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Microbial flora for decomposing dimethylformamide and culture method thereof

A technology of dimethylformamide and cultivation method, which is applied in the field of decomposing dimethylformamide, can solve problems such as the difficulty of degrading dimethylformamide wastewater, and achieve the effect of reducing operating costs

Inactive Publication Date: 2018-08-17
EAST CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the chemical properties of dimethylformamide are stable, the chemical formula is (CH3)2NHCHO, and its molecular ends are two strong hydrophobic methyl groups, and the BOD / COD is 0.065, which can inhibit the biological treatment of wastewater. Therefore, It is very difficult to degrade dimethylformamide wastewater by ordinary activated sludge method

Method used

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  • Microbial flora for decomposing dimethylformamide and culture method thereof
  • Microbial flora for decomposing dimethylformamide and culture method thereof
  • Microbial flora for decomposing dimethylformamide and culture method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] (1) In the ultra-clean bench, configure 100mL medium (10% inoculum rate) in the Erlenmeyer flask: 10mL+2mL10% dimethylformamide+1mL CaCl2+20μL FeSO4+87mL nutrient solution, adjust the pH to 7.0 , after the configuration is completed, use Para sealing film for sealing;

[0043] (2) The Erlenmeyer flask was cultured in a shaker at 30°C, and the rotation speed of the shaker was 150r / min. As the reaction progressed, the pH of the culture system gradually increased, so the pH was adjusted to 7.0 every 24 hours. Measure the OD600 of the reaction system every 48h (measure the concentration of bacteria or fungi according to OD600), and Concentration, based on system OD changes and nitrogen concentration changes to determine the reaction process, adjust the length of the reaction cycle. pH adjustment and sampling are completed in the ultra-clean bench;

[0044] (3) Determine the culture end point of each cycle: A large number of studies have shown that the decomposition of...

Embodiment 2

[0047] Embodiment 2 mixed flora batch test:

[0048] After completing 11 cycles of cultivation and acclimatization, a total of 4 groups of batch experiments were set up for the characteristics of the mixed flora. Figure 1-4 The vertical axis of is the concentration of ammonia nitrogen obtained by the decomposition of dimethylformamide.

[0049] (1) Effect of inoculation rate on the activity of flora

[0050] like figure 1 As shown, five inoculation rates were set in the test: 10%, 20%, 30%, 40% and 50%. The reaction volume was maintained at 100mL during configuration, and the volume of the bacterial solution was increased step by step from 10mL to 50mL. At the same time, the nutrient solution was Decrease from 87mL to 47mL step by step, the cycle time is controlled to 48h, and the remaining components and control conditions are consistent with the cultivation and acclimation stage. like figure 1 As shown, the optimal inoculation rate of the mixed flora is 30%, and the dim...

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Abstract

The present invention relates to a method for decomposing dimethylformamide, and more particularly to a microbial flora for decomposing the dimethylformamide and a culture method thereof. The microbial flora for decomposing the dimethylformamide includes: Brevundimonas vesicularis, Brevibacillus parabrevis, achromobacter, Flavobacterium, Stenotrophomonas, Ochrobactrum anthropi, Aquamicrobium defluvii, Sphingobacteria and Nocardioides. The microbial flora utilizes the dimethylformamide as a carbon source and a nitrogen source to oxidatively decompose the dimethylformamide while satisfying the own growth of the microbial flora; the microbial flora can efficiently remove the refractory organic matter dimethylformamide in industrial wastewater, and reduces the operating costs of wastewater treatment.

Description

technical field [0001] The invention relates to a method for decomposing dimethylformamide, in particular to a bacterial group for decomposing dimethylformamide and a culture method thereof. Background technique [0002] Dimethylformamide is an organic solvent with excellent performance and a common main fine chemical raw material. It has a wide range of industrial uses and has important applications in the polyurethane synthetic leather industry, pharmaceuticals, pesticides, petrochemicals, papermaking, and textiles. Since dimethylformamide is only used as an organic solvent without chemical reaction, there is almost no loss in quantity, so it is easy to form high-concentration dimethylformamide organic industrial wastewater. [0003] At present, domestic scholars mainly use physical and chemical methods to treat dimethylformamide wastewater, such as activated carbon adsorption, extraction, and Fenton oxidation. Wastewater needs further treatment. Since microorganisms pla...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/01C12R1/025C12R1/20C02F101/38
CPCC02F3/34C02F2101/38C12N1/20
Inventor 张艳梅李泽兵张鹤安凯阮书瑜吴双谢荣福唐慧彬吴清宇肖俊伟
Owner EAST CHINA UNIV OF TECH
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