Polypeptide for specificity detection of tubercle bacillus infection contamination, method and reagent kit thereof

A Mycobacterium tuberculosis, specific technology, applied in the field of immunology, can solve the problems of high false negatives, easy missed detection of tuberculosis patients, insufficient stimulation intensity of peptides, etc., and achieve the effect of reducing the false positive rate

Inactive Publication Date: 2009-02-18
SHENZHEN DAKEWE BIOENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But if this polypeptide is removed, the stimulation intensity of the remaining polypeptide is not enough, an

Method used

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  • Polypeptide for specificity detection of tubercle bacillus infection contamination, method and reagent kit thereof
  • Polypeptide for specificity detection of tubercle bacillus infection contamination, method and reagent kit thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] 58 healthy volunteers were selected, and all the results were negative after the traditional tuberculin skin test. The lymphocytes in the blood of each healthy volunteer were extracted by conventional methods, and their lymphocytes were divided into two parts for tuberculosis ELISPOT examinations.

[0030] The specific steps are carried out according to the method listed in the literature Selected Pool of Peptides from ESAT-6 and CFP-10 Proteins for Detection of Mycobacterium tuberculosis Infection (PubMed15297485). The stimulating peptide library used in the first part completely adopts the peptide library listed in the literature, that is, it contains the peptide CFP-10 51-70 (Seq ID No. 1). The second part uses the same polypeptide library, but replaces CFP-10 51-70 with the polypeptide DKW51-70 of the present invention (Seq ID No. 2).

[0031]Healthy volunteers should not have a positive reaction, and any positive reaction is a false positive. Results In the reagent gro...

Embodiment 2

[0033] 23 patients who were clinically diagnosed as tuberculosis patients were selected. Use conventional methods to extract the lymphocytes from the patient's blood, and divide their lymphocytes into two parts for tuberculosis ELISPOT examinations.

[0034] The specific steps were carried out according to the method listed in the literature Selected Pool of Peptides from ESAT-6 and CFP-10 Proteins for Detection of Mycobacterium tuberculosis Infection (PubMed15297485). The stimulating peptide library used in the first part completely adopts the peptide library listed in the literature, that is, it contains the peptide CFP-10 51-70 (Seq ID No. 1). The second part uses the same polypeptide library, but replaces CFP-10 51-70 with the polypeptide DKW51-70 of the present invention (Seq ID No. 2).

[0035] As a result, 20 of the two reagent groups had positive reactions, and the sensitivity of diagnosis was exactly the same, both of which were 87%. This shows that for patients with tube...

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Abstract

The present invention discloses a polypeptide with tubercle bacillus immunogenicity, which contains a sequence shown by Seq ID No.2 in the sequence table. The present invention also discloses an ELISPOT method, which utilizes the polypeptide to detect if a biological sample is infected with tubercle bacillus, and an agent box containing the polypeptide. When the polypeptide, the method and the agent box are adopted to detect the infectivity of tubercle bacillus, the false positive rate is greatly decreased, while the true positive rate can be kept unchanged.

Description

Technical field [0001] The present invention relates to the field of immunology, in particular to polypeptides, methods and kits capable of specifically detecting tuberculosis infection. Background technique [0002] Tuberculosis (including tuberculosis) is an infectious disease that seriously threatens human health. The methods for diagnosing tuberculosis include traditional methods such as sputum smear examination, pathogen culture, subcutaneous injection of tuberculin, and the emerging ELISPOT immunodiagnosis method. The emerging ELISPOT diagnostic method can distinguish people who are infected with tuberculosis and those who have only been injected with BCG, so compared with traditional methods, the sensitivity and specificity are greatly improved. [0003] The technical principle of the ELISPOT method for diagnosing tuberculosis is as follows: take the blood of the diagnosed person, separate the lymphocytes in the blood, and then stimulate these cells with the specific antig...

Claims

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Application Information

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IPC IPC(8): C07K7/08G01N33/535G01N33/569
Inventor 朱义鑫
Owner SHENZHEN DAKEWE BIOENG
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