Method for preparing beta-carotene from Blakeslea trispora mycelium

A technology of bollardii and carotene is applied in the fields of medicine, biochemical engineering and food, and can solve the problems of unfavorable industrialized production of efficiency or cost, difficult to guarantee crystal purity and quality, unfavorable solvent recycling and reuse, etc. The effect of reuse, convenient solvent recovery and low solvent residue

Active Publication Date: 2016-12-14
INNOBIO CORP LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the process of adding anti-solvent to promote crystallization is complicated, and the large amount of mixed solvent formed is not conducive to solvent recovery and reuse, and the yield is also low
[0011] The process of preparing high-purity β-carotene crystals from the mycelium of B. trispora as raw materia

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Get 48g of wet mycelium (20g of dry mycelium, 2.41% of dry β-carotene content) as starting material, add 60g of ethyl acetate, stir and wash at room temperature for 30 minutes, filter to obtain 41g of wet mycelium, and then Add 300g (equivalent to 15 times the weight of dry mycelia) ethyl acetate and circulate to shear and break the wall for 15 minutes, extract at 50°C for 30 minutes, filter to obtain the filtrate and residue, then add 300g ( 200g for the first time, 100g for the second time) ethyl acetate, leaching at 50°C for 30 minutes each time, filtering to obtain the filtrate and residue, combining the two filtrates and concentrating to a concentration of β-carotene of about 7000mg / L, Incubate at 2°C for 3 hours, filter to obtain β-carotene crystals, and then vacuum-dry at 40°C for 16 hours to obtain 0.383g of β-carotene crystals, with a product content of 93.1% and a pure product yield of 73.9%. The obtained residue is 10 g, the residue β-carotene content is only...

Embodiment 2

[0053] Get 48g of wet mycelium (20g of dry mycelium, 2.41% of dry beta-carotene content) as starting material, add 60g of ethyl acetate, stir and wash at room temperature for 20 minutes, filter to obtain 39g of wet mycelium, and then Add 260g (equivalent to 13 times the weight of dry mycelia) ethyl acetate and circulate to shear and break the wall for 20 minutes, leach for 40 minutes at 45°C, filter to obtain the filtrate and residue, then add 340g ( 200g for the first time, 140g for the second time) ethyl acetate, each time leaching at 45°C for 40 minutes, filtering to obtain the filtrate and residue, combining the two filtrates and concentrating to about 5400mg / L of β-carotene concentration, Incubate at 5°C for 3.5 hours, filter to obtain β-carotene crystals, and then vacuum-dry at 40°C for 24 hours to obtain 0.398g of β-carotene crystals with a product content of 90.6% and a pure product yield of 74.8%. The obtained residue is 10.8g, the residue β-carotene content is only 0...

Embodiment 3

[0055] Get 49g of wet mycelia (20g of dry mycelium, 2.37% of dry β-carotene content) as starting material, add 70g of ethyl acetate, stir and wash at room temperature for 40 minutes, filter to obtain 40g of wet mycelium, and then Add 300g (equivalent to 15 times the weight of dry mycelia) ethyl acetate and circulate to shear and break the wall for 15 minutes, extract at 50°C for 30 minutes, filter to obtain the filtrate and residue, then add 300g ( 200g for the first time, 100g for the second time) ethyl acetate, leaching at 50°C for 30 minutes each time, filtering to obtain the filtrate and residue, combining the two filtrates and concentrating to a concentration of β-carotene of about 6000mg / L, Incubate at 5°C for 3 hours, filter to obtain β-carotene crystals, and then vacuum-dry at 40°C for 18 hours to obtain 0.406g of β-carotene crystals with a product content of 97% and a pure product yield of 83.1%. The obtained residue is 10.8g, and the residue β-carotene content is onl...

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Abstract

The invention discloses a method for preparing beta-carotene from Blakeslea trispora mycelium. The method comprises extraction and recrystallization process stages. A two-step recrystallization method is adopted in the recrystallization processing stage and comprises the steps that B1, total extracting liquid is concentrated till the concentration of beta-carotene is 5500-6500 mg/L, heat preservation and standing are carried out at the temperature of -5-10 DEG C, and then filtration is carried out; B2, the filtrate obtained in the step B1 is concentrated till the concentration of beta-carotene is 5500-6500 mg/L, heat preservation and standing are carried out at the temperature of 0-15 DEG C, and then filtration is carried out. Mycelium drying is not needed, wet mycelium is directly used for extraction, the process is simple, energy consumption is saved, only single organic solvent is used in the whole process, the production process is easy to control, the cost is low, the high purity and high yield of the product can be ensured at the same time, and the method is suitable for industrial production.

Description

technical field [0001] The invention belongs to the fields of food, medicine and biochemical engineering. More specifically, it relates to a method for extracting and purifying β-carotene from the mycelium of Brassella trispora. Background technique [0002] Carotenoids are a general term for a class of natural pigments, which are commonly found in higher plants, algae and fungi. Although more than 600 natural carotenoids have been isolated at present, there are mainly ten kinds of carotenoid products that have been industrially produced and widely used in food, medicine, feed and other fields, including β-carotene, lutein ( Lutein esters), astaxanthin, lycopene, zeaxanthin, capsanthin, annatto, canthaxanthin, beta-carotene aldehydes and their esters. [0003] As the precursor of vitamin A, β-carotene has been identified as a class A complete nutritional food fortifier by the United Nations Food and Agriculture Organization and the World Health Organization Joint Expert Co...

Claims

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Application Information

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IPC IPC(8): C07C403/24C12P23/00C12R1/645
CPCC07B2200/13C07C403/24C12P23/00
Inventor 范超吴文忠李倩张聚成
Owner INNOBIO CORP LTD
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