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Chrysene degradation bacterium and application thereof

A technology of bacterial strains and strains, applied in the field of biodegradation treatment of contaminated soil and water, can solve the problems of lack of efficient polycyclic aromatic hydrocarbon degrading bacteria, restricting development and application, etc.

Inactive Publication Date: 2015-06-03
BEIJING NORMAL UNIVERSITY
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  • Abstract
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  • Claims
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Problems solved by technology

[0003]However, due to the lack of efficient PAH-degrading bacteria, the further development and application of PAH-contaminated soil bioremediation technology is restricted

Method used

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  • Chrysene degradation bacterium and application thereof

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Embodiment Construction

[0019] to join Oil-contaminated soil was added to the open glass bottle of basal medium Enrichment and acclimatization of degrading microorganisms, added after 30 days The conical flasks of basal culture medium, trace metal solution, vitamin c solution and adsorbent are inserted into enriched and domesticated soil. After culturing in a shaker at a temperature of 25-30°C and a rotation speed of 100r / min for 5-7 days, the cycle transfer is carried out, and it can be efficiently removed after the number of cycles is greater than 8 times. of degrading bacteria. Made in a clean bench The bottom plate of agar solid medium as the only carbon source and energy source, on the bottom plate, make a plate containing Degrade the agar solid medium top plate of the bacterial population, and culture the prepared medium plate in an incubator for 10 to 15 days. In the ultra-clean bench to join , basal medium, trace metal liquid, vitamin c solution and adsorbent conical flasks were ...

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Abstract

The invention belongs to the technical field of biological degradation treatment, and discloses a bacterial strain with a degradation function on chrysene. A chrysene degradation bacterium is identified to be ochrobactrum anthropic DW1, and the bacterium strain preservation number is CGMCC No.8621. The bacterium strain is made into bacterium suspension with the bacterium concentration of 1.25*108 CFU (colony forming unit) / ml, and the degradation and removal rate of chrysene with the original concentration of 0.14mg / L is 56.2% after seven days.

Description

technical field [0001] The invention belongs to the technical field of biodegradation treatment of polluted soil and water, in particular to a plant degrading bacteria. Background technique [0002] Polycyclic aromatic hydrocarbons (Polycyclic Aromatic Hydrocarbons, PAHs) are a class of condensed ring compounds containing two or more benzene rings arranged in linear, angular or clustered shapes, with hydrophobicity, low solubility, low vapor pressure, High melting point and boiling point, high octanol-water partition coefficient and other characteristics. Polycyclic aromatic hydrocarbons have strong carcinogenic, teratogenic and mutagenic effects. Generally speaking, low-molecular-weight PAHs with 2-3 rings have significant acute toxicity, while some high-molecular-weight PAHs have potential carcinogenicity. Due to the presence of conjugated large π-bond electron systems in PAHs molecules, they have high stability, their photolysis and biodegradation are slow, and they ca...

Claims

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Application Information

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IPC IPC(8): C12N1/20B09C1/10C12R1/01
CPCB09C1/10C12N1/205C12R2001/01
Inventor 朱宜秦伟谢恩郑蕾丁爱中豆俊峰
Owner BEIJING NORMAL UNIVERSITY
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