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Extracting process for coral fungi polysaccharide

An extraction process, the technology of coral fungus, which is applied in the field of extraction technology of coral fungus polysaccharides, can solve problems such as the blanks in the field of extraction research, and achieve the effects of improving penetration ability, simple operation, and promoting dissolution and diffusion

Inactive Publication Date: 2009-07-08
夏晖
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main medicinal ingredients of coral fungi are polysaccharides. According to data inquiries, the current research field on the extraction of polysaccharides from coral fungi is basically blank.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] 1) Material pretreatment: select 20g of coral fungus fruiting bodies, crush them, pass through a 30-mesh sieve, add 300ml of water to soak for 1 hour, and wait for extraction;

[0033] 2) Hot water leaching: choose a water bath, heat the above-mentioned pretreated extraction material in a water bath at 80°C for 3 hours, filter, separate the solid from the liquid, discard the solid and prepare to concentrate the supernatant;

[0034] 3) Concentration: put the collected supernatant in a rotary evaporator, concentrate under reduced pressure at 70°C, and concentrate to 1 / 4 of the original volume;

[0035] 4) Alcohol precipitation: Stir and mix the concentrated solution and absolute ethanol at a ratio of 1:1. Since the polysaccharide is insoluble in ethanol, a precipitate is formed and precipitates at the base of the container;

[0036] 5) Centrifugation: After the concentrated solution-ethanol mixture was placed in the refrigerator at 1°C for 24 hours, it was centrifuged at...

Embodiment 2

[0040] 1) Material pretreatment: select 20g of coral fungus fruiting bodies, crush them, pass through a 50-mesh sieve, add 400ml of water to soak for 2 hours, and wait for extraction;

[0041] 2) Hot water leaching: choose a water bath, heat the above-mentioned pretreated extraction material in a water bath at a temperature of 95°C for 2 hours, filter, perform solid-liquid separation, and prepare to concentrate the supernatant after discarding the solid;

[0042] 3) Concentration: put the collected supernatant in a rotary evaporator, and concentrate under reduced pressure at 65°C to 1 / 4 of the original volume;

[0043] 4) Alcohol precipitation: Stir and mix the concentrated solution and absolute ethanol at a ratio of 1:1. Since the polysaccharide is insoluble in ethanol, a precipitate is formed and precipitates at the base of the container;

[0044] 5) Centrifugation: After the concentrated solution-ethanol mixture was placed in the refrigerator at 3°C ​​for 18 hours, it was c...

Embodiment 3

[0048] 1) Material pretreatment: select 20g of coral fungus fruiting bodies, crush them, pass through a 60-mesh sieve, add 500ml of water to soak for 4 hours, and wait for extraction;

[0049] 2) Hot water leaching: choose a water bath, heat the above-mentioned pretreated extraction material in a water bath at 100°C for 1 hour, filter, separate solid from liquid, and prepare to concentrate the supernatant after discarding the solid;

[0050] 3) Concentration: put the collected supernatant in a rotary evaporator, concentrate under reduced pressure at 60°C, and concentrate to 1 / 4 of the original volume;

[0051] 4) Alcohol precipitation: Stir and mix the concentrated solution and absolute ethanol at a ratio of 1:1. Since the polysaccharide is insoluble in ethanol, a precipitate is formed and precipitates at the base of the container;

[0052] 5) Centrifugation: After the concentrated solution-ethanol mixture was placed in the refrigerator at 5°C for 12 hours, it was centrifuged ...

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Abstract

The invention discloses an extracting process for a club fungi polysaccharide. The extracting process comprises the following steps: carrying out the pretreatment of raw materials, carrying out the hot water leaching, ultrasonic extraction or microwave extraction, and storing the extract after concentration, alcohol deposition, centrifugation and drying, wherein the hot water leaching method has moderate conditions and simple operation; and the ultrasonic extraction method and the microwave extraction method can save time and improve the extraction efficiency. The extracted club fungi polysaccharide has an inhibition rate of 70 percent for little white rat S-180 and Ehrlich carcinoma, and has the functions of improving the immunity, diminishing inflammation, easing pain, and the like.

Description

technical field [0001] The invention relates to an extraction process of polysaccharides, in particular to an extraction process of coral fungus polysaccharides. Background technique [0002] Coral fungus (Clavaria zollingeri Lév) belongs to the order Aphyllobacteriaceae, Coralbacteriaceae, and coral fungus. It is named for its resemblance to coral. White, crisp. The taste is delicious, the fragrance is delicious, and it has the effects of harmonizing stomach qi, expelling wind, breaking blood and slowing blood. It is often used by folks to treat stomach pain, persistent food indigestion, and gout. Coral bacteria can prevent cramps and tremors in hands and feet, promote body health and delay aging. Regular food can beautify the skin and improve the body's immunity. The inhibitory rate to mouse sarcoma S-180 and Ehrlich carcinoma is 70%. The main medicinal ingredients of coral fungus are polysaccharides. According to the data query, the research field on the extraction of...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61P35/00A61P37/00
Inventor 夏晖苏延友冯艳
Owner 夏晖
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