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General flavone of root of lilac daphne, method of extraction, and application

An extraction method and technology of genkwa root, applied in the field of total flavonoids of genkwa root and its extraction, can solve problems such as destruction, achieve broad application prospects, easy industrial application, and low toxicity

Inactive Publication Date: 2007-02-21
XUZHOU NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In modern medicine, there are three main treatment methods for tumors: chemotherapy, radiotherapy and surgery. Among them, chemotherapy is considered the best treatment method, but most of the chemical drugs also have a destructive effect on the normal cells of the body.

Method used

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  • General flavone of root of lilac daphne, method of extraction, and application
  • General flavone of root of lilac daphne, method of extraction, and application
  • General flavone of root of lilac daphne, method of extraction, and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1, Extraction and HPLC Detection of Total Flavonoids from Daphne Genkkin Root

[0029] 1. Extraction of total flavonoids from Daphne genkwa root

[0030] Select Daphne genkwa root medicinal material that meets the standard of the 2005 edition of "Chinese Pharmacopoeia", and extract the total flavonoids of Daphne genkwa root therefrom with the following method, including the following steps:

[0031] 1) Grinding Daphne genkwa root;

[0032] 2) Mix Daphne genkwa root and absolute ethanol at a volume ratio of 1:4, and extract at 60°C for 24 hours;

[0033] 3) centrifuging to remove the precipitate in the extract;

[0034] 4) The extract was passed through the macroporous adsorption resin ADS-17 (purchased from Tianjin University Resin Research Institute), then the resin was washed with deionized water to remove water-soluble impurities, and then the resin was eluted with absolute ethanol to obtain a yellow eluent;

[0035] 5) The eluate was distilled under redu...

Embodiment 2

[0040] Example 2, Extraction and HPLC Detection of Total Flavonoids from Genkwa Root

[0041] 1. Extraction of total flavonoids from Daphne genkwa root

[0042] Select Daphne genkwa root medicinal material that meets the standard of the 2005 edition of "Chinese Pharmacopoeia", and extract the total flavonoids of Daphne genkwa root therefrom with the following method, including the following steps:

[0043] 1) Grinding Daphne genkwa root;

[0044] 2) Mix Daphne genkwa root and 80% ethanol (V / V) at a volume ratio of 1:5, and extract at 70°C for 36 hours;

[0045] 3) centrifuging to remove the precipitate in the extract;

[0046] 4) The extract is passed through the macroporous adsorption resin ADS-17 (purchased from Tianjin University Resin Research Institute), then the resin is washed with deionized water to remove water-soluble impurities, and then the resin is treated with 80% ethanol (V / V) Eluted to obtain a yellow eluent;

[0047] 5) The eluate was distilled under reduc...

Embodiment 3

[0054] Embodiment 3, detect the effect of total flavonoids of Daphne genkwa root on tumor-bearing mice 180 inhibition of cells

[0055] Detect the total flavonoids of Daphne genkwa root extracted in Example 1 on the tumor-bearing S 180 Inhibitory effect of cells, the experimental method is: take 120 mice and divide them into 12 groups, 10 in each group, half male and half male, among which 11 groups of mice are divided into 5 × 10 7 Cell number will be S 180 The cells were inoculated in the axilla of the mouse hindlimb as an experimental animal model. Dosing group (LLC+TFRD): Homogenize the total flavonoids of Daphne genkwa root with distilled water to form a suspension with a defined concentration, at 25, 50 and 75 mg·kg -1 Three doses were administered to 9 groups of model mice by intragastric administration (ig), wherein 3 groups were administered 7 days before tumor inoculation, 3 groups were administered at the same time as tumor inoculation, and 3 groups were administ...

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Abstract

A general flavone of lilac daphne root is prepared from lilac daphne root through breaking, proportionally mixing it with alcohol, extracting at 40-70 deg.C for 12-48 hr, removing deposit, adsorbing by macroreticular resin, water washing, eluting with alcohol, vacuum distilling, passing through silicon gel column, eluting with the mixture of chloroform and methanol, and concentrating. It can be used to prepare the medicines for preventing and treating the sexual tumor.

Description

technical field [0001] The present invention relates to a compound and its extraction method and application, in particular to the total flavonoids of Daphne genkwa root and its extraction method and its application in the preparation of preventive and / or therapeutic anti-tumor, improving body immunity and anti-aging drugs. Background technique [0002] Tumor is a common and frequently-occurring disease, among which malignant tumor is one of the diseases that seriously endanger human health. According to statistics, the number of people who die of malignant tumors in the world reaches 6.9 million every year. Since the 1970s, the incidence of cancer in my country has been on the rise. At present, the number of cancer patients in my country is about 2 million every year, and the number of people who die of cancer exceeds 1.4 million. [0003] Tumor is a new organism formed by the excessive proliferation and abnormal differentiation of local tissue cells under the action of va...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/83A61K31/365A61P35/00A61P37/00
Inventor 郑维发高晓雯
Owner XUZHOU NORMAL UNIVERSITY
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