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Compound enzyme preparation and ochrobactrum assisted kitchen waste new high-temperature composting technology

A pale bacillus, high-temperature composting technology, applied in the field of microbial technology and environmental engineering, can solve the problems of difficulty in reaching higher and stable composting temperature, inability to greatly improve fermentation efficiency, and limitations, and achieve reduced scale, broad substrate spectrum, The effect of improving processing efficiency

Pending Publication Date: 2021-03-19
榆林鹤翼航天航空科技创新有限责任公司 +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] It can be seen from the above that the efficiency of current high-temperature composting technology is mainly limited to the hydrolysis of materials, resulting in that the fermentation efficiency cannot be greatly improved, and the composting temperature is difficult to achieve higher and stable

Method used

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  • Compound enzyme preparation and ochrobactrum assisted kitchen waste new high-temperature composting technology
  • Compound enzyme preparation and ochrobactrum assisted kitchen waste new high-temperature composting technology

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Example 1 Screening and Identification of Paleobacterium pallidum CGMCC No.19230

[0043] (1) Sample collection

[0044] Microbial samples were collected from high-temperature composting test samples of kitchen waste in the canteen of Yuhang Senior Middle School in Yuhang District, Zhejiang Province.

[0045] (2) Isolation, purification and preservation of strain CGMCC No.19230

[0046] Self-designed and prepared "yeast sucrose YZ medium": yeast extract 5g / L, sucrose 10g / L, peptone 10g / L, sodium chloride 5g / L, pH=7.0. Add agar 20g / L when making solid medium.

[0047] Put 1 g of the sample into an Erlenmeyer flask filled with 100 mL of sterile distilled water and some sterile glass beads, and shake it at 120 rpm (rotation per minute) for 10 minutes to obtain a suspension. Take 1mL of the suspension, dilute and spread it on the sterile YZ solid medium, place it in a constant temperature incubator at 60°C for 2-3 days, pick a single colony after obvious bacteria appear ...

Embodiment 2

[0057] Embodiment 2 compound enzyme is to the rapid heating effect of pale bacillus

[0058] Laccase (EC 1.10.3.2) powder: commercially available food-grade laccase from Jinan Dongxuan Bioengineering Co., Ltd.

[0059] Lipase (EC 3.1.1.3) powder: commercially available Longda food-grade lipase (Aspergillus oryzae, 100,000 units of enzyme activity).

[0060] Phospholipase A (EC 3.1.1.4) powder: commercially available Sigma-Aldrich phospholipase A2 P6534 enzyme 9001-84-7.

[0061] Phospholipase B (EC 3.1.1.5) powder: SUNSON / Xiasheng brand lysophospholipase (food grade).

[0062] Take 1 ton of kitchen waste, crush it to about 5cm pieces, add 20% dry and crushed straw, stir well, add 2% CGMCC No.19230 bacterial liquid, add 2‰ laccase powder and stir well Adjust pH=9.0, then add 1‰ lipase (EC 3.1.1.3) powder, 0.5‰ phospholipase A (EC 3.1.1.4) powder, 0.5‰ phospholipase B (EC 3.1.1.5) powder, keep for 5 hours After that, stir and ventilate every 30 minutes. A group without lacca...

Embodiment 3

[0063] Embodiment 3: the application of compound enzyme preparation and Pallidum in high temperature composting

[0064] 1: Kitchen waste composting

[0065] Take 2 tons of kitchen waste, and the solid-liquid mixture is cut and stirred to make the solid into pieces with a diameter of about 5cm. Put the solid and liquid garbage into the fermentation device with stirring function, and add 600Kg of dry rice husk, and mix well and evenly. Put 20Kg of CGMCC No.19230, add 3Kg of laccase powder, and stir once per minute for 60 minutes. Add 40Kg of quicklime to the compost heap several times, stir evenly so that the pH reaches 9.5, add 5Kg of esterase mixture, and stir evenly. Then the compost heap is stirred and ventilated every 30 minutes, and after maintaining for 6 hours, the temperature rises to 70°C. Put in heat-resistant bacillus for high-temperature composting, keep the temperature between 70-100°C, complete composting and fermentation after 2 days, and the weight of the ma...

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Abstract

The invention relates to the field of microbial technology and environmental engineering, and discloses a compound enzyme preparation and ochrobactrum assisted kitchen waste new high-temperature composting technology. The preservation number of the ochrobactrum is CGMCC No.19230 and the ochrobactrum is classified and named as ochrobactrum sp. The compound enzyme preparation and bacterial strain cooperated high-temperature composting technology is used for performing high-temperature aerobic composting treatment on various organic matters such as kitchen waste, agricultural product tailings, slaughter house waste and the like, so that the organic matters are completely humified, dehydrated and dried, the weight is reduced by 70-92%, and finally, an organic fertilizer with excellent qualityis formed. The new high-temperature composting technology also relates to special pretreatment means such as physical cutting, enzymatic hydrolysis, bacterial strain metabolism rapid heat production and the like, so that a compost heap can be rapidly heated to 70 DEG C or above within 8 hours, a high-temperature stage of composting is rapidly started, various high-temperature strains are rapidly turned into absolute dominant strains in a compost heap system, and on the basis, a semi-continuous compost fermentation technology capable of supplementing and discharging materials every day is realized.

Description

technical field [0001] The invention relates to the fields of microbial technology and environmental engineering, in particular to a new high-temperature composting process of kitchen waste assisted by a compound enzyme preparation and Pallidum bacterium. Background technique [0002] With the vigorous development of my country's economy and the continuous improvement of people's living standards, the output of domestic kitchen waste has increased year by year. At present, the annual output of domestic kitchen waste has exceeded 100 million tons. Kitchen waste contains a lot of organic matter, including oil, cellulose, sugar, protein and so on. If disposed of unscientifically and rationally, kitchen waste will not only produce solid pollution, but also produce serious waste water pollution and odor pollution after decay, which seriously damages the living environment and affects the lives of residents. In recent years, the high-temperature composting technology developed by...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N9/02C12N9/18C12N9/20C12N9/50C02F3/34B09B1/00C05F17/20C05F17/50C12R1/01
CPCC12N9/0061C12N9/20C12N9/18C12N9/50C02F3/34B09B1/00C05F17/20C05F17/50C05D3/02C12Y110/03002C12Y301/01003C12Y301/01004C12Y301/01005C12Y304/21064C05F5/002C05F9/00C05F1/00C05F11/00Y02W30/40
Inventor 方光玉徐有新张文武周华文范一
Owner 榆林鹤翼航天航空科技创新有限责任公司
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