Microcapsule and fatty powder containing beta-carotene

A technology of carotene and microcapsules, applied in the direction of microorganisms, microorganisms, food forming, etc., can solve the problem of low production of β-carotene, and achieve the effect of simple fermentation process, high yield and easy control

Active Publication Date: 2016-09-07
CABIO BIOTECH WUHAN CO LTD
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  • Application Information

AI Technical Summary

Problems solved by technology

However, the yield of β-carotene produced by using this strain is low, and the highest yield does not exceed 1.3g / L

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0009] In order to understand the technical content of the present invention more clearly, the following examples are given in detail.

[0010] Breeding method of mutant strain of B. trispora

[0011] 1) Commercially available B. trispora positive bacteria (preservation number: CCTCCAF97006(+)) and B. trispora negative bacteria (preservation number: CCTCCAF96002(-)) were used as starting strains.

[0012] 2) The two starting strains were cultured for 5 days until the spores matured.

[0013] 3) Filter through gauze or filter paper to obtain pure spore liquid, inject the spore liquid into a sterile petri dish, and dry it with sterile air to become bacterial plaque. Aseptically move the petri dish containing bacterial plaque into a high-energy particle beam implanter, and undergo mutagenesis by high-energy ion beam implantation.

[0014] 4) The bacterial plaques of the positive and negative strains were eluted with sterile water respectively, and the obtained positive and neg...

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Abstract

The invention provides a microcapsule or fatty powder containing beta-carotene. The beta-carotene is obtained through fermentation of Blakeslea trispora mutant strains. The Blakeslea trispora mutant strains are characterized in that the strains are preserved in China Center for Type Culture Collection (CCTCC), Wuhan University, Wuhan, Hubei Province, China on August 8th, 2014 with accession numbers of Blakeslea trispora BT7251 (+) CCTCC M 2014378 and Blakeslea trispora BT7603 (-) CCTCC M 2014379. After the Blakeslea trispora mutant strains provided by the invention are subjected to conventional fermentation culture, high-yield beta-carotene can be obtained. Moreover, the whole fermentation process is simple, convenient to control and beneficial for industrial production.

Description

technical field [0001] The invention relates to a microcapsule containing beta-carotene and fat powder. Background technique [0002] β-carotene is one of more than 500 kinds of carotene. It is the precursor of vitamin A, also known as provitamin A. Its dilute solution is orange-yellow to yellow. β-carotene widely exists in plants, algae and fungi, but animals and humans cannot synthesize carotene and must be ingested from the outside. As a food additive, β-carotene can act as a pigment and a nutritional enhancer. At the same time, β-carotene is a powerful antioxidant, which can inhibit the proliferation of cancer cells and improve the body's immunity. The unique nutritional value and medicinal efficacy make carotene highly valued by medical circles and food merchants all over the world. [0003] At present, the preparation methods of β-carotene mainly include the following types: 1. Chemical synthesis, that is, using organic chemical raw materials to artificially synthesi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P23/00A23L33/10A23P10/30C12R1/645
CPCA23V2002/00C12P23/00C12N1/145C12R2001/645A23V2250/211
Inventor 汪志明李翔宇余超陆姝欢周强肖敏张玉良
Owner CABIO BIOTECH WUHAN CO LTD
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