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Polypeptide for specificity detection of tubercle bacillus infection contamination and reagent kit thereof

A Mycobacterium tuberculosis, specific technology, applied in the field of immunology, can solve the problems of high false negative, insufficient peptide stimulation intensity, easy to miss tuberculosis patients, etc., and achieve the effect of reducing the false positive rate

Inactive Publication Date: 2011-05-25
SHENZHEN DAKEWE BIOENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But if this polypeptide is removed, the stimulation intensity of the remaining polypeptide is not enough, and it is easy to miss the detection of real tuberculosis patients, that is to say, the false negative is high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A total of 58 healthy volunteers were selected, and all of them were negative in the traditional tuberculin skin test. The lymphocytes in the blood of each healthy volunteer were extracted by conventional methods, and their lymphocytes were divided into two parts, which were tested by ELISPOT for tuberculosis.

[0025] The specific steps were carried out according to the method listed in the literature Selected Pool of Peptides from ESAT-6 and CFP-10Proteins for Detection of Mycobacterium tuberculosis Infection (PubMed15297485). The stimulating polypeptide library used in the first part completely adopts the polypeptide library listed in this document, which contains the polypeptide CFP-10 51-70 (Seq ID No.1). The second part uses the same polypeptide library, but replaces CFP-10 51-70 with the polypeptide DKW51-70 of the present invention (Seq ID No.2).

[0026] No healthy volunteers should have a positive reaction, and any positive reaction is a false positive. Resu...

Embodiment 2

[0028] Select 23 patients who were clinically diagnosed as tuberculosis patients. The lymphocytes in the patient's blood were extracted by conventional methods, and their lymphocytes were divided into two parts for tuberculosis ELISPOT examination.

[0029]The specific steps were carried out according to the method listed in the literature Selected Pool of Peptides from ESAT-6 and CFP-10Proteins for Detection of Mycobacterium tuberculosis Infection (PubMed15297485). The stimulating polypeptide library used in the first part completely adopts the polypeptide library listed in this document, which contains the polypeptide CFP-10 51-70 (Seq ID No.1). The second part uses the same polypeptide library, but replaces CFP-10 51-70 with the polypeptide DKW51-70 of the present invention (Seq ID No.2).

[0030] Results In the two reagent groups, there were 20 positive reactions, and the diagnostic sensitivity was exactly the same, both being 87%. This shows that compared with the exist...

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Abstract

The present invention discloses a polypeptide with tubercle bacillus immunogenicity, which contains a sequence shown by Seq ID No.2 in the sequence table. The present invention also discloses a kit containing the polypetide as above. When the polypeptide and the kit are adopted to detect the infectivity of tubercle bacillus, the false positive rate is greatly decreased, while the true positive rate can be kept unchanged.

Description

technical field [0001] The invention relates to the field of immunology, in particular to a polypeptide and a kit capable of specifically detecting Mycobacterium tuberculosis infection. Background technique [0002] Tuberculosis (including pulmonary tuberculosis) is an infectious disease that seriously threatens human health. The methods for diagnosing tuberculosis include traditional methods such as sputum smear examination, pathogen culture, tuberculin subcutaneous injection, and the emerging ELISPOT immunodiagnostic method. The emerging ELISPOT diagnostic method can distinguish people infected with Mycobacterium tuberculosis from those who have only been injected with BCG, so compared with traditional methods, the sensitivity and specificity are greatly improved. [0003] The technical principle of the ELISPOT method for diagnosing tuberculosis is as follows: take the blood of the diagnosed person, separate the lymphocytes in the blood, and then stimulate these cells wit...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K7/08G01N33/535G01N33/569
Inventor 朱义鑫
Owner SHENZHEN DAKEWE BIOENG
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