42 results about "CAR bacillus" patented technology

The invention provides application of bacteroides ovatus in treating or preventing rheumatoid arthritis or related diseases thereof, and further provides a pharmaceutical composition, a medicine, a food and feed containing the bacteroides ovatus. Applied to animals, the bacteroides ovatus can be used for effectively developing an anti-inflammatory efficacy, so that the symptom of redness and swelling of joint is effectively improved and the symptom of arthritis is remarkably relieved; and the bacteroides ovatus can be effectively used for treating or preventing rheumatoid arthritis or related diseases thereof.

The invention provides application of bacteroides thetaiotaomicron in treating or preventing rheumatoid arthritis or related diseases thereof, and further provides a pharmaceutical composition, a medicine, a food and feed containing the bacteroides thetaiotaomicron. Applied to animals, the bacteroides thetaiotaomicron can be used for effectively developing an anti-inflammatory efficacy, so that the symptom of redness and swelling of joint is effectively improved and the symptom of arthritis is remarkably relieved; and the bacteroides thetaiotaomicron can be effectively used for treating or preventing rheumatoid arthritis or related diseases thereof.

The invention relates to a composite probiotics feed additive containing lactic acid bacteria. The composite probiotics feed additive is prepared by spraying, freezing, drying and compounding 40-60% of lactobacillus acidophilus, 15-20% of lactobacillus amylophilus, 5-10% of lactobacillus salivarius, 5-10% of streptococcus thermophilus, 5-10% of bacteroides thetaiotaomicron and 5-10% of glucose. All strains are separated from pig intestines chime. With the consideration of each part of an intestinal canal of animal, the lactic acid bacteria which produce lactic acid of the front section of the intestinal canal are combined with the bacteroides thetaiotaomicron which dissolve cellulose and polysaccharide of the rear section of the intestinal canal, the proportion among the bacteria is similar to the composition of corresponding bacteria in the intestinal canal of a healthy animal, so that the respective advantages can be developed well, the health of the animal is improved further, the feed digestibility is improved, and the production performance of the animal is improved.

The invention provides ochrobactrum intermedium with a collection number CGMCC NO.8839 and a microbial agent. The microbial agent contains bacteria and a culture medium, wherein the bacteria comprise the ochrobactrum intermedium with the collection number CGMCC NO.8839. The invention further provides applications of the ochrobactrum intermedium and the microbial agent to degradation of neonicotinoid insecticides. According to the technical scheme, the residue content of the neonicotinoid insecticides in soil can be effectively reduced.

The invention discloses a food-grade nattokinase expression bacterium. The recombinant bacterium is bacillus licheniformis which is transformed so that the genome contains at least one copy nattokinase gene. Preferably, in the genome of the expression bacterium, the negative regulatory factor gene hrcA is inactivated. The production strain provided by the invention accords with the requirements of a food-grade expression system, multiple copy nattokinase genes are tracelessly integrated into the genome of the bacillus licheniformis to be expressed, the expression quantity is obviously improved compared with that in the prior art, through the inactivation of the negative regulatory factor gene hrcA, the enzyme activity and expression quantity are further improved, the enzyme activity achieves 1350FU / mL, and the protein content achieves 1.45g / L. The nattokinase prepared by the recombinant bacterium is high in purity, can be processed into a health product or can be taken as an active ingredient of a medicine for treating cerebral infarction.

Belonging to the molecular biology field, the invention relates to a primer set for respiratory tract infection pathogen detection, a rapid diagnostic kit and a detection method. The primer set can achieve one-time detection of the following 9 respiratory tract infection pathogens: influenza A virus, influenza B virus, respiratory syncytial virus, parainfluenza virus type I, parainfluenza virus type II and parainfluenza virus type III, adenovirus, mycoplasma pneumonia, legionella pneumonia, chlamydia pneumonia and human rhinovirus. The invention adopts solid phase recombinase-polymerase constant temperature gene amplification method to detect respiratory multiple infection pathogens. The primer set adopted by the invention for respiratory tract infection pathogen detection has strong specificity and high amplification efficiency, can effectively and rapidly detect the 9 pathogens simultaneously, and realizes multiplex detection.

