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Method for preparing beta-carotin from Blakeslea trispora fermentation broth

A technology of carotene and Blarilla mold, which is applied in the fields of food, chemical industry and medicine, can solve the problems of increasing the difficulty of β-carotene, and achieve the effect of easy control of the production process, short process flow and few operation steps

Active Publication Date: 2010-10-27
ZHEJIANG MEDICINE CO LTD XINCHANG PHAMACEUTICAL FACTORY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The patent uses wall-breaking technology, so the subsequent step of solid-liquid separation increases the difficulty of extracting β-carotene by this method

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Use the fermentation broth of Blakeslea trispora to obtain wet mycelium through plate and frame filtration. Take 100g of the wet mycelium and dry it under vacuum at 30°C for 30 hours. The dry mycelium weighs 40g and the moisture content is 9.5%. After grinding through a 40-mesh sieve, the mycelium was leached with 800ml (20 times the weight of mycelium) in dichloromethane at 32°C for 2 hours. The extract was concentrated to a β-carotene concentration of 50000μg / ml, and then kept at -5°C for 3 3.51g of β-carotene crystals obtained by filtration, dissolved in 17.6ml (5 times wet crude) of dichloromethane at 36°C, filtered and concentrated to a β-carotene concentration of 50000μg / ml, incubated at -5°C for 3 hours, and filtered to obtain β-carotene crystals were vacuum dried at 30°C for 16 hours to obtain 1.12 g β-carotene crystals, with a product content of 98.5%.

Embodiment 2

[0028] Take 100g of wet mycelium and dry it in vacuum at 50℃ for 20 hours. The dry hyphae weighs 39g and the moisture content is 7.2%. After grinding through a 40-mesh sieve, 585ml (15 times the mycelium weight) of dichloromethane is extracted at 36℃ for 1 hour. After the extract was concentrated to a β-carotene concentration of 50000μg / ml, it was kept at 15°C for 6 hours, filtered to obtain 3.54g β-carotene crystals, dissolved in 35.4ml (10 times wet crude) in dichloromethane at 32°C, filtered and concentrated Incubate at 15°C for 6 hours to a β-carotene concentration of 50000μg / ml, filter to obtain β-carotene crystals, and then vacuum-dry at 45°C for 8 hours to obtain 1.14g β-carotene crystals with a content of 98.8%.

Embodiment 3

[0030] Take 100g of wet mycelium and dry it under vacuum at 40°C for 25 hours. The dry mycelium weighs 39.2g and the moisture content is 7.3%. After grinding through a 40-mesh sieve, 686ml (17.5 times the mycelium body weight) is extracted with dichloromethane at 34°C for 1.5 hours. After the extract is concentrated to a β-carotene concentration of 50000μg / ml, it is incubated at 5°C for 4.5 hours, filtered to obtain 3.56g β-carotene crystals, dissolved in 26.7ml (7.5 times wet crude) in dichloromethane at 34°C and filtered It was concentrated to a β-carotene concentration of 50000μg / ml and incubated at 5°C for 4.5 hours, filtered to obtain β-carotene crystals, and then vacuum dried at 38°C for 14 hours to obtain 1.15g β-carotene crystals with a content of 99.1%.

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Abstract

The invention relates to a method for preparing beta-carotin from Blakeslea trispora fermentation broth, which comprises the following steps of: filtering Blakeslea trispora fermentation broth to obtain wet mycelia; vacuum-drying the wet mycelia to obtain dry mycelia of which the water content is less than 10%; milling and pulverizing the dry mycelia; screening, leaching the dry mycelia with dichloromethane which is 15-20 times of the dry mycelia by mass, evaporating to concentrate the leaching liquor until the concentration of beta-carotin is not lower than 50000 mu g / ml, keeping the temperature, and filtering to obtain the wet crude product of beta-carotin crystals; and dissolving the wet crude product with dichloromethane, filtering, evaporating to concentrate until the concentration of the beta-carotin is not lower than 50000 mu g / ml, keeping the temperature, filtering, and vacuum-drying to obtain the beta-carotin crystals. The method has the advantages of short technical process,less operation steps, easy control on production process, high product content, low cost, high yield, only one solvent used in the overall process and suitability for industrial production.

Description

Technical field [0001] The invention belongs to the fields of food, medicine, and chemical industry, and relates to the extraction technology of microbial fermentation broth, in particular to a method for preparing β-carotene from the fermentation broth of B. trispora. Background technique [0002] Carotenoids (including β-carotene, lycopene, lutein, etc.) have a variety of important physiological functions such as enhancing immunity and anti-oxidation. Among them, β-carotene is a widespread fat-soluble carotenoid It is closely related to human health. It not only has high medicinal value such as anti-cancer, anti-oxidation and anti-radiation, but also can be converted into vitamin A in humans and animals. It is an important source of essential vitamin A for humans. A large number of data indicate that the low content of β-carotene in human plasma is closely related to the high incidence of lung cancer, and is negatively related to the incidence of breast cancer, stomach cancer, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C403/24
Inventor 邵东吴亚铭
Owner ZHEJIANG MEDICINE CO LTD XINCHANG PHAMACEUTICAL FACTORY
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