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Blakeslea trispora and method for preparing lycopene from blakeslea trispora

A technology of B. trispora and lycopene, which is applied in the direction of microorganism-based methods, biochemical equipment and methods, and microorganisms, and can solve the problems of low yield, low lycopene content, and high price of natural extraction methods , to achieve the effect of strong product unity, simple operation and high output

Inactive Publication Date: 2016-06-22
西藏天虹科技股份有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The content of lycopene in tomato is very low, usually only 20g of lycopene per ton of tomato, and the natural extraction method has low yield and high price, which cannot meet the market demand
Although the cost of chemical synthesis is low, it is easy to cause pollution and has certain unsafety

Method used

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  • Blakeslea trispora and method for preparing lycopene from blakeslea trispora
  • Blakeslea trispora and method for preparing lycopene from blakeslea trispora

Examples

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Embodiment 1

[0026] A kind of Blakesleatrispora H019-D, which is classified as Blakesleatrispora H019-D, which is preserved in the General Microorganism Center of China Committee for the Collection of Microorganisms. The preservation number is: CGMCCNo.11740, and the preservation time is As of: November 25, 2015; the address of the preservation unit is: No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, Institute of Microbiology, Chinese Academy of Sciences.

Embodiment 2

[0028] Such as figure 1 As shown, a method utilizing Saccharomyces cerevisiae described in Example 1 to prepare lycopene may further comprise the steps:

[0029] Strain screening process: Add 5 mL of normal saline to the culture medium with B. trispora, extract the spores with a spreader, and dissolve them in normal saline; take 1.5 mL of the spore suspension of B. trispora and centrifuge at 3000 rpm. After discarding the supernatant, add physiological acceptance, so that the concentration of the bacterial suspension reaches 107-8 / ml; take 100 μL of the bacterial suspension and add PDA-SDC-lovastatin (PDA is the abbreviation of potato dextrose agar medium, in PDA Add 1mg / 100mL of sodium deoxycholate (SDC) and 0.1mg / 100mL of lovastatin to the configuration of the medium to prepare it.) Spread on the medium, irradiate with ultraviolet light for 3min; incubate at 22°C for 48h , select a single colony with better growth, and transfer it to the PDA medium; repeat the screening thr...

Embodiment 3

[0042] Such as figure 1 As shown, a method utilizing Saccharomyces cerevisiae described in Example 1 to prepare lycopene may further comprise the steps:

[0043] Strain screening process: Add 5 mL of normal saline to the culture medium with B. trispora, extract the spores with a spreader, and dissolve them in normal saline; take 1.5 mL of the spore suspension of B. trispora and centrifuge at 5000 rpm. After discarding the supernatant, add physiological acceptance, so that the concentration of the bacterial suspension reaches 107-8 / ml; take 100 μL of the bacterial suspension and add PDA-SDC-lovastatin (PDA is the abbreviation of potato dextrose agar medium, in PDA Add 1-2mg / 100mL of sodium deoxycholate (SDC) and 0.2mg / 100mL of lovastatin to the configuration of the medium to prepare it.) Spread on the medium and irradiate it under a UV lamp for 7 minutes; at 28°C Cultivate for 60 hours, select a single colony with better growth, and transfer it to PDA medium; repeat the screen...

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Abstract

The invention discloses blakeslea trispora, which is preserved in China General Microbiological Culture Collection Center. A method for preparing lycopene from blakeslea trispora comprises the following steps of performing activation: inoculating activation strains onto a seed culture medium to be cultured for 42 to 44 under the conditions of the temperature being 30 to 35 DEG C and the rotating speed being 150 to 220rpm for obtaining a seed culture solution; inoculating the seed culture solution onto a fermentation culture medium to be fermented for 120 to 144h at 32 to 35 DEG C under the light shielding condition; then, performing fermentation for 46 to 48h; adding a blocking agent; extracting the lycopene in the fermentation solution, wherein an extraction agent is acetone or petroleum ether, the mass ratio of the fermentation solution to the extraction agent is 1: (200 to 250), the extraction temperature is 40 to 60 DEG C, and the extraction time is 2h; extracting the extraction solution at the extraction temperature being 40 to 45 DEG C; performing overnight crystallization at -4 DEG C. Through being measured by liquid phase chromatography, the purity of the lycopene is 85 percent or higher than 85 percent. The method has the advantages that the yield of the lycopene is improved; the purity of the separated lycopene is high.

Description

technical field [0001] The invention relates to a method for preparing lycopene by B. trispora and B. trispora. Background technique [0002] Lycopene, also known as ψ-carotene, is an open-chain unsaturated carotene, which is an intermediate product in the biosynthesis of carotene and lutein, and becomes carotene after two-step cyclization. Its all-trans structure is: [0003] [0004] With the continuous deepening of research, it is found that lycopene has a strong ability to scavenge oxygen free radicals, especially in recent years, many studies have shown that it has significant effects in anti-cancer and anti-cancer. The application of lycopene has also gradually expanded from being only added as a pigment to food, beverages and cosmetics to health care products and pharmaceutical industries. The supply of lycopene is in short supply in domestic and foreign markets. These factors make the production and preparation technology and pathological research of lycopene a ...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12P5/02C12R1/645
CPCC12N1/14C12P5/026C12N1/145C12R2001/645
Inventor 张春颖
Owner 西藏天虹科技股份有限责任公司
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