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multi-stage rotating speed regulating policy for improving pseudomonas denitrificans fermentation for production of vitamin B12 by

A Pseudomonas, multi-stage technology, applied in the direction of fermentation, microorganisms, microorganism-based methods, etc., can solve the problems of high cost, many by-products, difficult to purify, etc., to achieve increased yield, improved permeability, and improved effective utilization Effect

Inactive Publication Date: 2018-12-07
GUANGJI PHARMA MENGZHOU
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Production of vitamin B by chemical synthesis 12 The cost of the method is high, the by-products are many, and it is difficult to purify

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Multi-stage rotation speed regulation strategy to improve the fermentation of Pseudomonas denitrification to produce vitamin B 12 , including the following steps:

[0022] 1) Fermentation and cultivation of Pseudomonas denitrification strains in fermentation medium to produce vitamin B 12 , the fermentation medium used contains: sucrose 80g / L, corn steep liquor 45g / L, betaine 14g / L, ammonium sulfate 1g / L, potassium dihydrogen phosphate 0.6g / L, cobalt chloride 0.06g / L, oxidation Magnesium 0.5g / L, 5,6-dimethylbenzimidazole 0.05g / L, zinc sulfate 0.06g / L, calcium carbonate 0.8g / L, auxin 0.03g / L, tryptophan 0.04g / L and Glutamine is 0.08g / L, and the pH of the fermentation medium is 7.2-7.4; during the fermentation process, the stirring speed is controlled in stages, the stirring speed is 160rpm during the 0-25h of fermentation, and the stirring speed is 160rpm during the 26-90h of fermentation. 370rpm, 91-185h of fermentation, stirring speed of 100rpm; fermentation conditio...

Embodiment 2

[0026] Multi-stage rotation speed regulation strategy to improve the fermentation of Pseudomonas denitrification to produce vitamin B 12 , including the following steps:

[0027] 1) Fermentation and cultivation of Pseudomonas denitrification strains in fermentation medium to produce vitamin B 12 , the fermentation medium used contains: sucrose 75g / L, corn steep liquor 40g / L, betaine 12g / L, ammonium sulfate 0.8g / L, potassium dihydrogen phosphate 0.5g / L, cobalt chloride 0.05g / L, Magnesium oxide 0.4g / L, 5,6-dimethylbenzimidazole 0.04g / L, zinc sulfate 0.05g / L, calcium carbonate 0.8g / L, auxin 0.02g / L, tryptophan 0.02g / L and glutamine 0.06g / L, the pH of the fermentation medium is 7.2-7.4; during the fermentation process, the stirring speed is controlled in stages, the 0-25h of fermentation, the stirring speed is 150rpm, the 26-90h of fermentation, the stirring speed 300rpm, 91-185h of fermentation, stirring speed of 100rpm; fermentation conditions also include: fermentation temper...

Embodiment 3

[0031] Multi-stage rotation speed regulation strategy to improve the fermentation of Pseudomonas denitrification to produce vitamin B 12 , including the following steps:

[0032] 1) Fermentation and cultivation of Pseudomonas denitrification strains in fermentation medium to produce vitamin B 12 , the fermentation medium used contains: sucrose 85g / L, corn steep liquor 50g / L, betaine 18g / L, ammonium sulfate 1.2g / L, potassium dihydrogen phosphate 0.8g / L, cobalt chloride 0.08g / L, Magnesium oxide 0.6g / L, 5,6-dimethylbenzimidazole 0.06g / L, zinc sulfate 0.08g / L, calcium carbonate 1.0g / L, auxin 0.05g / L, tryptophan 0.05g / L and glutamine 0.1g / L, the pH of the fermentation medium is 7.2-7.4; during the fermentation process, the stirring speed is controlled in stages, the stirring speed is 200rpm for 0-25h of fermentation, and the stirring speed is 26-90h for fermentation. 380rpm, 91-185h of fermentation, stirring speed of 120rpm; fermentation conditions also include: fermentation temp...

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Abstract

The invention belongs to the technical field of biological fermentation and discloses a multi-stage rotating speed regulating policy for improving pseudomonas denitrificans fermentation for productionof vitamin B12. A pseudomonas denitrificans strain is fermented and cultured in a fermenting culture medium. One litter of the culture medium contains 70-85g of saccharose, 40-55g of corn steep liquor, 10-20g of glycine betaine, 0.02-0.05g of auxin, 0.01-0.05g of tryptophan and 0.05-0.1g of glutamine and the like; if the fermentation time is 0-25h, the stirring rotating speed is 150-280 rpm; if the fermentation time is 26-90 h, the stirring rotating speed is 300-400 rpm; if the fermentation time is 91-185h, the stirring rotating speed is 100-200 rpm; vitamin B12 is separated and purified fromfermentation liquor. By controlling the stirring rotating speed and the components of the fermenting culture media, the output of vitamin B12 is improved.

Description

technical field [0001] The invention belongs to the technical field of biological fermentation, and in particular relates to a multi-stage rotational speed control strategy to improve the production of vitamin B by fermentation of Pseudomonas denitrification bacteria 12 . Background technique [0002] Vitamin B 12 Also known as cobalamin, it is an important nutritional factor for animals and humans. It is widely used in feed, food and medical and health fields. It has significant effects in many aspects, such as promoting the development and maturation of red blood cells, preventing pernicious anemia; maintaining Nervous system health; exists in the form of coenzymes, can increase the utilization rate of folic acid, promote the metabolism of carbohydrates, fats and proteins; can also promote protein synthesis, which plays an important role in the growth and development of infants; it can also eliminate restlessness, Concentrate, enhance memory and sense of balance, and vit...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/42C12R1/38
CPCC12P19/42
Inventor 胡勇干王鑫刘延茂李会
Owner GUANGJI PHARMA MENGZHOU
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