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Bacteroides uniformis L8 and application thereof in degrading agar or agar oligosaccharide

A technology of Bacteroides monomorpha and agar oligosaccharides, applied in the direction of bacteria, microorganism-based methods, microorganisms, etc., can solve problems such as disturbing physiological functions, and achieve the effect of promoting growth and regulating related symptoms

Active Publication Date: 2014-06-18
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Normal intestinal flora has many important physiological functions, such as regulation of host diabetes, hypertension, hyperlipidemia, immunity, detoxification, and anti-tumor effects. normal physiological function

Method used

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  • Bacteroides uniformis L8 and application thereof in degrading agar or agar oligosaccharide
  • Bacteroides uniformis L8 and application thereof in degrading agar or agar oligosaccharide
  • Bacteroides uniformis L8 and application thereof in degrading agar or agar oligosaccharide

Examples

Experimental program
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Effect test

Embodiment 1

[0025] The isolation and identification of embodiment 1 bacterial strain L8

[0026] (1) Preparation and molecular weight determination of agar oligosaccharides

[0027] Add 10 g of agar gel into 1 L of distilled water (10 mg / mL) and heat to dissolve, then add 1 mol / L HCl aqueous solution until the final concentration of HCl is 0.1 mol / L, heat and react in a water bath at 60°C for 1 hour, after the reaction is completed, add 1 mol / L HCl to the reaction solution L NaOH aqueous solution neutralized the pH value of the reaction solution to 7, and then filtered it with a nanofiltration membrane with a molecular weight cut-off of 200-500 Da to remove salt, and then concentrated the retentate at 50°C under reduced pressure and evaporated to dryness to obtain 8 g of agar oligosaccharides, which were designated as AO.

[0028] Take AO with 0.1mol / L Na 2 SO 4 Prepare 5mg / ml solution, use TSK-GEL G3000PWXL gel chromatographic column (30cm×7.8mm), use HP1260 high performance liquid chr...

Embodiment 2

[0054] Example 2 Degradation of Bacteroides monomorpha L8 to agar oligosaccharides

[0055] The AO in the VI-AO liquid medium in Example 1 was replaced with agar oligosaccharides (average molecular weight 0.5-10kDa) at a final concentration of 1g / L, 30g / L and 50g / L, respectively, to obtain VI-AO-1, VI-AO-30 and VI-AO-50 medium. The slant medium is the agar powder with a final concentration of 1.5wt% added to the VI-AO liquid medium in Example 1, and the seed liquid medium is the VI-AO liquid medium in Example 1.

[0056] Inoculate Bacteroides monomorpha L8 into the slant medium and culture at 37°C for 3 days to obtain the slant cells; pick a single clone from the slant cells to the seed liquid medium and culture at 37°C for 3 days to obtain the seed liquid. 6% volume ratio was inoculated into VI-AO-1, ​​VI-AO-30 and VI-AO-50 liquid medium respectively, cultured anaerobically at 37°C, cultured until 48h, 96h, 144h, 192h respectively, and analyzed the degradation , Degradation...

Embodiment 3

[0066] Example 3 Degradation of Bacteroides monomorpha L8 to agar (AP)

[0067] Replace the AO in the VI-AO liquid medium in Example 1 with AP (molecular weight is 100-300kDa), the final concentrations of AP in the medium are 0.5g / L and 1g / L respectively, and prepare VI-AP-0.5 and VI - AP-1 liquid medium. The slant medium is the agar powder with a final concentration of 1.5wt% added to the VI-AO liquid medium in Example 1, and the seed liquid medium is the VI-AO liquid medium in Example 1.

[0068] Inoculate Bacteroides monomorpha L8 into the slant medium, culture at 37°C for 3 days, pick a single clone from the slant and inoculate it into the seed medium, and cultivate it at 37°C for 3 days. The amount of inoculum was inoculated into VI-AP-0.5 liquid medium and VI-AP-1 liquid medium respectively, cultured anaerobically at 37°C, and samples were taken at 0h, 48h, 96h, 144h and 192h to analyze the degradation situation and the structure of degradation products and degradabili...

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Abstract

The invention discloses a new bacteria strain-bacteroides uniformis L8 and an application thereof in degrading agar or agar oligosaccharide. The bacteria strain can degrade agar with molecular weight of 100-300kDa into agar oligosaccharide and further degrade the agar oligosaccharide into D-galactose, or degrade the agar oligosaccharide with molecular weight of 0.5-10kDa into D-galactose finally, in addition, the agar oligosaccharide has the specificity to promote growth of the bacteroides uniformis, so as to play a role in regulating associated symptoms caused by obesity.

Description

(1) Technical field [0001] The invention relates to a degrading strain of agar or agar oligosaccharide, in particular to the application of Bacteroides uniformis L8 in degrading agar or agar oligosaccharide. (2) Background technology [0002] Agar gum is a polysaccharide derived from marine red algae, mainly composed of (l→3)-β-D-galactose (hereinafter referred to as G) and (1→4)-3,6-endether-α-L-galactose Lactose (hereinafter referred to as A) is alternately connected, and agar is widely used in the food industry as a food additive because of its special gel properties. Agar oligosaccharides (hereinafter referred to as AO) with relatively low molecular weight can be obtained by enzymatic or acid degradation of agar. Recent studies have found that agar oligosaccharides have a prebiotic effect of promoting the growth of bifidobacteria in the intestinal tract. [0003] The gastrointestinal tract of healthy people hosts a wide variety of intestinal flora, about 10 14 about. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P19/14C12P19/02C12R1/01
Inventor 王欣于广利李苗苗尹业师
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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