A kind of Pallidus tritici capable of degrading lincomycin

A technology of Paleobacterium pallidum and lincomycin, applied in the field of microorganisms, to achieve the effects of eliminating adverse effects, efficient treatment methods, and saving resources

Active Publication Date: 2017-08-22
HEBEI UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, in the prior art, there is no method for harmless treatment of residual lincomycin in bacterial residues. Therefore, how to use green and environmentally friendly means to carry out appropriate harmless treatment of residual lincomycin in bacterial residues and effectively Utilization has become a technical problem in related industries

Method used

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  • A kind of Pallidus tritici capable of degrading lincomycin
  • A kind of Pallidus tritici capable of degrading lincomycin
  • A kind of Pallidus tritici capable of degrading lincomycin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1 Screening of Pallidinus tritici strains.

[0021] Step 1: Collect 10 g of sludge from the vicinity of an antibiotic enterprise in Shijiazhuang, inoculate it into 100 mL of inorganic salt medium containing 20 mg of lincomycin, and culture it on a shaker at 30° C. and 200 rpm. Transfer once every 7 days after the start of culture, and transfer to a medium containing lincomycin at a higher concentration with a 10% (V / V) inoculum during transfer, and the drug concentration of lincomycin in turn 20mg / L, 40mg / L, 80mg / L, 160mg / L, and so on, to 640mg / L, to obtain different concentrations of enriched culture solution containing bacteria.

[0022] Wherein, the composition and the proportioning ratio of the inorganic salt culture medium among the present invention are (g / L): K 2 HPO 4 1.6g, KH 2 PO 4 0.4g, MgSO 4 ·7H 2 O 0.2g, CaCl 2 2H 2 O 0.025g, FeCl 3 ·6H 2 O 0.0023, NH 4 NO 3 0.5g, 18.0g agar, adjust the pH value to 7.0 with 1mol / L HCL or NaOH, and aut...

Embodiment 2

[0029] Example 2 Screening of the Ochrobactrum tritici strain of the present invention and its degradation effect on lincomycin.

[0030] Step 1: Take 10g of sludge and put it in a sterilized 250mL Erlenmeyer flask, add 100mL of lincomycin hydrochloride solution with a concentration of 2000mg / L, and culture on a shaker at 30°C with a rotation speed of 200r / min. After the culture starts, transfer once every 7 days, inoculate into fresh lincomycin hydrochloride solution acclimation medium with 10% inoculum during transfer, and gradually increase the content of lincomycin hydrochloride. Detect the degradation rate of lincomycin hydrochloride, and select the best group for the isolation of strains.

[0031] Step 2: the bacterial suspension (1 × 108 cells / mL) were inserted into 100 mL of inorganic salt medium containing 20 mg / L lincomycin, and three parallels were taken, and the inorganic salt medium containing no bacteria but containing the same concentration of lincomycin was use...

Embodiment 3

[0036] Example 3: Degradation of lincomycin in the bacteria residue by the strain of P. tritici.

[0037] Pass lincomycin waste wet hyphae through a 0.2 mm sieve, and sterilize at 121°C for 30 minutes. Weigh 10 g of sterilized waste mycelium, put it into 100 mL of water, add 5 g (dry weight) of Pallidinus tritici thalline that has been cultivated for 48 hours to the waste wet mycelium solution, and set up 3 groups of parallel experiments to add bacteria The fermented culture broth of P. tritici without adding P. tritici was used as a control, and samples were taken after 16, 32, 48, and 60 hours of treatment, and the residual amount of lincomycin was detected by high performance liquid chromatography. The results show that the degradation rate of the strain to lincomycin can reach 100% after being cultivated for 48 hours.

[0038] Table 2 Degradation effect of Pallidalbacterium tritici strains on lincomycin in bacteria residue

[0039]

[0040] As can be seen from the abo...

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Abstract

The invention discloses wheat ochrobactrum of degradable lincomycin. The wheat ochrobactrum is preserved in China General Microbiological Culture Collection Center as the preservation number CGMCC No.10665. The wheat ochrobactrum of the degradable lincomycin can use the lincomycin as an exclusive carbon source to grow, and the degradation capacity of the strain for the lincomycin can reach 100 percent in 24 hours. The strain can be used for harmlessly treating fermented fungi residue in the industrial production of the lincomycin, the treatment method is effective, environment-friendly, capable of saving the resource, low in treatment cost and capable of thoroughly eliminating adverse impact of lincomycin waste hypha on the environment.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a Pallidus tritici capable of degrading lincomycin. Background technique [0002] Lincomycin is an alkaline broad-spectrum antibiotic produced by Streptomyces lincolnii, which is widely used to treat inflammation caused by Gram-positive bacteria. [0003] At present, the industrial production of lincomycin mainly uses starch, corn steep liquor, bean cake powder, etc. as the medium, and is fermented, extracted and purified by Streptomyces. The mycelia part of the by-product formed after the fermentation broth is pretreated for solid-liquid separation is the production waste residue, which contains lincomycin residue, crude protein, cellulose and crude fat. Due to the stable physical and chemical properties of residual lincomycin in the waste residue, it has long-term residue and bioaccumulation, and it is easy to cause various safety hazards such as biological drug resista...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20B09B3/00C12R1/01
Inventor 刘守信王鹏焦有景冯娟黄净
Owner HEBEI UNIVERSITY OF SCIENCE AND TECHNOLOGY
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