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Quinolone antibiotic degrading bacterium and application thereof

A technology of quinolones and antibiotics, which is applied in the direction of bacteria, microorganisms, microorganisms, etc., can solve the problems of quinolone antibiotics pollution, etc., and achieve the effects of strong adaptability to the environment, prolonging the life of bacteria, and good effects

Active Publication Date: 2017-07-07
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Aims to solve the problem of quinolone antibiotic pollution in the environment

Method used

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  • Quinolone antibiotic degrading bacterium and application thereof
  • Quinolone antibiotic degrading bacterium and application thereof
  • Quinolone antibiotic degrading bacterium and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Embodiment 1: Isolation and identification of quinolone antibiotic degrading bacteria:

[0046] Inorganic salt medium (g L -1 ): K 2 HPO 4 5.8, KH 2 PO 4 4.5, (NH4) 2 SO 4 2.0, MgCl 2 0.16, CaCl 2 is 0.02, Na 2 MoO 4 2H 2 O is 0.0024, KNO 3 0.0012, FeCl 3 0.0018, MnCl 2 2H 2 O is 0.0015, pH 7.0.

[0047] Basal medium (g L -1 ): tryptone 10.0, yeast extract 5.0, NaCl 10.0, pH 7.5; the medium was sterilized at 121°C for 20 minutes before use.

[0048] Domestication of quinolone antibiotic-degrading flora:

[0049] Domestication is divided into three stages (such as figure 1 ), first put 100mL of inorganic salt culture medium in a 250mL Erlenmeyer flask, sterilize at 121°C for 20min, after cooling, add quinolone antibiotics ciprofloxacin stock solution and yeast extract under aseptic conditions to make The final concentrations were 5mg / L and 1.0g / L respectively. At the same time, 5g of collected manure samples (collected in a large livestock and poultry ...

Embodiment 2

[0064] Example 2: Preliminary research on the degradability of quinolone antibiotics ciprofloxacin (CIP) by Ochrobactrum sp.JOB

[0065] Preparation of seed suspension:

[0066] The bacteria were inoculated in the basal medium, cultured in a constant temperature shaker at 30°C and 120r / min for 24 hours, and then inoculated from the basal medium to expand the culture. Take the bacterial liquid after expansion and cultivation for a certain period of time, centrifuge at 6000r / min for 5min, discard the supernatant, wash the bacterial cells with phosphate buffer for 3 times, and prepare OD 600nm For the bacterial suspension of 1.0, follow-up experiments were carried out. Colony forming units (CFU / mL) of the bacterial suspension were quantified for the dilution plate count method.

[0067] Quantitative Analysis of Degradation Ability of Degrading Strains

[0068] The experimental medium is CIP inorganic salt medium, by adding CIP and external carbon source yeast extract to the in...

Embodiment 3

[0073] Prepare OD 600nm 0.8 to 1.0 of the bacterial suspension is added to the water body polluted by quinolone antibiotics, and the proportion of the bacterial suspension is 1% to 5% (v / v). Stir or ventilate continuously to accelerate the removal efficiency of quinolone antibiotics.

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Abstract

The invention discloses a quinolone antibiotic degrading bacterium and application thereof and belongs to the technical field of biological treatment of environmental pollutants. The degrading bacterium is obtained by domestication and purification of livestock manure, and is named as Ochrobactrum sp. JOB, and was preserved in Guangdong Microbiological Culture Collection Center (GDMCC), Guangdong Province Institute of Microbiology on the fifth floor, No.59 Building, No.100 Courtyard, Xianlie Middle Road, Guanzhou City on December 21st, 2016, with a preservation number of GDMCC NO:60132. The degrading bacterium disclosed by the invention is prepared into bacterium suspension or an immobilized microbial agent which can be used for effectively removing quinolone antibiotic pollution in different environmental media such as a water body and soil; compared with a physical absorption method, a chemical degradation method and the like, the quinolone antibiotic degrading bacterium has the advantages of high efficiency, energy conservation, environment friendliness and the like; particularly, a bacterial body is immobilized by using an embedding agent, so that the life of the bacterial body is prolonged; the microbial agent has an excellent action effect and high ability of adaptation to environment.

Description

technical field [0001] The invention belongs to the technical field of biological treatment of environmental pollutants, in particular to a strain of quinolone antibiotic-degrading bacteria and its application. Background technique [0002] Human beings use a lot of antibiotics in animal husbandry and medical treatment, especially my country has become a big country of antibiotic abuse. The residence time of antibiotics in the organism is short, and the metabolic efficiency is low. 60-90% of antibiotics are excreted in the form of drugs or their metabolites with feces and urine. At present, the treatment rate of feces and urine from most farms and hospital wastewater is low, resulting in a large amount of wastewater containing antibiotics, leachate from livestock and poultry manure and garbage compost directly entering the soil in the form of manure or sewage irrigation, resulting in widespread detection of antibiotics in the soil environment. out. Antibiotics continuously...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N11/10C12N11/08C12N11/04C02F3/34B09C1/10C12R1/01C02F101/38
CPCB09C1/10C02F3/34C02F2101/38C12N1/20C12N11/04C12N11/08C12N11/10C12N1/205C12R2001/01
Inventor 莫测辉冯乃宪喻娇李彦文李慧蔡全英喻乐意
Owner JINAN UNIVERSITY
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