The present invention discloses a Proteus mirabilis strain and a method for producing S-equol through daidzein conversion by using the Proteus mirabilis strain, wherein the strain is preserved in the China Center for Type Culture Collection on November 13, 2011, and a preservation number is CCTCC M 2011390. In the prior art, the existing technology is difficult to prepare the S-equol through conversion. With the present invention, the problem in the prior art is solved, and a single function microorganism strain for directly converting a prochiral compound daidzein into a chiral compound S-equol and a preparation method thereof are provided; and compared with the strictly anaerobic equol conversion strains and the mixing bacteria conversion in the previous reports, the Proteus mirabilis LH-52 strain of the present invention is a facultative anaerobe, and is more suitable for industrial production.

The invention discloses a compound microorganism live bacteria preparation for enhancing activated sludge, a preparation method and applications thereof. The preparation contains Bacillus fusiformis, Comamonas sp. and Ochrobactrum sp.. The preparation method comprises the following steps: first, Bacillus fusiformis, Comamonas sp. and Ochrobactrum sp. after inclined plane activation are inoculated in liquid mediums respectively, and cultured at the temperature of 30 DEG C-37 DEG C for 40-54 h to obtain a microorganism bacteria liquid; second, the microorganism bacteria liquids obtained from the first step are mixed uniformly, dried at the temperature of 40 DEG C-60 DEG C, and the processes of crushing to 15-50 meshes and sieving are performed. The preparation can enhance activated sludge performances effectively and rapidly in applications as an activated sludge COD-degrading enhancer in the papermaking wastewater treatment process. The preparation can degrade papermaking wastewater COD efficiently and improve wastewater chromaticity and water quality.

The invention discloses an EPSP synthase gene derived from ochrobactrum anthropi and application thereof. The EPSP synthase gene contains 1353 basic groups and 451 coded amino acids, the nucleotide sequence of the EPSP synthase gene is as shown in SEQ ID NO1, and the amino acid sequence of the coded proteins is as shown in SEQ ID NO2. The EPSP synthase gene derived from ochrobactrum anthropi disclosed by the invention is synthesized by a manual method, has high homology with the reported EPSP synthase gene derived from agrobacterium tumfaciens, has higher glyphosate tolerance, and can be used for culturing genetically modified crops.

The invention belongs to the technical field of biological fermentation and discloses a multi-stage rotating speed regulating policy for improving pseudomonas denitrificans fermentation for productionof vitamin B12. A pseudomonas denitrificans strain is fermented and cultured in a fermenting culture medium. One litter of the culture medium contains 70-85g of saccharose, 40-55g of corn steep liquor, 10-20g of glycine betaine, 0.02-0.05g of auxin, 0.01-0.05g of tryptophan and 0.05-0.1g of glutamine and the like; if the fermentation time is 0-25h, the stirring rotating speed is 150-280 rpm; if the fermentation time is 26-90 h, the stirring rotating speed is 300-400 rpm; if the fermentation time is 91-185h, the stirring rotating speed is 100-200 rpm; vitamin B12 is separated and purified fromfermentation liquor. By controlling the stirring rotating speed and the components of the fermenting culture media, the output of vitamin B12 is improved.

The invention discloses a new bacteria strain-bacteroides uniformis L8 and an application thereof in degrading agar or agar oligosaccharide. The bacteria strain can degrade agar with molecular weight of 100-300kDa into agar oligosaccharide and further degrade the agar oligosaccharide into D-galactose, or degrade the agar oligosaccharide with molecular weight of 0.5-10kDa into D-galactose finally, in addition, the agar oligosaccharide has the specificity to promote growth of the bacteroides uniformis, so as to play a role in regulating associated symptoms caused by obesity.

