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A method for efficiently degrading azoxystrobin by using Pallidum pallidus

A technology of Bacillus pallidum and azoxystrobin, which is applied in the field of efficient degradation of azoxystrobin, can solve the problem of low efficiency of microbial degradation of azoxystrobin, and achieve the effect of reducing the number of experiments

Active Publication Date: 2017-09-26
SOUTH CHINA AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention is to overcome the defect of low efficiency of using microorganisms to degrade azoxystrobin in the prior art, and to provide a method for efficiently degrading azoxystrobin by using P.

Method used

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  • A method for efficiently degrading azoxystrobin by using Pallidum pallidus
  • A method for efficiently degrading azoxystrobin by using Pallidum pallidus
  • A method for efficiently degrading azoxystrobin by using Pallidum pallidus

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Experimental program
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Effect test

Embodiment 1

[0029] Embodiment 1 single factor investigation experiment

[0030] Select temperature, pH value, strain inoculum amount, liquid loading amount, and shaking rate as the main factors affecting the degradation rate of azoxystrobin, and change the above-mentioned factors in turn to investigate the effect of the degrading bacteria Ochrobactrum anthropi SH14 on azoxystrobin. The results showed that the three factors of temperature, pH value and strain inoculum had a significant impact on the degradation rate of azoxystrobin.

Embodiment 2

[0031] Example 2 Response surface optimization experiment

[0032] According to the results of the single factor experiment, the three factors of temperature, pH value and bacterial inoculum were selected for optimal design of the response surface, and the experimental design was carried out according to the principle of Central Composite Rotatable Design (CCRD) by using SAS 9.0 statistical software (Table 1 ), with temperature (X 1 ), pH value (X 2 ) and inoculum amount (X 3 ) as the independent variable, with the degradation rate of azoxystrobin as the response value (Y 1 ), to establish the multiple quadratic regression equation:

[0033] Y 1 =86.49+1.39X 1 +2.05X 2 +1.0X 3 -11.22X 1 2 -1.2X 1 x 2 -0.13X 1 x 3 -3.23X 2 2 -0.53X 2 x 3 -1.78X 3 2

[0034] Table 1 Response surface central composite rotation design (CCRD) experiment

[0035]

[0036] The statistical analysis results are shown in Tables 2-4. The statistical analysis results show that the ...

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Abstract

The invention belongs to the technical field of biology and particularly discloses a method for efficiently degrading azoxystrobin by utilizing ochrobactrum anthropic. Ochrobactrum anthropic SH14 is used for degrading the azoxystrobin under the condition that the temperature is 30.2 DEG C, the pH is 7.9 and the inoculum size is 0.2g / L, and the degradation rate of the ochrobactrum anthropic SH14 on 50mg / L of azoxystrobin is above 85%; the method disclosed by the invention provides theoretical practice basis for future large-scale industrial production and application of the ochrobactrum anthropic SH14 and has wide signification.

Description

technical field [0001] The invention relates to the field of biotechnology, and more specifically relates to a method for efficiently degrading azoxystrobin by using Pallidum bacillus. Background technique [0002] Azoxystrobin is a commonly used methoxyacrylate pesticide. This pesticide has been widely used to prevent and control crop diseases and is one of the main varieties in the pesticide market. However, in recent years, studies have found that azoxystrobin residues are potentially harmful to non-target organisms, such as high toxicity to bees, fish and aquatic invertebrates, chronic toxicity to humans and other mammals, long-term exposure or ingestion Will lead to chronic diseases, and even have the risk of carcinogenic, teratogenic, mutagenic. In view of the widespread use of azoxystrobin and its residual hazards, it is particularly necessary to eliminate the residual pollution of azoxystrobin. [0003] Microbial degradation technology has now become an effective ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A62D3/02A62D101/04A62D101/26A62D101/28
CPCA62D3/02A62D2101/04A62D2101/26A62D2101/28
Inventor 陈少华廖立胜陈昱帆王惠杉冯起顺
Owner SOUTH CHINA AGRI UNIV
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