Polypeptide for specificity detection of tubercle bacillus infection contamination, method and reagent kit thereof
A Mycobacterium tuberculosis, specific technology, applied in the field of immunology, can solve the problems of high false negatives, easy missed detection of tuberculosis patients, insufficient stimulation intensity of peptides, etc., and achieve the effect of reducing the false positive rate
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Embodiment 1
[0029] 58 healthy volunteers were selected, and all the results were negative after the traditional tuberculin skin test. The lymphocytes in the blood of each healthy volunteer were extracted by conventional methods, and their lymphocytes were divided into two parts for tuberculosis ELISPOT examinations.
[0030] The specific steps are carried out according to the method listed in the literature Selected Pool of Peptides from ESAT-6 and CFP-10 Proteins for Detection of Mycobacterium tuberculosis Infection (PubMed15297485). The stimulating peptide library used in the first part completely adopts the peptide library listed in the literature, that is, it contains the peptide CFP-10 51-70 (Seq ID No. 1). The second part uses the same polypeptide library, but replaces CFP-10 51-70 with the polypeptide DKW51-70 of the present invention (Seq ID No. 2).
[0031]Healthy volunteers should not have a positive reaction, and any positive reaction is a false positive. Results In the reagent gro...
Embodiment 2
[0033] 23 patients who were clinically diagnosed as tuberculosis patients were selected. Use conventional methods to extract the lymphocytes from the patient's blood, and divide their lymphocytes into two parts for tuberculosis ELISPOT examinations.
[0034] The specific steps were carried out according to the method listed in the literature Selected Pool of Peptides from ESAT-6 and CFP-10 Proteins for Detection of Mycobacterium tuberculosis Infection (PubMed15297485). The stimulating peptide library used in the first part completely adopts the peptide library listed in the literature, that is, it contains the peptide CFP-10 51-70 (Seq ID No. 1). The second part uses the same polypeptide library, but replaces CFP-10 51-70 with the polypeptide DKW51-70 of the present invention (Seq ID No. 2).
[0035] As a result, 20 of the two reagent groups had positive reactions, and the sensitivity of diagnosis was exactly the same, both of which were 87%. This shows that for patients with tube...
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