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Method for extracting hypericin

An extraction method and technology for hypericin, which are applied in the directions of pharmaceutical formulations, medical preparations containing active ingredients, plant raw materials, etc., can solve the problems of long extraction period, low content of hypericin and high toxicity, and achieve extraction Short cycle, simple operation, low toxicity effect

Inactive Publication Date: 2006-10-25
LANZHOU INST OF ANIMAL SCI & VETERINARY PHARMA OF CAAS +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The solvent used in this extraction method is more toxic, the extraction cycle is long, and the content of hypericin in the extract is relatively low

Method used

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  • Method for extracting hypericin
  • Method for extracting hypericin
  • Method for extracting hypericin

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Experimental program
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Effect test

Embodiment 1

[0026] The extraction of embodiment 1, hypericin

[0027] Now carry out the bioextraction of hypericin with the method of the present invention, flow chart sees figure 1 , including the following steps:

[0028] 1. Dried and crushed Hypericum perforatum to 24 meshes, and accurately weighed 2Kg of Hypericum perforatum powder;

[0029] 2. Put the Hypericum perforatum coarse powder into percolation columns, add in batches, remove impurities with ether, and dry in the shade;

[0030] 3. Mix the impurity-removed Hypericum perforatum with 80% (V / V) ethanol in a volume ratio of 1:3, extract at 40°C for 4 hours, collect the extract, filter it with a filter membrane with a pore size of 0.45um, collect and filter solution, repeat this step 15 times, and combine the filtrate;

[0031] 4. Concentrate the filtrate under reduced pressure to a viscous state at 60°C, and recover the concentrate;

[0032] 5. Adsorb the concentrated solution with a macroporous adsorption resin, elute with 8...

Embodiment 2

[0035] The extraction of embodiment 2, hypericin

[0036] Carry out the biological extraction of hypericin now with the method of the present invention, comprise the following steps:

[0037] 1) Dry and pulverize Hypericum perforatum to 24 mesh, and accurately weigh 3Kg of Hypericum perforatum powder;

[0038] 2) Mix the impurity-removed Hypericum perforatum with 90% (V / V) ethanol in a volume ratio of 2:3, extract at 60°C for 3 hours, collect the extract, filter it with a filter membrane with a pore size of 0.45um, collect and filter solution, repeat this step 20 times, and combine the filtrate;

[0039] 3) concentrating the filtrate under reduced pressure at 60°C until viscous, and recovering the concentrate;

[0040] 4) Adsorb the concentrated solution with LSA-5B special adsorption resin, elute with 90% (V / V) ethanol, and collect the rose red eluate;

[0041] 5) Concentrate the eluate under reduced pressure at 60°C until no liquid flows out to obtain hypericin extract. ...

Embodiment 3

[0043] The extraction of embodiment 3, hypericin

[0044] Carry out the biological extraction of hypericin now with the method of the present invention, comprise the following steps:

[0045] 1) Dry and pulverize Hypericum perforatum to 24 meshes, and accurately weigh 2Kg of Hypericum perforatum powder;

[0046] 2) Put the powder of Hypericum perforatum into a percolation column, add it in batches, remove impurities with ether, and dry in the shade;

[0047] 3) Mix the impurity-removed Hypericum perforatum with 98% (V / V) ethanol in a volume ratio of 2:3, extract at 70°C for 2 hours, collect the extract, filter it with a filter membrane with a pore size of 0.45um, collect and filter solution, repeat this step 20 times, and combine the filtrate;

[0048] 4) concentrating the filtrate under reduced pressure to a viscous state at 50°C, and recovering the concentrate;

[0049] 5) Adsorb the concentrated solution with LSA-5B special adsorption resin, elute with 98% (V / V) ethanol,...

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Abstract

This invention discloses hypericin distillation method. It includes following procedures 1. Leaf weeping forsythia is dried and pulverized. 2. The forsythia powder is mixed with organic solvent as volume ratio 1-2 t 3, then they are distilled at 40-70 degree centigrade for 2-4 hours, extracting solution is collected, filtrated and filtration liquor is collected. 3. The filtration liquor is decompressed and concentrated, concentrated solution is recovered. 4. The liquor is concentrated by resin, and then eluted, eluent is collected. 5. The eluent is decompressed and concentrated, and then hypericin extractive is got. In this invention its operation is simple, easy for industrial application; its distillation cost is low. The extracting solution toxity is little, distilling period is short. Hypericin content in extract is high. So this invention takes important part in hypericin organism distillation.

Description

technical field [0001] The present invention relates to the extraction method of compound, especially relates to the extraction method of hypericin. Background technique [0002] Hypericin (hypericin 4, 4, 5, 5, 7, 7-hexahydroxy-2, 2-dimethyl-middle-naphthodianthrone), is the most abundant in Hypericum Perforatum L. Bioactive substance with antiviral, antidepressant and photokinetic activity. [0003] Since the content of hypericin in the whole plant of Hypericum perforatum is only a few ten-thousandths, it can only be extracted and refined by chemical means. At present, the commonly used extraction methods at home and abroad are as follows: [0004] 1. Methanol or ethanol extraction method: After drying and crushing the whole Hypericum perforatum herb, use ethanol or methanol as a solvent, reflux extraction at 80°C for 1-2 hours, repeat the extraction several times, combine the extracts and concentrate to obtain hypericin extract paste. The product obtained by this meth...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C50/36A61K36/634A61P25/24A61P31/12C07C46/10
Inventor 梁剑平崔颖王学红华兰英尚若锋罗永江牛建荣吕嘉文白卫兵赵晓红王选慧祝艳王曙阳谢等龙
Owner LANZHOU INST OF ANIMAL SCI & VETERINARY PHARMA OF CAAS
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