The present invention discloses a polypeptide with tubercle bacillus immunogenicity, which contains a sequence shown by Seq ID No.2 in the sequence table. The present invention also discloses an ELISPOT method, which utilizes the polypeptide to detect if a biological sample is infected with tubercle bacillus, and an agent box containing the polypeptide. When the polypeptide, the method and the agent box are adopted to detect the infectivity of tubercle bacillus, the false positive rate is greatly decreased, while the true positive rate can be kept unchanged.

The invention relates to the technical field of microorganisms, and discloses a microbial agent, its application, a grain and oil or feed, and a method for removing zearalenone. The microbial agent contains a white-rot fungus and a bacillus, and a dry weight ratio of the white rot fungus to the bacillus is 1:(2-50). The white rot fungus and the bacillus are combined, so the obtained microbial agent can efficiently and safely remove the zearalenone from the grain and oil and / or the feed, and achieves a zearalenone removal rate especially when an appropriate amount of a copper agent is added tothe microbial agent. Additionally, the zearalenone can be completely removed from the grain and oil and / or the feed through combining the spore preparation of the white rot fungus with the spore preparation of the bacillus.

The invention discloses an automatic mycobacterium tuberculosis screening system for mycobacterium tuberculosis sputum smear, comprising a mycobacterium tuberculosis sample scanner, a control recognition system and a remote diagnosis system, wherein the mycobacterium tuberculosis sample scanner comprises an optical system for amplifying a mycobacterium tuberculosis sample, a high-precision slide glass platform for holding the mycobacterium tuberculosis sample, an illumination system and a control PC machine; the control recognition system comprises a mycobacterium tuberculosis sample scanning system, an intelligent mycobacterium tuberculosis recognition system and a mycobacterium tuberculosis image database management system; the control PC machine is in communication connection with the mycobacterium tuberculosis sample scanning system; the mycobacterium tuberculosis image database management system is connected with the intelligent mycobacterium tuberculosis sample recognition system by a mycobacterium tuberculosis sample image display and analyzing application module; the control recognition system is connected with the remote diagnosis system. According to the invention, the mycobacterium tuberculosis sputum smear is successfully digitalized by a fluorescent scanner, automatic scanning is realized, remote share of one mycobacterium tuberculosis sputum smear can be realized, and the mycobacterium tuberculosis sputum smear can be stored for a long time.

The invention discloses bacteroides ovatus for relieving endotoxin infection and an application of the bacteroides ovatus, and belongs to the field of microbial technology. The bacteroides ovatus CCFM1021 provided by the invention has low immunogenicity, and the bacteroides ovatus CCFM1021 is capable of reducing content of proinflammatory factors in blood of mice of endotoxin infection, up-regulating the number of Foxp3+ regulatory T cells and stabilizing intestinal flora composition. The bacteroides ovatus CCFM1021 can be used for preparing a pharmaceutical composition for relieving the endotoxin infection; and the bacteroides ovatus has a broad application prospect.

The invention belongs to the technical field of genetic engineering and microorganisms, and particularly relates to an application of a bacillus licheniformis rex gene to increase of yield of poly gamma-glutamic acid. A molecular biology technique is adopted, a transcription inhibition factor gene rex is knocked out in bacillus licheniformis, a bacillus licheniformis engineering strain WX-02 Deltarex having rex deletion is obtained, and the glycerine metabolism rate of the bacillus licheniformis is notably increased. The glycerine consumption rate of the WX-02 Delta rex in different culture mediums is at least increased by 10.55% than that in an original bacterium namely bacillus licheniformis WX-02, and the highest glycerine consumption rate can reach 27.94%. Through the adoption of thestrain in the poly gamma-glutamic acid fermentation culture mediums, the yield of the poly gamma-glutamic acid can be notably increased, the yield is at least increased by 35.29%, and the yield can beat most increased by 50%, which illustrates that a genetic engineering reformation method has important effects in increasing the microorganism glycerine metabolism rate, and the efficiency of synthesizing biological based chemicals through glycerine can be improved.

