New process for extracting stevioside by utilizing enzyme coupling membrane separation technology
A separation technology, stevioside technology, applied in the field of food additives, can solve the problems of low conversion rate of stevioside, loss of stevioside, low extraction rate, etc.
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[0015] Example 1
[0016] (1) Enzymatic low-temperature extraction: Take a certain amount of stevia leaves, add 0.1% pectinase and 10 times the amount of tap water for extraction at room temperature, until the extract is identified as negative by the Molish reaction, and the extracts are combined.
[0017] (2) Flocculation process: the extract is added with 10% calcium hydroxide of stevia leaves, flocculates at 60°C for 20 minutes, cooled to room temperature, and centrifuged; the filtrate is added with 4% ferric chloride and adjusted to pH 7.0 at 80°C Flocculate for 30 minutes, centrifuge and filter to obtain flocculent liquid.
[0018] (3) Membrane separation process: the filtrate is filtered through an ultrafiltration membrane with a molecular weight cut-off of 50kD to retain the ultrafiltrate with a molecular weight below 50kD.
[0019] (4) Resin combination process: After the ultrafiltrate is separated and enriched by AB-8 type macroporous adsorption resin, it is desorbed by 70% e...
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