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Microbial agent and its application in zearalenone degradation

A technology of zearalenone and bacterial agent, applied in the field of microorganisms, can solve the problems of long degradation time and the need to improve the degradation rate

Active Publication Date: 2018-07-06
COFCO NUTRITION & HEALTH RES INST +5
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It can be seen that the existing methods for microbial degradation of ZEN take a long time to degrade, and the degradation rate still needs to be improved.

Method used

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  • Microbial agent and its application in zearalenone degradation
  • Microbial agent and its application in zearalenone degradation
  • Microbial agent and its application in zearalenone degradation

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preparation example Construction

[0036] The present invention also provides a preparation method of the above bacterial agent, the method comprising: separately cultivating white rot fungi and bacilli separately, and mixing the obtained white rot fungi and bacilli to obtain the bacterial agent of the present invention, wherein, the dry weight In total, the weight ratio of the white rot fungus to the bacillus is 1:2-50, preferably 1:5-40.

[0037] In the second aspect, the present invention also provides the application of the above bacterial agent in removing zearalenone.

[0038] In the third aspect, the present invention also provides a grain, oil or feed, wherein the grain, oil or feed contains the above bacterial agent.

[0039] In a preferred situation, relative to 1 kg of grain, oil or feed, the content of the bacterial agent may be at least 0.001 g, preferably at least 0.01 g, more preferably at least 0.1 g, and even more preferably at least 1 g by dry weight.

[0040] In the present invention, the te...

preparation example 1

[0062] Bacillus subtilis (preservation number is CGMCC NO.13138) was inoculated in liquid LB medium with 1% inoculation amount, cultivated at 150rpm and 30°C, and after cultivating for 48h, the viable cell concentration was 1×10 9 CFU / mL of bacterial liquid, and then the obtained bacterial liquid is made into dry powder, wherein the number of live bacteria per gram of dry powder is 1×10 9 CFU, that is, preparation 1 was obtained.

preparation example 2

[0064] Bacillus subtilis (preservation number is CGMCC NO.9463) was inoculated in liquid LB medium with 1% inoculation amount, cultivated at 150rpm and 30°C, and after cultivating for 48h, the concentration of viable cells was 1×10 9 CFU / mL of bacterial liquid, and then the obtained bacterial liquid is made into dry powder, wherein the number of live bacteria per gram of dry powder is 1×10 9 CFU, formulation 2 was obtained.

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Abstract

The invention relates to the technical field of microorganisms, and discloses a microbial agent, its application, a grain and oil or feed, and a method for removing zearalenone. The microbial agent contains a white-rot fungus and a bacillus, and a dry weight ratio of the white rot fungus to the bacillus is 1:(2-50). The white rot fungus and the bacillus are combined, so the obtained microbial agent can efficiently and safely remove the zearalenone from the grain and oil and / or the feed, and achieves a zearalenone removal rate especially when an appropriate amount of a copper agent is added tothe microbial agent. Additionally, the zearalenone can be completely removed from the grain and oil and / or the feed through combining the spore preparation of the white rot fungus with the spore preparation of the bacillus.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to a bacterial agent and its application in removing zearalenone, grain, oil or feed containing the bacterial agent and a method for removing zearalenone. Background technique [0002] Zearalenone (Zearalenone, ZEN) is a mycotoxin produced by Fusarium fungus and released into the soil environment. It is the most widely polluted Fusarium toxin in the world. In 1999, D. Mello et al. found that ZEN can reduce the embryo survival rate and the birth rate of newborn fetuses in pregnant animals. The impact of ZEN on the human body is mainly to induce tumors, induce DNA shrinkage, and cause chromosomal abnormalities. In addition, ZEN can bind to 17β-estradiol receptors, leading to fat oxidation, cell apoptosis, inhibition of protein and DNA synthesis, and inhibition of biosynthesis of other macromolecules. [0003] Zearalenone, as one of the most widespread mycotoxins that contaminate grain,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N1/20A23K10/18A23L5/20C12R1/645C12R1/125C12R1/10C12R1/225C12R1/23C12R1/24C12R1/245C12R1/15C12R1/01
CPCC12N1/14C12N1/20
Inventor 林海龙苏会波李凡陈博周勇郭翠佟易李义李久仁樊维荣刘劲松杨勇靳玉双范洪岩
Owner COFCO NUTRITION & HEALTH RES INST
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