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Compound microorganism live bacteria preparation for enhancing activated sludge, preparation method and applications thereof

A technology of compound microorganisms and live bacteria preparations, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of low efficiency and high processing costs, and achieve convenient preparation, low cost, great economic significance and environmental protection meaning effect

Inactive Publication Date: 2013-12-04
SHANGHAI INST OF PHARMA IND +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The technical problem to be solved by the present invention is to overcome the difficulty in adjusting the activated sludge method in the prior art to obtain high-efficiency microorganisms, while other methods of using microbial strains to treat papermaking wastewater depend on specific strains, or need to Special domestication and cultivation, high cost of treatment, low efficiency and other defects, and provide a compound microbial activity that can effectively, quickly, strengthen the performance of activated sludge, efficiently degrade COD of papermaking wastewater, and improve the chroma and water quality of sewage Bacterial preparation and its preparation method and application

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] (1) Amplified culture:

[0039] Fusiformis (Bacillus.fusiformis) CGMCC 1347 and Comamonas sp. (Comamonas sp.) ACCC 11770 were activated on the slant, respectively received liquid medium according to the inoculum size of 5%. The liquid medium formula was: glucose 1%, malt Powder 0.2%, yeast extract 0.2%, sodium acetate 0.5%, the rest is water, pH 7.0, the percentage is weight and volume percentage, fermented at 37°C for 40 hours.

[0040] After the bacillus pallidus (Ochrobactrum sp.) ACCC11802 is activated on the slant, it is connected with the liquid medium according to the inoculum size of 5%. The liquid medium formula is: glucose 1%, malt powder 0.2%, yeast extract 0.2%, sodium acetate 0.5%, and the rest It is water, pH 7.0, percentage is weight volume percentage, cultivated at 37°C for 40 hours.

[0041] Viable bacteria count (conventional viable bacteria dilution counting method, the same below), detect the number of bacteria, and mix according to the percentage o...

Embodiment 2

[0045] (1) Amplified culture:

[0046] Spindle Bacillus (Bacillus fusiformis) CGMCC1347, Comamonas sp. (Comamonas sp.) ACCC 11770, Comamonas sp. (Comamonas sp.) ACCC 11853 After being activated on the slant, they were respectively connected to the liquid medium according to the inoculum size of 5%. The formula of the liquid culture medium is: 1% glucose, 0.2% malt powder, 0.2% yeast extract, 0.5% sodium acetate, the rest is water, pH 7.0, the percentages are weight and volume percentages, and fermented at 30°C for 54 hours.

[0047] After the bacillus pallidus (Ochrobactrum sp.) ACCC10085 is activated on the slant, it can receive the liquid medium according to the inoculum size of 5%. The liquid medium formula is: glucose 1%, malt powder 0.2%, yeast extract 0.2%, sodium acetate 0.5%, The rest is water, pH 7.0, the percentage is weight volume percentage, cultivated at 30°C for 54 hours.

[0048] Viable bacteria count, the number of detected bacteria, mixed according to the per...

Embodiment 3

[0052] (1) Amplified culture:

[0053] Bacillus fusiformis (Bacillus fusiformis) CGMCC1347, Comamonas sp. ACCC11770 (China Agricultural Microorganisms Collection Center), Pallidum sp. (Ochrobactrum sp.) MCCC1A0137 were activated on the slope and added to the liquid fermentation medium according to the inoculum size of 5% : Glucose 1%, malt powder 0.2%, yeast extract 0.2%, sodium acetate 0.5%, the rest is water, pH 7.0, percentage is weight and volume percentage, cultured at 35°C for 48 hours.

[0054] Viable bacteria count, the number of detected bacteria, mixed according to the percentage of the number of bacteria of the following various bacteria in the total number of bacteria: 30% of Bacillus fusiformis, 10% of Comamonas spp., 60% of Pallidal bacillus. The total number of live bacteria is higher than 1.2 billion / ml fermentation broth.

[0055] (2) Add solid auxiliary materials (20% rice flour, 59% rice bran, 10% bran, 10% soybean meal, 1% trace element, percentage is volu...

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Abstract

The invention discloses a compound microorganism live bacteria preparation for enhancing activated sludge, a preparation method and applications thereof. The preparation contains Bacillus fusiformis, Comamonas sp. and Ochrobactrum sp.. The preparation method comprises the following steps: first, Bacillus fusiformis, Comamonas sp. and Ochrobactrum sp. after inclined plane activation are inoculated in liquid mediums respectively, and cultured at the temperature of 30 DEG C-37 DEG C for 40-54 h to obtain a microorganism bacteria liquid; second, the microorganism bacteria liquids obtained from the first step are mixed uniformly, dried at the temperature of 40 DEG C-60 DEG C, and the processes of crushing to 15-50 meshes and sieving are performed. The preparation can enhance activated sludge performances effectively and rapidly in applications as an activated sludge COD-degrading enhancer in the papermaking wastewater treatment process. The preparation can degrade papermaking wastewater COD efficiently and improve wastewater chromaticity and water quality.

Description

technical field [0001] The invention belongs to the field of microbial environmental protection, and in particular relates to a composite microbial live bacteria preparation for strengthening activated sludge and its preparation method and application. Background technique [0002] Papermaking wastewater can be roughly divided into: pulping cooking liquor, washing wastewater, bleaching wastewater and paper machine white water. Alkali pulp cooking waste liquor, also known as "black liquor", is the main source of pollution in pulp mills. The composition of pulping and papermaking wastewater is complex, and its composition not only depends on the method of pulping, but also depends on many factors such as the type of product and the type of raw material. Suspended substances in wastewater mainly come from fibers and fillers lost in various processes of pulping and papermaking; BOD mainly comes from pulping and cooking processes, such as sugars, alcohols, organic acids, etc. ge...

Claims

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Application Information

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IPC IPC(8): C12N1/00C12N1/20C02F3/34C12R1/01C12R1/07C02F103/28
Inventor 郑玉果周斌周纪祥胡海峰
Owner SHANGHAI INST OF PHARMA IND
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