Kit of cosmetic skincare compositions
A cosmetic composition, composition technology, applied in the field of cosmetic treatment, capable of solving problems such as maturation and poor differentiation, damage, skin barrier disruption, etc., to achieve the effect of consistent health and appearance
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Embodiment 1
[0297] Embodiment 1——Interleukin-6 (IL-6) test
[0298] The biological activity of interleukin-6 (IL-6) in skin cells was measured. IL-6 is a cytokine and is used as a biomarker of inflammation. Skin cells (keratinocytes or fibroblasts) were subjected to UV stress to induce IL-6 responses in the presence of anti-inflammatory or skin soothing agents to determine their activity in suppressing IL-6 responses.
[0299] The cultured cells (keratinocytes or fibroblasts) were taken, seeded at a density of 5000 cells per well in a cell growth medium with supplements in a 96-well plate, and incubated at 37° C. for 24 hours. Determination of cell numbers for plating purposes is performed according to standard methods known in the art.
[0300] After incubation, the cell growth medium was replaced with 100 μl of PBS without calcium and magnesium and containing the required relevant concentrations of the reagent(s). Controls included untreated irradiated and non-irradiated samples re...
Embodiment 2
[0307] Example 2 - User Observation
[0308] The user follows the following regimen twice daily for twenty-eight days: Apply a cleansing composition comprising water, betaine (0.5%), bisabolol (0.05%), and green tea extract (0.001%), followed by rinsing A cleansing composition followed by a skin balancing composition comprising water, Ophiopogon japonicus root extract (3%), green tea extract (0.0025%) and sphingosine (0.1%) followed by application of a composition comprising water, octocrylene ( 7.5%), green tea extract (0.0025%) and glycerin (3.5%).
[0309] Users find that skin is "visibly softer after only a few days," "skin looks great after two days," and skin looks "more even and brighter." It has been reported that while skin balancing compositions alone can lead to initial skin tightness, this can be alleviated by using cleansing compositions and day protection compositions in combination with skin balancing compositions. One user reported that the above regimen re...
Embodiment 3
[0310] Example 3 - User Test Study
[0311] A panel of 300 women aged 20 to 40 with mixed skin types (normal, oily, dry or combination) and mixed races were recruited. The recruited women were regular users of skin health-promoting skin preparations (ie, they used a commercially available day cream three to four times per week and a commercially available night cream three to four times per week).
[0312] The group was asked to apply a skin-balancing composition consisting of water, Ophiopogon japonicus root extract (3%), green tea extract (0.0025%), and sphingosine (0.1%) twice a day in combination with the women's usual skin care products , for four weeks. The same group was then asked to continue using the skin balancing composition described above, but also with a cleansing composition comprising water, betaine (0.5%), bisabolol (0.05%) and green tea extract (0.001%), including water , octocrylene (7.5%), green tea extract (0.0025%) and glycerin (3.5%) for daytime pro...
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