratio:

[0049] Achromobacter xylosoxidans 20%;

[0050] Enterobacter sp. 10%;

[0051] Ochrobactrum sp. 10%;

[0052]Stenotrophomonas sp. 50%;

[0053] Bacillus subtilis 10%.

[0054] A kind of microbial inoculant for preventing and treating tobacco red spot, the microbial inoculant is formulated according to the following volume ratio:

[0055] Achromobacter xylosoxidans 15%;

[0056] Enterobacter sp. 15%;

[0057] Ochrobactrum sp. 5%;

[0058] Stenotrophomonas sp. 60%;

[0059] Bacillus subtilis 5%.

Embodiment 4

[0061] A preparation method of a microbial inoculant for preventing and treating tobacco red spot disease, comprising the steps of:

[0062] S1-Strain preparation: prepare Achromobacter xylosoxidans, Enterobacter sp., Ochrobactrum sp., Stenotrophomonassp., Bacillus subtilis ) each;

[0063] S2-Strain activation: Achromobacter xylosoxidans, Enterobacter sp., Ochrobactrum sp., Stenotrophomonassp., Bacillus subtilis ) are respectively activated into the original species through a solid slope;

[0064] S3-expansion culture: Inoculate the activated strains into liquid medium and cultivate them until the initial bacterial concentration is 1×10 6 Individual / mL, then transfer to aerated culture in new liquid medium, transfer inoculum size is 15% (v / v), the temperature of described aerated culture is 28 ℃, and the aeration rate is 0.20m 3 / min, expand the culture for 24h, when the number of microorganisms is 3×10 9 pcs / ml, use storage tanks to collect the bacterial liquid of each s...

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Abstract

The invention discloses a mixed microbial agent for preventing and controlling brown spot of tobaccos and a preparation method of the mixed microbial agent, and belongs to the technical field of biological pesticide. The mixed microbial agent comprises 15-25% of Achromobacter, 5-15% of Enterobacter sp., 5-15% of Ochrobactrum sp., 40-60% of Stenotrophomonas sp. and 5-15% of Bacillus subtilis. The preparation method of the mixed microbial agent comprises the step of preparing the composite microbial agent from the Achromobacter, Enterobacter sp., Ochrobactrum sp., Stenotrophomonas sp. and Bacillus subtilis at a volume ratio, and the application mode of the microbial agent comprises the steps: adopting spray application, and spraying the microbial agent to both sides of leaves. It is proved through experiments that the prepared microbial agent has a high inhibitory effect on pathogenic bacteria of tobacco brown spot, and a good disease prevention effect on tobacco brown spot is achieved;and the microbial agent has low cost, a good effect and environmental protection, and therefore good prospects for biopesticide development are achieved.

Description

technical field [0001] The invention belongs to the field of biological pesticides, in particular to a microbial agent for preventing and treating tobacco red spot disease and a preparation method thereof. Background technique [0002] Tobacco is an important economic crop, and the tobacco industry is an important pillar industry in my country. Hunan Province is an important tobacco production and planting area in China. In recent years, the tobacco planting area has expanded year by year, and the occurrence of tobacco diseases has also shown a tendency to increase. Tobacco red spot disease is a fungal disease. These two diseases occur year after year in Hunan, Shandong, Heilongjiang, Yunnan and other places. The damage is serious, and there is an upward trend in recent years. [0003] Tobacco red spot disease has a high incidence and a wide area of ​​incidence, and seriously affects the yield and quality of flue-cured tobacco. The occurrence of disease was significantly r...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/00A01P1/00C12N1/20C12R1/025C12R1/01C12R1/125
CPCA01N63/00C12N1/20
Inventor 刘天波周志成尹华群秦崇李佳颖滕凯匡传富蔡海林单雪华周向平
Owner CHINA TOBACCO CENTRAL SOUTH AGRICULTURAL EXPERIMENTAL STATION
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