The invention belongs to the technical field of biological degradation treatment, and discloses a bacterial strain with a degradation function on chrysene. A chrysene degradation bacterium is identified to be ochrobactrum anthropic DW1, and the bacterium strain preservation number is CGMCC No.8621. The bacterium strain is made into bacterium suspension with the bacterium concentration of 1.25*108 CFU (colony forming unit) / ml, and the degradation and removal rate of chrysene with the original concentration of 0.14mg / L is 56.2% after seven days.

The invention discloses a construction method and application of a tubercle bacillus Pup gene deletion mutation strain. Based on a fusion PCR technique and a T carrier cloning technique, a replacementtype deletion mutation carrier T-Pup-N-K-Pup-C of a gene is constructed and identified for the first time, through an electrotransformation technique, the recombinant Pup gene deletion mutation carrier enters mycobacterium tuberculosis competent cells, and through chromosome homologous recombination, multiple screening and identification, the tubercle bacillus Pup gene deletion mutation strain containing a kanamycin resistant gene is obtained for the first time. According to the tubercle bacillus Pup gene deletion mutation strain constructed by the construction method disclosed by the invention, a Pup gene in an MTB Pup-proteasome system is knocked out, so that the drug tolerance of a clinical drug-tolerance tubercle bacillus separation strain can be reduced, the clinical drug-tolerance tubercle bacillus separation strain can restore sensibility to tubercle resisting medicines, the ubercle bacillus Pup gene deletion mutation strain can help for exploring the pathogenic mechanism of MTB and controlling propagation of tuberculosis, and a new direction is provided for seeking a tubercle resisting drug target.

The present invention discloses a polypeptide with tubercle bacillus immunogenicity, which contains a sequence shown by Seq ID No.2 in the sequence table. The present invention also discloses a kit containing the polypetide as above. When the polypeptide and the kit are adopted to detect the infectivity of tubercle bacillus, the false positive rate is greatly decreased, while the true positive rate can be kept unchanged.

The invention belongs to the technical field of biology and particularly discloses a method for efficiently degrading azoxystrobin by utilizing ochrobactrum anthropic. Ochrobactrum anthropic SH14 is used for degrading the azoxystrobin under the condition that the temperature is 30.2 DEG C, the pH is 7.9 and the inoculum size is 0.2g / L, and the degradation rate of the ochrobactrum anthropic SH14 on 50mg / L of azoxystrobin is above 85%; the method disclosed by the invention provides theoretical practice basis for future large-scale industrial production and application of the ochrobactrum anthropic SH14 and has wide signification.

The invention provides a haemophilus parasuis disease and swine pasteurella multocida disease combined vaccine which comprises at least one haemophilus parasuis antigen and at least one swine pasteurella multocida antigen, preferably, the haemophilus parasuis antigen at least comprises type 4 serotype and type 5 serotype, and the swine pasteurella multocida antigen at least comprises type A capsule serotype, type B capsule serotype and type D capsule serotype. By using the combined vaccine provided by the invention, the haemophilus parasuis antigen and the swine pasteurella multocida antigen do not generate mutual interference or influence, a vaccine composition containing the haemophilus parasuis antigen and the swine pasteurella multocida antigen not only can generate protective immune response to two kinds of bacilli, but also makes the haemophilus parasuis antigen and the swine pasteurella multocida antigen generate mutually-enhanced immune effect. The combined vaccine provided by the invention has an immunization protection stage as long as one year. The invention also correspondingly provides a preparation method of the combined vaccine.

