Method for producing beta-carotene through fermentation of blakeslea trispora, and beta-carotene

A technology of Blakeslea trispora and carotene, which is applied in the field of fermentation engineering, can solve the problems of reducing the metabolic flux of carotenoids, the decrease of unit production efficiency, and the slowing down of product accumulation rate, so as to improve the yield/cost ratio and simplify Operation, the effect of reducing energy costs

Active Publication Date: 2018-07-31
HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Use KH 2 PO 4 or K 2 HPO 4 Contain PO 4 3+ If the amount of salt is too small, the phosphorylation level will be insufficient, which will greatly reduce the metabolic flux of carotenoids, and if it is too much, it will bring a large amount of K + , so that the cell membrane permeability of the bacteria becomes too strong, and the β-carotene is easily transported from the intracellular to the extracellular, so

Method used

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  • Method for producing beta-carotene through fermentation of blakeslea trispora, and beta-carotene
  • Method for producing beta-carotene through fermentation of blakeslea trispora, and beta-carotene

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Embodiment 1

[0046] The primordial B. trispora positive and the primordial B. trispora minus were respectively inoculated on the slope containing the PDA medium, cultured at 28°C for 6 days, and then transferred to the seed medium at 28°C, Cultivate for 48 hours at 220 rpm, and inject air at a flow rate of 1.5 vvm during the cultivation process.

[0047] The cultured B. trispora positive bacteria and B. trispora negative bacteria were inoculated into a fermenter containing a fermentation medium at a weight ratio of 1:5. At the time of inoculation, the dry weight of the biomass of B. trispora positive and B. trispora negative was 15g / L.

[0048] At the same time, after inoculation, dry compressed air was introduced into the fermentor at a flow rate of 1.5 vvm, and then fermented at a stirring speed of 200 rpm. During the fermentation process, the biomass dry weight of the fermentation system can reach about 45g / L. Phytic acid was added during fermentation to control the pH of the fermentation ...

Embodiment 2

[0053] B. trispora trispora positive and negative B. trispora trispora were respectively inoculated on a slant containing PDA medium, cultured at 26°C for 8 days, and then transferred to seed medium at 26°C, Cultivate for 52 hours at 200 rpm, and inject air at a flow rate of 1 vvm during the cultivation process.

[0054] The cultured B. trispora positive bacteria and B. trispora negative bacteria were inoculated into a fermenter containing a fermentation medium at a weight ratio of 1:3. At the time of inoculation, the dry weight of the biomass of B. trispora positive and B. trispora negative was 12g / L.

[0055] At the same time, after inoculation, dry compressed air was introduced into the fermentor at a flow rate of 1 vvm, and then fermented at a stirring speed of 180 rpm. During the fermentation process, the biomass dry weight of the fermentation system was 40 g / L. Phytic acid was added during the fermentation process to control the pH of the fermentation system to 6.2.

[0056]...

Embodiment 3

[0060] The primordial B. trispora positive and the primordial B. trispora minus were respectively inoculated on the slope containing the PDA medium, cultured at 30°C for 4 days, and then transferred to the seed medium at 30°C, Cultivation was carried out at 240 rpm for 44 hours, and air was introduced at a flow rate of 2 vvm during the cultivation process.

[0061] The cultured B. trispora and B. trispora negative bacteria were inoculated into a fermenter containing fermentation medium at a weight ratio of 1:7. The dry weight of the biomass of Laminella spp. is 10g / L.

[0062] At the same time, after inoculation, dry compressed air was introduced into the fermentor at a flow rate of 2vvm, and then fermented at a stirring speed of 220rpm. During the fermentation process, the biomass dry weight of the fermentation system was 50g / L. Phytic acid was added during fermentation to control the pH of the fermentation system to 6.0.

[0063] When the fermentation is 60h, the feeding amount ...

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Abstract

The invention relates to a method for producing beta-carotene through fermentation of blakeslea trispora, and the beta-carotene, and belongs to the field of fermentation engineering. The method comprises the steps of respectively culturing blakeslea trispora positive bacteria and blakeslea trispora negative bacteria, simultaneously inoculating, fermenting, controlling a pH value of a fermentationsystem to be 5.8 to 6.2 through a modifier during a fermenting process, semi-continuously culturing, emptying when the fermenting starts for 60h, and supplementing materials. The modifier is an acidicmaterial which contains PO4<3+> and does not contain metal ions and salt. A fermentation medium adopted in the fermenting process contains a starch phosphate material. The method is simple and low-cost; through using the acidic material which contains the PO4<3+> and does not contain the metal ions and the salt for controlling a fermenting pH value and providing the PO4<3+>, excessive K<+> is prevented from being brought in; by the cooperative use of the starch phosphate material, the yield of the beta-carotene is improved. Therefore, the obtain beta-carotene is higher in yield, and the method is suitable for industrial production.

Description

Technical field [0001] The invention relates to the field of fermentation engineering, and in particular to a method for producing β-carotene by B. trispora fermented and β-carotene. Background technique [0002] Existing fermentation methods usually use KH 2 PO 4 Or K 2 HPO 4 Etc. containing PO 4 3+ Of salt to increase phosphorylation level, while providing K + , Enhance the permeability of cell membrane, which is beneficial for nutrients to enter cells. Use KH 2 PO 4 Or K 2 HPO 4 Etc. containing PO 4 3+ If the amount of salt is too small, the phosphorylation level is not enough, which greatly reduces the carotenoid metabolic flux, and too much will bring a lot of K + , Making the cell membrane permeability of the bacteria too strong, β-carotene is easily transported from the intracellular to the extracellular, so that the product accumulation rate is correspondingly slow, and the unit production efficiency is reduced. [0003] At the same time, the existing B. trispora fermentati...

Claims

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Application Information

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IPC IPC(8): C12P23/00C12R1/645
CPCC12P23/00
Inventor 李翔宇汪志明陆姝欢余超刘洋姚建铭
Owner HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
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