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A strain of baker's yeast and its application in the production of coenzyme I by fermentation

A baker's yeast and coenzyme technology, applied in fermentation, microorganism-based methods, microorganisms, etc., can solve the problems of low yield and purity, and no large-scale production, and achieve the effect of improving the low fermentation yield and improving the components.

Inactive Publication Date: 2017-07-18
虞龙
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, none of these methods have been put into large-scale production
Domestic Chinese Academy of Sciences Shanghai Institute of Biochemistry once used ion exchange resin to extract NAD from yeast to get about 70 grams per ton of fresh yeast (wet weight), with a purity of 70%, and the yield and purity are relatively low

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1 This example illustrates the method of mutagenizing the starting strain of baker's yeast to screen the target strain.

[0038] The baker's yeast SC-00 preserved in the laboratory was used as the starting strain for mutagenesis treatment, and the specific steps were as follows:

[0039]a) Preparation of single spore suspension: Take a cultured baker's yeast slant strain, add 10ml of sterile water, gently scrape off the spores on the surface of the agar, and place the spore suspension in a sterilized 50ml triangle In the bottle, put several sterile glass balls in advance, shake it well, filter it with sterilized absorbent cotton, and wash the filter residue with sterile water 2~3 times, finally make the volume of the filtrate reach 10ml, and count the filtrate with a hemocytometer , adjust the spore concentration to 1×10 6 a / ml;

[0040] b) Nitrogen ion implantation mutagenesis: take 100 μL of the spore suspension in step a), evenly spread it on a sterile empt...

Embodiment 2

[0048] This example illustrates the biological morphology and genetic stability of the mutagenic strain baker's yeast SC-Z180

[0049] Morphological and physiochemical properties of baker's yeast SC-Z180 of the present invention:

[0050] Colony color: milky white

[0051] Aerobic mode: facultative anaerobic;

[0052] Colony size: 1-4μm;

[0053] Growth temperature: 28-32°C;

[0054] Optimum pH: 5.4-6.5;

[0055] Colony shape: oval;

[0056] Mode of reproduction: budding;

[0057] Gram stain: Gram positive.

[0058] The results of the subculture fermentation test are shown in Table 1.

[0059] Table 1 Genetic stability of baker's yeast SC-Z180

[0060] Number of passages Coenzyme I production (g / L) 1 4.36 2 4.45 3 4.20 4 4.31 5 4.49 6 3.89

[0061] From the analysis of the results of the genetic stability experiment, it can be seen that after 6 subculture experiments, the coenzyme I produced by the fermentation of the muta...

Embodiment 3

[0063] This example illustrates the production process of coenzyme I high-yielding bacteria strain baker's yeast SC-Z180 fermented to produce coenzyme I

[0064] The culture medium formula described in the present embodiment is as follows:

[0065] Plate medium (g / L): glucose 20, fish powder peptone 20, agar 20, yeast extract 20, the rest is water, pH is natural.

[0066] Incline medium (g / L): glucose 20, fish powder peptone 20, agar 20, yeast extract 20, the rest is water, pH is natural.

[0067] Seed medium (g / L): Glucose 45, yeast extract 8, magnesium sulfate 0.1, ammonium sulfate 2, diammonium hydrogen phosphate 1, adjust the pH to 5.5 with 1mol / L hydrochloric acid, and dissolve the glucose.

[0068] Fermentation medium (g / L): glucose 50, yeast extract 10, magnesium sulfate 0.1, ammonium sulfate 2, diammonium hydrogen phosphate 1, tryptophan 3, adjust the pH to 5.5 with 1mol / L hydrochloric acid, and the rest is water, of which Glucose breakdown.

[0069] Saccharomyces c...

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PUM

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Abstract

The invention discloses a strain of baker's yeast and its application in fermentative production of coenzyme I. The baker's yeast is obtained from the original starting strain sc-00 through low-energy nitrogen ion beam implantation mutagenesis, and then through primary screening and re-screening of shake flask fermentation. Its taxonomic name is Baker's Yeast (Saccharomyces cerevisiae) SC‑Z180, which has been deposited in China Center for Type Culture Collection CCTCC, deposit number: CCTCC M 2014317. This bacterial strain can greatly increase the component of coenzyme I in the fermentation process, and reduce other components, and the obtained bacterial strain is subjected to fermentation rescreening, and this bacterial strain can greatly improve the problem of low fermentation yield. In a 5L fermenter, the coenzyme I output ratio The original starting bacteria increased by 45.8%.

Description

technical field [0001] The invention relates to a strain of baker's yeast and its application in fermentation production of coenzyme I, which belongs to the field of biotechnology. Background technique [0002] Coenzyme I (nicotinamide adenine dinucleotide, NAD+) is an important biochemical reagent, often extracted from baker's yeast. It is an important coenzyme for many reductases in organisms, used for dehydrogenation reactions of lactic acid, isocitric acid, α-ketoglutaric acid, malic acid, β-hydroxyacyl-CoA, etc. in the metabolic process of living organisms, and in the process of biological oxidation It plays the role of delivering hydrogen, activates various enzyme systems, promotes the synthesis and metabolism of nucleic acids, proteins, and polysaccharides, increases material transport and regulation control, and improves metabolic functions. It is clinically used as an adjuvant therapy for coronary heart disease, which can improve symptoms such as chest tightness an...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/18C12P19/36C12R1/865
CPCC12P19/36C12N1/185C12R2001/865
Inventor 虞龙吴奎李玉燕
Owner 虞龙
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