The present invention relates to a method for decomposing dimethylformamide, and more particularly to a microbial flora for decomposing the dimethylformamide and a culture method thereof. The microbial flora for decomposing the dimethylformamide includes: Brevundimonas vesicularis, Brevibacillus parabrevis, achromobacter, Flavobacterium, Stenotrophomonas, Ochrobactrum anthropi, Aquamicrobium defluvii, Sphingobacteria and Nocardioides. The microbial flora utilizes the dimethylformamide as a carbon source and a nitrogen source to oxidatively decompose the dimethylformamide while satisfying the own growth of the microbial flora; the microbial flora can efficiently remove the refractory organic matter dimethylformamide in industrial wastewater, and reduces the operating costs of wastewater treatment.

The invention discloses wheat ochrobactrum of degradable lincomycin. The wheat ochrobactrum is preserved in China General Microbiological Culture Collection Center as the preservation number CGMCC No.10665. The wheat ochrobactrum of the degradable lincomycin can use the lincomycin as an exclusive carbon source to grow, and the degradation capacity of the strain for the lincomycin can reach 100 percent in 24 hours. The strain can be used for harmlessly treating fermented fungi residue in the industrial production of the lincomycin, the treatment method is effective, environment-friendly, capable of saving the resource, low in treatment cost and capable of thoroughly eliminating adverse impact of lincomycin waste hypha on the environment.

The invention relates to a double-antibody enzyme-linked immunosorbent assay for detecting clove pseudomonas spot pathogenic and variant bacteria, which belongs to the field of the immunoassay. Inactivated clove pseudomonas spot pathogenic and variant inactivated bacteria are used for immunizing an 8-month-old BALB / c mouse, and six hybridoma cell strains for producing specific monoclonal antibodies can be obtained by virtue of immunization, cell fusion and screening. The six antibodies are respectively marked by HRP, and every two of the six antibodies are paired by adopting the clove pseudomonas spot pathogenic and variant bacteria as a target. The antibody 1D3 (CGMCCNo.9312 monoclonal cell strain Q) and the antibody 6C6 (CGMCCNo.9313 monoclonal cell strain R) are respectively used as a coating antibody and an ELISA antibody to establish the double-antibody enzyme-linked ELISA method of the clove pseudomonas spot pathogenic and variant bacteria, and LOD is 1.5*10<5>cfu / mL. By adopting the ELISA method to detect the double-antibody enzyme-linked immunosorbent assay for detecting clove pseudomonas spot pathogenic and variant bacteria, the specificity is good, the sensitivity is high, the cost is low, and the high-flux determination of the double-antibody enzyme-linked immunosorbent assay for detecting clove pseudomonas spot pathogenic and variant bacteria can be realized.

Designed to afford a novel microorganism promoting gastric juice secretion and having platelet increasing activity, and to afford a pharmaceutical agent composed of a product secreted by the novel microorganism. The novel microorganism, international deposit number: NITE BP-295, belongs to the species Bacillus pumilus, and is characterized by taking either form of coccus and bacillus, and makes figure-eight movement. The novel microorganism of the present invention is the novel microorganism having the gene represented by SEQ ID No: 1, and an object of the present invention is to afford the microorganism and a gastric juice secretion-promoting composition composed of the product secreted by the novel microorganism.

The invention discloses an HPPD inhibitor resistant gene having high resistance to an HPPD inhibitor and derived from ochrobactrum anthropi and an application thereof in transgenic plants. A method for transforming plants by the gene and high HPPD inhibitor-resisting activity expressed in the transgenic plants play an important role in research and development of plants resisting the HPPD inhibitor.

The invention discloses an EPSP synthase gene derived from ochrobactrum anthropi and application thereof. The EPSP synthase gene contains 1353 basic groups and 451 coded amino acids, the nucleotide sequence of the EPSP synthase gene is as shown in SEQ ID NO1, and the amino acid sequence of the coded proteins is as shown in SEQ ID NO2. The EPSP synthase gene derived from ochrobactrum anthropi disclosed by the invention is synthesized by a manual method, has high homology with the reported EPSP synthase gene derived from agrobacterium tumfaciens, has higher glyphosate tolerance, and can be usedfor culturing genetically modified crops.