Method for improving beta-carotene produced from blakeslea trispora by virtue of organic acid

A technology of Brassella trispora and Brassella, applied in the field of β-carotene, can solve the problems of limited development, toxic and side effects, low product absorption rate, etc., and the method is simple, easy to implement, low in cost, and high in content Effect

Inactive Publication Date: 2015-04-29
XIAMEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The natural extraction method requires rich sources of raw materials, but the pigment content is too low (Shi Bin. Using Dunaliella salina to extract β-carotene [J]. Fine Chemical Industry, 1994,11(5):17-19), and is affected by the climate , origin, transportation and other conditions are limited, and the production cost remains high, making it difficult to produce β-carotene on a

Method used

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  • Method for improving beta-carotene produced from blakeslea trispora by virtue of organic acid
  • Method for improving beta-carotene produced from blakeslea trispora by virtue of organic acid
  • Method for improving beta-carotene produced from blakeslea trispora by virtue of organic acid

Examples

Experimental program
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Effect test

Embodiment 1

[0031] 1) Incline culture: Prepare corn flour agar medium (corn flour 30g / L, malt flour 10g / L, agar powder 15g / L, pH value 7.2). Open the frozen glycerol tube, and take 15 μL of Blakeslea trispora (+) (purchased from the American Type Culture Collection (ATCC), number ATCC 14271 (+)), (-) (purchased from the U.S. Typical strain collection center (ATCC), the number is ATCC 14272 (-)) bacteria liquid, spread on corn flour agar medium, and cultivate at 28°C for 120h.

[0032] 2) Preparation of seed medium: Dissolve 47g of corn flour and 23g of soybean flour in 1000mL of deionized water, and gelatinize at 100°C for 20min. After cooling, add 2g of potassium dihydrogen phosphate, 0.5g of magnesium sulfate, 0.05g of anhydrous calcium chloride, and 0.09g of ferrous sulfate heptahydrate. After stirring and mixing, adjust the pH value to 6.2 with 40% sodium hydroxide solution, add deionized water to make up to 1000mL, dispense into 250mL Erlenmeyer flasks, and sterilize in an autoclave...

Embodiment 2

[0047] 1) Incline culture: Prepare corn flour agar medium (corn flour 30g / L, malt flour 10g / L, agar powder 15g / L, pH value 6.2). Open the frozen glycerol tube, and take 15 μL of Blakeslea trispora (+) (purchased from the American Type Culture Collection (ATCC), number ATCC 14271 (+)), (-) (purchased from the U.S. Typical strain collection center (ATCC), the number is ATCC 14272 (-)) bacteria liquid, spread on corn flour agar medium, and cultivate at 28°C for 120h.

[0048] 2) Preparation of seed medium: Dissolve 47g of corn flour and 23g of soybean flour in 1000mL of deionized water, and gelatinize at 100°C for 20 minutes. After cooling, add 2g potassium dihydrogen phosphate, 0.5g magnesium sulfate, 0.05g anhydrous calcium chloride, 0.09g ferrous sulfate heptahydrate, stir and mix well, adjust the pH value to 6.2 with 40% sodium hydroxide solution, add deionized Dilute water to 1000mL, dispense into 250mL Erlenmeyer flasks, and sterilize in a high-pressure steam sterilizer at...

Embodiment 3

[0062]1) Incline culture: Prepare corn flour agar medium (corn flour 30g / L, malt flour 10g / L, agar powder 15g / L, pH value 6.2). Open the frozen glycerol tube, and take 15 μL of Blakeslea trispora (+) (purchased from the American Type Culture Collection (ATCC), number ATCC 14271 (+)), (-) (purchased from the U.S. Typical strain collection center (ATCC), the number is ATCC 14272 (-)) bacteria liquid, spread on corn flour agar medium, and cultivate at 28°C for 120h.

[0063] 2) Preparation of seed medium: Dissolve 47g of corn flour and 23g of soybean flour in 1000mL of deionized water, and gelatinize at 100°C for 20min. After cooling, add 2g of potassium dihydrogen phosphate, 0.5g of magnesium sulfate, 0.05g of anhydrous calcium chloride, and 0.09g of ferrous sulfate heptahydrate. After stirring and mixing, adjust the pH value to 6.2 with 40% sodium hydroxide solution, add deionized water to make up to 1000mL, dispense into 250mL Erlenmeyer flasks, and sterilize in an autoclave ...

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Abstract

The invention discloses a method for improving beta-carotene produced from blakeslea trispora by virtue of organic acid, and relates to beta-carotene. The method comprises the following steps: 1) taking ATCC 14271 (+) and 14272 (-) bacterium liquids from blakeslea trispora, smearing the bacterium liquids on a corn meal agar medium and cultivating; picking two rings of positive and negative bacteria from an ATCC 14271 (+) and 14272 (-) strain slab, and respectively inoculating and cultivating in a conical flask containing a seed medium so as to obtain an ATCC 14271 (+) seed liquid and an ATCC14272 (-) seed liquid; mixing the two seed liquids and inoculating the seed liquids to a conical flask containing a fermenting medium; adding organic acid and cultivating; and after fermenting, collecting bacteria, filtering and cleaning, drying wet bacteria to constant weight so as to obtain the dry weight of cells, and detecting the content of the beta-carotene. The method disclosed by the invention is simple and easy to implement, low in cost and high in beta-carotene content.

Description

technical field [0001] The invention relates to β-carotene, in particular to a method for improving the production of β-carotene by using an organic acid. Background technique [0002] β-Carotene (-Carotene), also known as carotene, is a fat-soluble pigment widely present in the biological world and belongs to tetraterpenoid hydrocarbons (López-Nieto MJ, Costa J, Peiro E, Méndez E, Rodríguez - Sáiz M, de la Fuente JL, Cabri W, Barredo JL. Biotechnological lycopene production by mated fermentation of Blakeslea trispora [J]. Appl Microbio Biotechnol, 2004, 66: 153-159). Because β-carotene itself has the physiological functions of scavenging free radicals and anti-oxidation, it is closely related to human health. It not only has anti-cancer and anti-cancer effects, but also has important significance for the prevention of coronary heart disease and other cardiovascular diseases. Now, β-carotene has been widely used in food additives, health care medicine, cosmetics, etc. :4-5...

Claims

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Application Information

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IPC IPC(8): C12P23/00C12R1/645
Inventor 敬科举何舒雅谢友坪卢英华
Owner XIAMEN UNIV
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