Composite microbial inoculum, immobilization method and application thereof

A technology of compound microbial inoculants and immobilized cells, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., to achieve the effect of efficient degradation and strong tolerance

Inactive Publication Date: 2012-01-18
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

After retrieving patents and other relevant literature, it has not been found that the preparation of complex microbial agents by Bacillus human pallus B3 and Rhizobacillus T3 and the use of sodium alginate-PVA composite carriers to immo

Method used

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  • Composite microbial inoculum, immobilization method and application thereof
  • Composite microbial inoculum, immobilization method and application thereof
  • Composite microbial inoculum, immobilization method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1 Preparation of complex microbial agent and immobilized cell granules of human Pallidum bacillus B3 and Rhizobacillus T3

[0042] The final concentration of the inorganic culture solution of the present invention consists of: NH 4 Cl 0.04g / L, MgCl 2 ·6H 2 O 0.02g / L, K 2 HPO 4 0.1g / L, KH 2 PO 4 0.1g / L, CaCl 2 0.02g / L, glucose 0.2g / L, yeast powder 0.2g / L, pH 7.0~7.2, solvent is water, H 2 S gas is a sulfur source.

[0043] (1) Slant culture: Inoculate the slant culture medium with Ochrobactrum sp.B3 and Rhizobium sp.T3 respectively, and culture them anaerobically at 30°C for 24 hours to obtain the slant cells and Rhizobacillus T3 slant thallus; the final concentration of the slant medium consists of: yeast powder 5g / L, tryptone 10g / L, sodium chloride 10g / L, agar 15g / L, solvent is water, adjust pH to 7.0 ~7.2, sterilized at 121°C for 15 minutes;

[0044] (2) Seed cultivation: respectively inoculate the slant thalline of human paleobacterium B3 and the...

Embodiment 2

[0048] Example 2 Determination of properties of immobilized cell granules of human Pallidum B3 and Rhizobacillus T3 composite microbial agent

[0049] (1) Particle strength

[0050] Select 50 uniform particle diameters prepared by the method of Example 1 to immobilize the cell granules with the mixed microbial agent of Pseudomonas pallidus B3 and Rhizobacillus T3, add 100 mL of water, shake and cultivate at 300 r / min for 24 hours, observe the damage of the granules, and use this To describe the strength of the particles, the experimental results showed no breakage, indicating that the immobilized pellets have good mechanical strength.

[0051] (2) Particle mass transfer performance

[0052] In a 250mL small beaker, add 100mL of diluted blue ink (Ninghai Xingguang Cultural and Educational Supplies Co., Ltd.) (5mL of blue ink is added to 100mL of water), and weigh 4.0g of the mixed microbial bacteria of human Pallidus B3 and Rhizobacillus T3 prepared by the method in Example 1 ...

Embodiment 3

[0053] Example 3 Composite microbial inoculum of human paleobacterium B3 and Rhizobacillus T3 to different initial concentration H 2 Degradation performance test of S

[0054] 1) The complex microbial agent of Bacillus pallus B3 and Rhizobium T3 and the immobilized cell particles of the compound microbial agent were respectively activated in an inorganic medium at 30° C. for 24 hours, and set aside.

[0055] 2) Inoculate 5 mL of free cells and 4.0 g of compound microbial bacterial agent immobilized cell particles with the total concentration of 0.35 g / L Bacillus pallidum B3 and Rhizobium T3 compound microbial agent after activation in step (1) into 20 mL of inorganic culture In a 250mL anaerobic bottle, pH = 7.0, 30°C, 160rpm shaker for 24h, gas chromatography was used to detect H 2 The residual amount of S, see the results image 3 , H 2 The initial concentrations of S were set to 100ppm, 200ppm, 300ppm, 400ppm, and 500ppm, respectively.

[0056] Detection of H by gas chr...

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Abstract

The invention discloses a composite microbial inoculum, and an immobilization method and application thereof. The composite microbial inoculum is prepared from an Ochrobactrum sp.B3 mycetocyte microbial suspension and a Rhizobium sp.T3 mycetocyte microbial suspension, wherein the concentration of the Ochrobactrum sp.B3 mycetocyte microbial suspension is 0.15-0.2g/L, and the concentration of the Rhizobium sp.T3 is 0.15-0.2g/L. The immobilization method of the composite microbial inoculum has the advantages of simple technique, and cheap and accessible raw materials, and is beneficial to large-scale production. The composite microbial inoculum immobilized cell granules have the advantages of high mechanical strength, high elasticity and stable enzymic activity, have certain toxin immunity in a reaction system, have strong tolerance to temperature and pH value, and can still efficiently degrade H2S waste gas after being used repeatedly.

Description

(1) Technical field [0001] The present invention relates to a composite microbial bacterial agent and its immobilization method and application, in particular to a composite microbial bacterial agent of human paleobacterium B3 and rhizobium T3 and its immobilization method and application. (2) Background technology [0002] with H 2 The sulfur-containing odorous substances represented by S are extremely wide in source, high in content, highly toxic and difficult to treat. The treatment of sulfur-containing industrial waste gas is regarded as an important task of industrial waste treatment and air pollutant control at home and abroad. Biological desulfurization technology is an effective means of air pollution control. However, microorganisms usually grow in suspension in water. Due to the small density difference between them and water, they are easy to be lost from the reactor. Therefore, how to recover microorganisms from the effluent water for reuse becomes a challenge. ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N11/10C12N11/08B01D53/84B01D53/52C12R1/41C12R1/01
CPCY02A50/20
Inventor 姜理英陈建孟王惠祥吴晓薇
Owner ZHEJIANG UNIV OF TECH
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