High-tolerance phenylamine degrading bacterium and application thereof

An aniline-degrading bacteria and tolerance technology, applied in the direction of bacteria, water/sludge/sewage treatment, biochemical equipment and methods, etc., can solve the problems of ecological environment pollution, refractory degradation, high toxicity, etc., to reduce treatment costs, The effect of high application value and broad application prospects

Active Publication Date: 2015-04-29
NANJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Aniline toxic pollutants are highly toxic and difficult to de

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1: This example illustrates the method for obtaining highly tolerant aniline-degrading bacteria MC-01 strain.

[0030] In this example, the aniline-degrading bacteria MC-01 was obtained from the activated sludge in the aeration tank of the sewage treatment workshop of Nanjing Oil Refinery through primary screening and then secondary screening. The primary screening is based on the growth of the strains in the high-concentration aniline medium to screen out bacteria with a short adaptation period and strong metabolism. Based on the higher aniline degradation rate, high tolerance strains with better degradation efficiency were screened out.

[0031] where each liter of aniline selection medium consists of KH 2 PO 4 2 g, MgSO 4 1 g, NaCl 2 g, purified agar, 18 g, distilled water 1000 mL, heated to dissolve, sterilized at 121 °C for 20 min, and when the culture medium cooled down to about 50 °C, add 1000 mg of sterile aniline in a clean bench and mix well.

...

Embodiment 2

[0043] Embodiment 2 This embodiment illustrates the physiological and biochemical characteristics of the aniline-degrading bacteria MC-01 strain of the present invention

[0044] The strain of the invention is a Gram-negative aerobic bacterium, rod-shaped; milky-white colonies are formed on the beef extract peptone medium, round, translucent, smooth in surface, round and smooth in edge, and easy to provoke.

[0045] In this embodiment, the high-tolerant aniline-degrading bacteria MC-01 is routinely identified physiologically and biochemically on the highly-tolerant aniline-degrading bacteria MC-01 according to the "Bergey's Bacteriological Identification Manual". Indole test positive, ammonia production test positive, methyl red test reaction negative, starch hydrolysis negative, acetyl methyl alcohol test negative, nitrate reduction reaction negative, citric acid utilization positive, glucose utilization, ethanol or aldose oxidation.

[0046] In this embodi...

Embodiment 3

[0047] Embodiment 3 This embodiment illustrates the molecular biology system identification of aniline-degrading bacteria MC-01 bacterial classification of the present invention

[0048] According to "Bergey's Bacteriological Identification Manual", the routine physiological and biochemical identification of aniline-degrading bacteria MC-01 was carried out, and the total bacterial DNA was extracted by CTAB method. The obtained bacterial genomic DNA was amplified by PCR with bacterial 16SrDNA primers. After electrophoresis, the PCR product was recovered by tapping the gel. Suzhou Jinweizhi Biotechnology Co., Ltd. was entrusted to perform 16SrDNA sequencing. The results were synchronized with the 16SrDNA sequences registered in Genbank using BLAST software. According to the source comparison, it is found that the most similar species of the highly resistant aniline-degrading bacteria MC-01 obtained in this embodiment is Paleobacterium genus ( Ochrobactrum ), the homology c...

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Abstract

The invention discloses a high-tolerance phenylamine degrading bacterium MC-01(Ochrobactrum sp. MC-01), and belongs to an Ochrobactrum sp. type bacteria. The high-tolerance phenylamine degrading bacterium MC-01 is collected in the common microorganism center of the China committee for culture collection of microorganisms, the collection number is CGMCC 1.12930, and the collection date is June 10, 2014. The high-tolerance phenylamine degrading bacterium MC-01 disclosed by the invention has biodegradation function on phenylamine wastewater under the condition of high concentration; the phenylamine degradation rate of the strain can reach 99%, and no secondary environmental pollution is caused, no other chemical agents need to be added, and thus the treatment cost of the wastewater is greatly reduced and a wide application prospect is achieved. The high-tolerance phenylamine degrading bacterium MC-01 disclosed by the invention is helpful for solving the problem of a biological phenylamine wastewater treatment system under the condition of high concentration and has very high application value.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a high-tolerant aniline-degrading strain and its application. Background technique [0002] Aniline is one of the most important intermediates in the dye industry, which can be used to manufacture acid ink blue G, indigo and other dyes; it is used in the pesticide industry to produce many insecticides and fungicides; aniline is also an important raw material for rubber additives , It is also an intermediate in the production of spices, plastics, etc. Aniline toxic pollutants are highly toxic and difficult to degrade, causing serious pollution to the ecological environment. Therefore, studying the biodegradation of aniline and its derivatives is of great significance for protecting the environment and improving human health. [0003] High-concentration aniline wastewater is one of the most difficult types of industrial wastewater. There are few domestic studies on hi...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/01C02F101/38
CPCC02F3/34C02F2101/38C12N1/205C12R2001/01
Inventor 刘洋陈荧倪芳姚忠
Owner NANJING UNIV OF TECH
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