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Method for producing carotenoid through solid fermentation of blakeslea trispora

A technology of B. trispora and solid fermentation, which is applied to the fields of animal feed and solid fermentation extract, can solve the problems of being unsuitable for application in price sensitivity, high price of beta-carotene, etc., and achieves good dyeing effect and improved Immunity, improve egg production rate

Inactive Publication Date: 2020-04-10
CABIO BIOTECH WUHAN CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This makes the carotenoids from this source, including β-carotene and lycopene crystals, expensive and unsuitable for use in the price-sensitive feed industry

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0077] 1. Preparation of fermentation medium

[0078] 20 parts by weight of kitchen waste solid waste, 10 parts by weight of kitchen waste oil, 1 part by weight of glucose, 2 parts by weight of yeast powder, 1 part by weight of potassium dihydrogen phosphate, 1 part by weight of magnesium sulfate, 30 parts by weight of wheat bran and 35 parts by weight of water Parts were mixed evenly to obtain the fermentation medium of the present embodiment, the nitrogen content accounted for 10% of the total weight of the medium, and the carbon-nitrogen ratio was 6:1.

[0079] 2. Production of carotenoids by solid fermentation of B. trispora

[0080]1) Slant culture: B. trispora positive bacteria and B. trispora negative bacteria were inoculated respectively on the slope containing PDA medium, and cultured at 28°C for 6 days.

[0081] 2) Seed culture: the B. trispora positive bacteria and the B. trispora negative bacteria cultured on the slant were inoculated into the seed culture medium ...

Embodiment 2

[0086] Example 2 The solid fermentation extract of B. trispora was prepared under the same preparation conditions as in Example 1, the only difference being that the fermentation medium in Example 2 contained 17 parts by weight of kitchen waste solid waste and 10 parts by weight of kitchen waste oil , 1 weight part of glucose, 2 weight parts of yeast powder, 1 weight part of potassium dihydrogen phosphate, 1 weight part of magnesium sulfate, 30 weight parts of wheat bran and 38 weight parts of water, its nitrogen content accounts for 8% of the total weight of the culture medium, carbon The nitrogen ratio is 6:1. Example 2 Add blocking agent when fermenting and cultivating for 52h. Sampling, washing and separation of bacteria after the end of fermentation, drying at 105°C to a constant weight to determine that the dry weight of the biomass reaches 82g / kg of material.

[0087] The fermented solid extract prepared in Example 2 contains 57.2% protein, 16.6% fiber, 2.8% β-carotene...

Embodiment 3

[0089] Example 3 The solid fermentation extract of B. trispora was prepared under the same preparation conditions as in Example 1, the only difference being that the fermentation medium in Example 3 contained 15 parts by weight of kitchen waste solid waste and 12 parts by weight of kitchen waste oil , 2 parts by weight of glucose, 3 parts by weight of yeast powder, 1 part by weight of potassium dihydrogen phosphate, 1 part by weight of magnesium sulfate, 28 parts by weight of wheat bran and 38 parts by weight of water, its nitrogen content accounts for 6% of the total weight of the culture medium, carbon The nitrogen ratio is 8:1. Sampling after the end of fermentation, washing and separating the bacteria, drying at 105°C to a constant weight to determine that the dry weight of the biomass can reach 79g / kg material.

[0090] The solid fermented extract prepared in Example 3 contains 58.3% protein, 14.9% crude fiber, 4.2% β-carotene, 2.6% lycopene, 3.8% moisture and 4.0% ash in...

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Abstract

The invention relates to a method for producing carotenoid through solid fermentation of blakeslea trispora, a solid fermentation extract and animal feed. The method comprises the following steps: inoculating blakeslea trispora on a fermentation culture medium and carrying out fermentation culture; and to be specific, the fermentation culture medium contains kitchen solid waste accounting for 5-30% of the total weight of the culture medium and kitchen waste grease accounting for 5-15% of the total weight of the culture medium. The preparation method of the kitchen waste solid waste and the kitchen waste grease comprises the following steps: (1), roughly selecting the kitchen waste; (2), filtering or centrifugally separating the roughly selected kitchen waste to obtain the kitchen solid waste and the kitchen liquid waste; and (3) further carrying out oil-water separation on the kitchen liquid waste to obtain the kitchen waste grease and the kitchen waste swill. Kitchen waste is used asa fermentation medium, so that the cost is low; the kitchen waste is used as a main raw material and carotenoid is generated through biotransformation, so that kitchen waste pollution is reduced whilethe feed application requirement is met.

Description

technical field [0001] The invention relates to the technical field of microbial fermentation, in particular to a method for producing carotenoids by solid fermentation of B. trispora, a solid fermentation extract and animal feed. Background technique [0002] Existing B. trispora fermentation methods usually use liquid fermentation to produce high levels of carotenoids, especially β-carotene, and are characterized by a highly controlled fermentation process, and the resulting material is used to purify carotenoid crystals. This makes the carotenoids from this source, including β-carotene and lycopene crystals, expensive and unsuitable for use in the feed industry with high price sensitivity. [0003] Carotenoids have the characteristics of safety and high coloring degree in function, and are widely demanded in aquaculture and layer poultry farming industries. [0004] Therefore be necessary to provide a kind of new fermentation method of three brazil enzyme fermentation pr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P23/00C12P5/02A23K50/75A23K10/12A23K10/30A23K10/37A23K20/158A23K20/163A23K20/26A23K20/20C12R1/645
CPCA23K10/12A23K10/30A23K10/37A23K20/158A23K20/163A23K20/26A23K20/30A23K50/75C12P5/007C12P23/00Y02P60/87
Inventor 李翔宇刘洋余超陆姝欢汪志明
Owner CABIO BIOTECH WUHAN CO LTD
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