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Bacteroides ovatus for relieving endotoxin infection and application of bacteroides ovatus

A technology of Bacteroides ovale and Bacteroides, applied in the field of microorganisms, to achieve the effects of increased concentration, wide application prospects, and low immunogenicity

Active Publication Date: 2018-10-12
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no patent document reporting the alleviating effect of Bacteroides ovale on endotoxin infection

Method used

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  • Bacteroides ovatus for relieving endotoxin infection and application of bacteroides ovatus
  • Bacteroides ovatus for relieving endotoxin infection and application of bacteroides ovatus
  • Bacteroides ovatus for relieving endotoxin infection and application of bacteroides ovatus

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] The cultivation and storage methods of Bacteroides ovatus CCFM1021 are as follows:

[0046] Preparation of culture medium: Weigh brain-heart infusion BHI medium (such as the product of Qingdao Haibo Biotechnology Co., Ltd.) in proportion, dissolve it in distilled water, and add cysteine ​​hydrochloride 1g / L, hemochloride Vegetables 0.01 g / L, vitamin K 10.002 g / L, mix evenly, then adjust the pH to 7.0, and sterilize at 115-121° C. for 15-20 minutes to obtain the culture medium.

[0047] Cultivation method: Bacteroides ovale CCFM1021 strains are inoculated according to the inoculum amount of 2-4% based on the weight of the above-mentioned culture medium, and anaerobically cultivated at 37°C for 12-18 hours, reaching a stable period.

[0048] Preparation of the protective agent: Weigh 1 g / L of cysteine ​​hydrochloride and 200 g / L of glycerol, dissolve them in distilled water uniformly, and sterilize at 115-121° C. for 15-20 minutes to obtain the protective agent.

[0049]...

Embodiment 2

[0050] Embodiment 2: Tolerance dose experiment of Bacteroides ovale CCFM1021 gavage mice

[0051] Get 10 healthy female C57 mice of 6-8 weeks of age, the cryopreservation agent (10 10 CFU / ml), observed for 5 days, and recorded the body weight and death status of the mice.

[0052] The experimental results are listed in Table 1. These results indicate that feeding concentrations of 10 9 CFU of Bacteroides ovale CCFM1021 had no significant effect on the mice, with weight gain and no death. The appearance of the mice had no obvious pathological symptoms.

[0053] Table 1: Feeding 10 9 Effect of CFU of Bacteroides ovale CCFM1021 on body weight of mice

[0054]

Embodiment 3

[0055] Example 3: Immune tolerance experiment of mice fed with Bacteroides ovale CCFM1021

[0056] Twenty healthy female C57 mice aged 6-8 weeks were randomly divided into two groups: negative control group and Bacteroides ovale CCFM1021 intervention group, with 10 mice in each group. The mice of the Bacteroides ovale CCFM1021 intervention group were given 0.1ml of the above-mentioned Bacteroides ovale CCFM1021 cryopreservation agent once every 24 hours (10 10 CFU / ml), the mice in the control group were fed with 0.1ml of the protective agent of the above-mentioned cryopreservation agent every 24 hours, and all mice were sacrificed after 5 days of continuous gastric administration, and the blood and colon tissues were respectively collected and passed through RIPA lysate (Beiyuntian Biotechnology Co., Ltd. Technology Co., Ltd.) after lysing and homogenizing, the concentration of immune factors was determined using an ELISA kit (product of Nanjing Senbega Biotechnology Co., Ltd....

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Abstract

The invention discloses bacteroides ovatus for relieving endotoxin infection and an application of the bacteroides ovatus, and belongs to the field of microbial technology. The bacteroides ovatus CCFM1021 provided by the invention has low immunogenicity, and the bacteroides ovatus CCFM1021 is capable of reducing content of proinflammatory factors in blood of mice of endotoxin infection, up-regulating the number of Foxp3+ regulatory T cells and stabilizing intestinal flora composition. The bacteroides ovatus CCFM1021 can be used for preparing a pharmaceutical composition for relieving the endotoxin infection; and the bacteroides ovatus has a broad application prospect.

Description

technical field [0001] The invention relates to a Bacteroides ovale for alleviating endotoxin infection and application thereof, belonging to the technical field of microbes. Background technique [0002] Endotoxin, also known as lipopolysaccharide, is an important component of the cell wall of Gram-negative bacteria. Endotoxin has a stable structure and is mainly composed of three parts: polysaccharide O antigen, core polysaccharide and lipid A (lipid A). Lipid A is a lipidated glucosamine disaccharide that is pyrogenic and a toxic component of Gram-negative bacterial endotoxins. Under normal circumstances, endotoxins are only released when the bacteria die and dissolve or the bacterial cells are destroyed by artificial methods, and the structure of endotoxins is stable. biological activity. [0003] The intestinal lumen of a normal body contains a large number of intestinal bacteria, and the intestinal mucosa can selectively absorb nutrients and prevent the entry of mic...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/04A61K35/741A61P31/04A61P39/02C12R1/01
CPCA61P31/04A61P39/02C12N1/04C12N1/20A61K2035/115C12R2001/01C12N1/205A61K35/741
Inventor 陈卫翟齐啸谭惠子陆文伟田丰伟赵建新张灏
Owner JIANGNAN UNIV
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