Fermentation method for preparing beta-carotin by using Blakeslea trispora
A technology of B. trispora and B. boulardii is applied in the field of fermentation for preparing beta-carotene, can solve the problems such as lack of comprehensive consideration of the fermentation process, unfavorable full utilization of fermenters, influence on the yield of target products and the like, and achieves low cost , Conducive to bacterial growth and product synthesis, the effect of reducing the amount of feed
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Embodiment 1
[0025] Embodiment 1: a kind of fermentation method utilizing B. trispora to prepare β-carotene, it comprises the following steps: (1) strain activation; (2) primary seed cultivation; (3) secondary seed cultivation; ( 4) fermentation culture; wherein,
[0026] (1) Strain activation: under aseptic conditions, inoculate the positive and negative bacterial strains of B. trispora on the slanted PDA medium (potato dextrose agar medium, suitable for fungal growth), and place them in the incubator. Cultivate at 26°C for 4 days, after the positive and negative strains of B. trispora grow spores respectively, scrape and wash the spores in the slant medium with sterile saline under aseptic conditions, and prepare a uniform spore suspension. The concentrations of positive and negative bacteria spore suspensions are respectively: positive bacteria 10 4 spores / mL, negative bacteria 10 5 spores / mL; wherein, Blakesleatrispora positive and negative strains were purchased from ATCC, numbered ...
Embodiment 2
[0033] Embodiment 2: a kind of fermentation method utilizing B. trispora to prepare β-carotene, which comprises the following steps: (1) strain activation; (2) primary seed cultivation; (3) secondary seed cultivation; ( 4) fermentation culture; wherein,
[0034] (1) Strain activation: under aseptic conditions, inoculate the positive and negative bacterial strains of B. trispora on the slanted PDA medium (potato dextrose agar medium, suitable for fungal growth), and place them in the incubator. Cultivate at 30°C for 5 days, after the positive and negative strains of B. trispora grow spores respectively, scrape and wash the spores in the slant medium with sterile saline under aseptic conditions, and prepare a uniform spore suspension. The concentrations of positive and negative bacteria spore suspensions are respectively: positive bacteria 10 4 spores / mL, negative bacteria 10 5 spores / mL; wherein, Blakesleatrispora positive and negative strains were purchased from ATCC, number...
Embodiment 3
[0041] Embodiment 3: a kind of fermentation method utilizing B. trispora to prepare β-carotene, it comprises the following steps: (1) strain activation; (2) primary seed cultivation; (3) secondary seed cultivation; ( 4) fermentation culture; wherein,
[0042] (1) Strain activation: under aseptic conditions, inoculate the positive and negative bacterial strains of B. trispora on the slanted PDA medium (potato dextrose agar medium, suitable for fungal growth), and place them in the incubator. Cultivate at 28°C for 4 days, after the positive and negative strains of B. trispora grow spores respectively, scrape and wash the spores in the slant medium with sterile saline under aseptic conditions, and prepare a uniform spore suspension. The concentrations of positive and negative bacteria spore suspensions are respectively: positive bacteria 10 4spores / mL, negative bacteria 10 5 spores / mL; wherein, Blakesleatrispora positive and negative strains were purchased from ATCC, numbered A...
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