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Method for extracting and separating isoflavone from kudzu

A technology for isoflavones and extracts, applied in the field of extraction and separation of isoflavones, can solve the problems of low content of puerarin products, low extraction efficiency, long operation cycle, etc., and achieves great ecological environment effect, significant social benefit, and short extraction cycle. Effect

Inactive Publication Date: 2006-05-10
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main problems of these processes are that the operation cycle is long, the extraction efficiency is not high, the content of the puerarin product obtained is generally low, and organic solvents such as n-butanol and ethyl acetate are used in the extraction process, which leads to the difficulty of solving the solvent residue problem.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Take by weighing 2 kg of dried kudzu vine, pulverize kudzu vine twice coarsely and finely to obtain a powder of 60 to 100 mesh, and at an extraction temperature of 60° C., use 95% ethanol as an extraction solvent, and the solid-to-liquid ratio of each extraction is 1: 2.5, extract 3 times, the total extraction time is 4 hours, respectively 2 hours for the first time, 1 hour for the second time and 1 hour for the third time. The extracts were combined, filtered and concentrated under reduced pressure to obtain the total isoflavones with an extraction rate of 90%.

[0025] Put the total isoflavones on a 30-60-mesh polyamide column, and elute with gradients of water and 25-95% ethanol. First, it was eluted with water and tracked by TLC. After a fraction comes out, change to 25% ethanol (V / V) solution for elution. The concentration of the eluent was gradually increased, and after the puerarin basically eluted, the concentration of the eluent was increased to 45% until it ...

Embodiment 2

[0027] Take by weighing 2 kg of dried kudzu vine, pulverize kudzu vine twice coarsely and finely to obtain a powder of 100 to 150 mesh, and at an extraction temperature of 45° C., use 70% ethanol as the extraction solvent, and the solid-to-liquid ratio of each extraction is 1: 3. Extract 3 times, the total extraction time is 3 hours, and each extraction time is 1 hour. The extracts were combined, filtered and concentrated under reduced pressure to obtain the total isoflavones with an extraction rate of 86%.

[0028] Put the total isoflavones on a 30-60-mesh polyamide column, and elute with a gradient of water and 25-95% ethanol. First, elute with water, and follow up with TLC. After a fraction comes out, change to 25% ethanol (V / V) solution for elution. The concentration of the eluent was gradually increased in turn, and after the puerarin basically eluted, the concentration of the eluent was increased to 55% until it was raised to 95%. Collect the eluate rich in puerarin an...

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Abstract

The invention discloses a process for extracting and separating isoflavones from kudzu vine, which comprises the steps of disintegrating kudzu vine to 60-150 meshes, loading into a liquid dynamic continuous flow upstream extraction device for alcohol extraction, combining the extract liquid and filtering, concentrating the filtrate to obtain total isoflavones extract, and separating the total isoflavones extract to obtain puerarin and soybean aglycone.

Description

(1) Technical field [0001] The invention relates to a method for extracting and separating isoflavones from Pueraria vine. (2) Background technology [0002] Pueraria vine and kudzu root are also rich in isoflavone compounds, the main active ingredients are puerarin, daidzin and daidzein. In recent years, a large number of literatures at home and abroad have reported the pharmacological effects of its active ingredients. Puerarin and daidzin have been used as raw materials to make medicines and are widely used clinically. Puerarin is used to treat angina pectoris, hypertension, myocardial infarction, arrhythmia and hyperviscosity; It has dilation effect on cerebral arteries, and also has important curative effect on inhibiting cancer cells and anti-aging. In particular, at present, puerarin has become one of the first-choice drugs for cardiovascular and cerebrovascular treatment in hospitals at all levels; puerarin isoflavones are mainly used as raw materials for new drugs...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D311/36
Inventor 苏为科李坚军
Owner ZHEJIANG UNIV OF TECH
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