Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for improving beta-carotene produced from blakeslea trispora by virtue of organic acid

A technology of Brassella trispora and Brassella, applied in the field of β-carotene, can solve the problems of limited development, toxic and side effects, low product absorption rate, etc., and the method is simple, easy to implement, low in cost, and high in content Effect

Inactive Publication Date: 2015-04-29
XIAMEN UNIV
View PDF2 Cites 10 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The natural extraction method requires rich sources of raw materials, but the pigment content is too low (Shi Bin. Using Dunaliella salina to extract β-carotene [J]. Fine Chemical Industry, 1994,11(5):17-19), and is affected by the climate , origin, transportation and other conditions are limited, and the production cost remains high, making it difficult to produce β-carotene on a large scale
The β-carotene synthesized by chemical synthesis is relatively cheap and has great market competitiveness, but its structure is all trans, the product absorption rate is low, and certain toxic and side effects are arranged, which limits the development of its market ( Zhan Haoqiang.Industrial Synthesis of β-Carotene[J].Chemical Production and Technology,1997,(2):14-17)

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for improving beta-carotene produced from blakeslea trispora by virtue of organic acid
  • Method for improving beta-carotene produced from blakeslea trispora by virtue of organic acid
  • Method for improving beta-carotene produced from blakeslea trispora by virtue of organic acid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] 1) Incline culture: Prepare corn flour agar medium (corn flour 30g / L, malt flour 10g / L, agar powder 15g / L, pH value 7.2). Open the frozen glycerol tube, and take 15 μL of Blakeslea trispora (+) (purchased from the American Type Culture Collection (ATCC), number ATCC 14271 (+)), (-) (purchased from the U.S. Typical strain collection center (ATCC), the number is ATCC 14272 (-)) bacteria liquid, spread on corn flour agar medium, and cultivate at 28°C for 120h.

[0032] 2) Preparation of seed medium: Dissolve 47g of corn flour and 23g of soybean flour in 1000mL of deionized water, and gelatinize at 100°C for 20min. After cooling, add 2g of potassium dihydrogen phosphate, 0.5g of magnesium sulfate, 0.05g of anhydrous calcium chloride, and 0.09g of ferrous sulfate heptahydrate. After stirring and mixing, adjust the pH value to 6.2 with 40% sodium hydroxide solution, add deionized water to make up to 1000mL, dispense into 250mL Erlenmeyer flasks, and sterilize in an autoclave...

Embodiment 2

[0047] 1) Incline culture: Prepare corn flour agar medium (corn flour 30g / L, malt flour 10g / L, agar powder 15g / L, pH value 6.2). Open the frozen glycerol tube, and take 15 μL of Blakeslea trispora (+) (purchased from the American Type Culture Collection (ATCC), number ATCC 14271 (+)), (-) (purchased from the U.S. Typical strain collection center (ATCC), the number is ATCC 14272 (-)) bacteria liquid, spread on corn flour agar medium, and cultivate at 28°C for 120h.

[0048] 2) Preparation of seed medium: Dissolve 47g of corn flour and 23g of soybean flour in 1000mL of deionized water, and gelatinize at 100°C for 20 minutes. After cooling, add 2g potassium dihydrogen phosphate, 0.5g magnesium sulfate, 0.05g anhydrous calcium chloride, 0.09g ferrous sulfate heptahydrate, stir and mix well, adjust the pH value to 6.2 with 40% sodium hydroxide solution, add deionized Dilute water to 1000mL, dispense into 250mL Erlenmeyer flasks, and sterilize in a high-pressure steam sterilizer at...

Embodiment 3

[0062]1) Incline culture: Prepare corn flour agar medium (corn flour 30g / L, malt flour 10g / L, agar powder 15g / L, pH value 6.2). Open the frozen glycerol tube, and take 15 μL of Blakeslea trispora (+) (purchased from the American Type Culture Collection (ATCC), number ATCC 14271 (+)), (-) (purchased from the U.S. Typical strain collection center (ATCC), the number is ATCC 14272 (-)) bacteria liquid, spread on corn flour agar medium, and cultivate at 28°C for 120h.

[0063] 2) Preparation of seed medium: Dissolve 47g of corn flour and 23g of soybean flour in 1000mL of deionized water, and gelatinize at 100°C for 20min. After cooling, add 2g of potassium dihydrogen phosphate, 0.5g of magnesium sulfate, 0.05g of anhydrous calcium chloride, and 0.09g of ferrous sulfate heptahydrate. After stirring and mixing, adjust the pH value to 6.2 with 40% sodium hydroxide solution, add deionized water to make up to 1000mL, dispense into 250mL Erlenmeyer flasks, and sterilize in an autoclave ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for improving beta-carotene produced from blakeslea trispora by virtue of organic acid, and relates to beta-carotene. The method comprises the following steps: 1) taking ATCC 14271 (+) and 14272 (-) bacterium liquids from blakeslea trispora, smearing the bacterium liquids on a corn meal agar medium and cultivating; picking two rings of positive and negative bacteria from an ATCC 14271 (+) and 14272 (-) strain slab, and respectively inoculating and cultivating in a conical flask containing a seed medium so as to obtain an ATCC 14271 (+) seed liquid and an ATCC14272 (-) seed liquid; mixing the two seed liquids and inoculating the seed liquids to a conical flask containing a fermenting medium; adding organic acid and cultivating; and after fermenting, collecting bacteria, filtering and cleaning, drying wet bacteria to constant weight so as to obtain the dry weight of cells, and detecting the content of the beta-carotene. The method disclosed by the invention is simple and easy to implement, low in cost and high in beta-carotene content.

Description

technical field [0001] The invention relates to β-carotene, in particular to a method for improving the production of β-carotene by using an organic acid. Background technique [0002] β-Carotene (-Carotene), also known as carotene, is a fat-soluble pigment widely present in the biological world and belongs to tetraterpenoid hydrocarbons (López-Nieto MJ, Costa J, Peiro E, Méndez E, Rodríguez - Sáiz M, de la Fuente JL, Cabri W, Barredo JL. Biotechnological lycopene production by mated fermentation of Blakeslea trispora [J]. Appl Microbio Biotechnol, 2004, 66: 153-159). Because β-carotene itself has the physiological functions of scavenging free radicals and anti-oxidation, it is closely related to human health. It not only has anti-cancer and anti-cancer effects, but also has important significance for the prevention of coronary heart disease and other cardiovascular diseases. Now, β-carotene has been widely used in food additives, health care medicine, cosmetics, etc. :4-5...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12P23/00C12R1/645
Inventor 敬科举何舒雅谢友坪卢英华
Owner XIAMEN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com