Method for producing beta-carotene through fermentation of blakeslea trispora, and beta-carotene

Abstract

The invention relates to a method for producing beta-carotene through fermentation of blakeslea trispora, and the beta-carotene, and belongs to the field of fermentation engineering. The method comprises the steps of respectively culturing blakeslea trispora positive bacteria and blakeslea trispora negative bacteria, simultaneously inoculating, fermenting, controlling a pH value of a fermentationsystem to be 5.8 to 6.2 through a modifier during a fermenting process, semi-continuously culturing, emptying when the fermenting starts for 60h, and supplementing materials. The modifier is an acidicmaterial which contains PO4<3+> and does not contain metal ions and salt. A fermentation medium adopted in the fermenting process contains a starch phosphate material. The method is simple and low-cost; through using the acidic material which contains the PO4<3+> and does not contain the metal ions and the salt for controlling a fermenting pH value and providing the PO4<3+>, excessive K<+> is prevented from being brought in; by the cooperative use of the starch phosphate material, the yield of the beta-carotene is improved. Therefore, the obtain beta-carotene is higher in yield, and the method is suitable for industrial production.

Description

Technical field [0001] The invention relates to the field of fermentation engineering, and in particular to a method for producing β-carotene by B. trispora fermented and β-carotene. Background technique [0002] Existing fermentation methods usually use KH 2 PO 4 Or K 2 HPO 4 Etc. containing PO 4 3+ Of salt to increase phosphorylation level, while providing K + , Enhance the permeability of cell membrane, which is beneficial for nutrients to enter cells. Use KH 2 PO 4 Or K 2 HPO 4 Etc. containing PO 4 3+ If the amount of salt is too small, the phosphorylation level is not enough, which greatly reduces the carotenoid metabolic flux, and too much will bring a lot of K + , Making the cell membrane permeability of the bacteria too strong, β-carotene is easily transported from the intracellular to the extracellular, so that the product accumulation rate is correspondingly slow, and the unit production efficiency is reduced. [0003] At the same time, the existing B. trispora fermentati...

AI Technical Summary

Problems solved by technology

Use KH 2 PO 4 or K 2 HPO 4 Contain PO 4 3+ If the amount of salt is too small, the phosphorylation level will be insufficient, which will greatly reduce the metabolic flux of carotenoids, and if it is too much, it will bring a large amount of K + , so that the cell membrane permeability of the bacteria becomes too strong, and the β-carotene is easily transported from the intracellular to the extracellular, so that the accumulation rate of the product is correspondingly slowed down, and the unit production efficiency is reduced.

[0003] At the same time, the existing B. trispora fermentation of β-carotene is mainly based on single-tank fermentation. In view of its limited natural output and long fermentation cycle, it is necessary to develop a semi-continuous or continuous culture process to further reduce production. cost

Images