42 results about "CAR bacillus" patented technology

The present invention relates to the isolation of polypeptides derived from the Clostridium botulinum neurotoxin and the use thereof as immunogens for the production of vaccines and antitoxins, as well as research and drug development applications. Clostridium botulinum is responsible for food bone botulism, a severe and often deadly disease. Botulinum neurotoxin binds to neural cells and are translocated into the cytosol. The toxin then prevents neurotransmitter release by cleaving a SNARE protein. A double mutant E224A / E262 full length botulinum neurotoxin Type A holo form was successfully cloned and purified, which lacks the endopeptidase activity involved in the toxic action of the BoNT, and thus leading to its detoxification (DR BoNT / A). This new molecule can be used as an antidote against botulism, and also as a vaccine candidate for botulism. Due to the poor availability and extreme toxicity of native holo-toxin, a nontoxic form of the holo-toxin is highly desired for research and vaccine development. The full length DR BoNT / A protein has been expressed in E. coli as a soluble form.

The present invention discloses a polypeptide with tubercle bacillus immunogenicity, which contains a sequence shown by Seq ID No.2 in the sequence table. The present invention also discloses a kit containing the polypetide as above. When the polypeptide and the kit are adopted to detect the infectivity of tubercle bacillus, the false positive rate is greatly decreased, while the true positive rate can be kept unchanged.

The invention belongs to the technical field of biology and particularly discloses a method for efficiently degrading azoxystrobin by utilizing ochrobactrum anthropic. Ochrobactrum anthropic SH14 is used for degrading the azoxystrobin under the condition that the temperature is 30.2 DEG C, the pH is 7.9 and the inoculum size is 0.2g / L, and the degradation rate of the ochrobactrum anthropic SH14 on 50mg / L of azoxystrobin is above 85%; the method disclosed by the invention provides theoretical practice basis for future large-scale industrial production and application of the ochrobactrum anthropic SH14 and has wide signification.

The invention relates to a composite probiotics feed additive containing lactic acid bacteria. The composite probiotics feed additive is prepared by spraying, freezing, drying and compounding 40-60% of lactobacillus acidophilus, 15-20% of lactobacillus amylophilus, 5-10% of lactobacillus salivarius, 5-10% of streptococcus thermophilus, 5-10% of bacteroides thetaiotaomicron and 5-10% of glucose. All strains are separated from pig intestines chime. With the consideration of each part of an intestinal canal of animal, the lactic acid bacteria which produce lactic acid of the front section of the intestinal canal are combined with the bacteroides thetaiotaomicron which dissolve cellulose and polysaccharide of the rear section of the intestinal canal, the proportion among the bacteria is similar to the composition of corresponding bacteria in the intestinal canal of a healthy animal, so that the respective advantages can be developed well, the health of the animal is improved further, the feed digestibility is improved, and the production performance of the animal is improved.

The present invention is one kind of natural cell growth regulating factor F and its preparation process. The preparation process includes screening Gram-positive coccus or bacillus and Gram-negative coccus or bacillus, freeze preserving coccus or bacillus strain, conventional culture of the coccus or bacillus strain at 4-38 deg.c, once filtering to eliminate harmful bacteria while leaving beneficial thallus segment, extracting natural gene, adding alanine into the natural gene, safety test, preparing into solution or powder, and sealing. The cell growth regulating factor F has the functions of stimulating and strengthening immunity, strengthening TC, repairing and reforming necrotic, degenerative, atrophic, fibrillated and calcified pancreatic histiocyte, regenerating capillary vessel, so that it can repair and reform skin and soft tissue.

The invention relates to a double-antibody enzyme-linked immunosorbent assay for detecting clove pseudomonas spot pathogenic and variant bacteria, which belongs to the field of the immunoassay. Inactivated clove pseudomonas spot pathogenic and variant inactivated bacteria are used for immunizing an 8-month-old BALB / c mouse, and six hybridoma cell strains for producing specific monoclonal antibodies can be obtained by virtue of immunization, cell fusion and screening. The six antibodies are respectively marked by HRP, and every two of the six antibodies are paired by adopting the clove pseudomonas spot pathogenic and variant bacteria as a target. The antibody 1D3 (CGMCCNo.9312 monoclonal cell strain Q) and the antibody 6C6 (CGMCCNo.9313 monoclonal cell strain R) are respectively used as a coating antibody and an ELISA antibody to establish the double-antibody enzyme-linked ELISA method of the clove pseudomonas spot pathogenic and variant bacteria, and LOD is 1.5*10<5>cfu / mL. By adopting the ELISA method to detect the double-antibody enzyme-linked immunosorbent assay for detecting clove pseudomonas spot pathogenic and variant bacteria, the specificity is good, the sensitivity is high, the cost is low, and the high-flux determination of the double-antibody enzyme-linked immunosorbent assay for detecting clove pseudomonas spot pathogenic and variant bacteria can be realized.

The invention discloses an EPSP synthase gene derived from ochrobactrum anthropi and application thereof. The EPSP synthase gene contains 1353 basic groups and 451 coded amino acids, the nucleotide sequence of the EPSP synthase gene is as shown in SEQ ID NO1, and the amino acid sequence of the coded proteins is as shown in SEQ ID NO2. The EPSP synthase gene derived from ochrobactrum anthropi disclosed by the invention is synthesized by a manual method, has high homology with the reported EPSP synthase gene derived from agrobacterium tumfaciens, has higher glyphosate tolerance, and can be usedfor culturing genetically modified crops.