Novel method for effectively extracting lentinan

A high-efficiency technology of lentinan, applied in the field of plant active ingredient extraction, can solve the problems of low extraction rate, large loss of active ingredients, long cycle and the like, and achieves the effects of simple operation, improved extraction efficiency and low cost

Inactive Publication Date: 2013-04-24
胡玮
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these methods all have disadvantages such as long period, low extraction rate, and large loss of active ingredients.

Method used

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  • Novel method for effectively extracting lentinan
  • Novel method for effectively extracting lentinan

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Get the dried shiitake mushroom fruiting bodies, wash and remove visible impurities therein, pulverize with a pulverizer, and pass through a 40-mesh sieve. Take 10 parts by weight of shiitake mushroom powder and place it in a stainless steel extraction tank, add 300 parts by weight of water, rapidly heat up to 160°C, and keep at this temperature for 5 minutes to release the extract from the lower end of the extraction tank, then add 300 parts by weight Weight of water, repeat the above extraction step once. The extracts extracted twice are combined, filtered with a filter cloth after cooling, and then the filtrate is concentrated using an ultrafiltration membrane with a molecular weight cut-off of 50,000 until the weight of the filtrate is 2.0 times that of the mushroom powder. Add 95% ethanol pre-cooled to 4°C in the filtrate, stir evenly, let stand at 4-8°C for 24 hours, then centrifuge at 4000r / min to collect the precipitate, recover ethanol from the supernatant for ...

Embodiment 2

[0037] Take the dried shiitake stalks, wash and remove visible impurities therein, pulverize them with a pulverizer, and pass through a 40-mesh sieve. Take 10 parts by weight of shiitake mushroom powder and place it in a stainless steel extraction tank, add 200 parts by weight of water, rapidly heat up to 160°C, and keep at this temperature for 6 minutes to release the extract from the lower end of the extraction tank, then add 200 parts by weight Weight of water, repeat the above extraction step once. The extracts extracted twice are combined, filtered with a filter cloth after cooling, and then the filtrate is concentrated using an ultrafiltration membrane with a molecular weight cut off of 50,000 until the weight of the filtrate is 1.5 times the weight of the mushroom powder. Add 95% ethanol pre-cooled to 4°C in the filtrate, stir evenly, let stand at 4-8°C for 24 hours, then centrifuge at 4000r / min to collect the precipitate, recover ethanol from the supernatant for recycl...

Embodiment 3

[0040] Get the dried shiitake mushroom fruiting bodies, wash and remove visible impurities therein, pulverize with a pulverizer, and pass through a 40-mesh sieve. Take 10 parts by weight of shiitake mushroom powder and place it in a stainless steel extraction tank, add 350 parts by weight of water, rapidly heat up to 160°C, and keep at this temperature for 6 minutes to release the extract from the lower end of the extraction tank, then add 350 parts by weight Weight of water, repeat the above extraction step once. The extracts extracted twice are combined, filtered with a filter cloth after cooling, and then the filtrate is concentrated using an ultrafiltration membrane with a molecular weight cut off of 50,000 until the weight of the filtrate is 1.5 times the weight of the mushroom powder. Add 80% edible ethanol pre-cooled to 4°C into the filtrate, stir evenly, let stand at 4-8°C for 24 hours, then centrifuge at 4000r / min to collect the precipitate, and recover the ethanol fr...

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Abstract

The invention discloses a novel method for effectively extracting lentinan, wherein lentinus edodes sporophores or lentinus edodes stems are taken as a raw material, a subcritical water extraction technology is adopted, coarse lentinan is obtained after the processes of ultrafiltration concentration, ethanol precipitation and decolorization drying, protein is removed by anion exchange resin, and the purity of the refined lentinan can reach above 90%. By utilizing the method, the extraction period is greatly shortened, the active ingredient activity is maintained to the maximum extent, the extraction efficiency can be obviously increased; and moreover, compared with other extraction methods, the method has the advantages of no toxity, no harm, energy conservation and environmental friendliness as well as wide application prospect.

Description

technical field [0001] The invention relates to the field of extracting effective plant components, in particular to a method for extracting and purifying lentinan. technical background [0002] Mushroom [Lentinus edodes (Berk.) Sing], also known as Xiangtan and thick mushroom, is a fungus of the Basidiomycetes Apiaceae family. It is known as the "king of mountain delicacies". It is a high-protein, Low-fat nutraceutical food. As early as in the "Compendium of Materia Medica", it is recorded that shiitake mushrooms are "flat in nature and sweet in taste, can benefit qi and relieve hunger, cure wind and blood, reduce phlegm and regulate qi, benefit taste and food, and manage urination." [0003] Lentinan (LNT for short) is an important effective medicinal component extracted from Lentinus edodes. It is a D-(1→3) glucose residue as the main chain and (1→6) glucose residue as side chains of dextran. Since 1969, Chihara et al. first extracted and purified lentinan from the fru...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/02
Inventor 胡玮
Owner 胡玮
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