Method for extracting soybean dietary fibre and soybean protein from soybean residue

A technology of soybean dietary fiber and soybean protein, which is applied in plant protein processing, protein food ingredients, protein composition of vegetable seeds, etc., to achieve the effects of short process, overcoming equipment corrosion, and low cost

Active Publication Date: 2010-01-20
黄山佳龙绿色食品有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] In order to solve the problem of comprehensive extraction and utilization of water-soluble dietary fiber (SDF), water-insoluble dietary fiber (IDF) and soybean protein in bean dregs, a by-product of soybean

Method used

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  • Method for extracting soybean dietary fibre and soybean protein from soybean residue

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[0021] Example 1:

[0022] The method for extracting soybean dietary fiber and soybean protein from soybean dregs includes the following steps:

[0023] step 1

[0024] Dry the fresh bean dregs at 50-60°C to constant weight, pulverize, and pass through an 80-mesh sieve to obtain bean dregs powder;

[0025] Step 2

[0026] Weigh 10 g of bean dregs powder, add 495 mL of water extract and 7.4 mL of cellulase solution (enzyme activity is 0.22 U / mL), fully stir and mix, and put it into an ultrasonic extractor. Ultrasonic extraction was carried out for 20 minutes under the condition of an empty ratio of 1:0.5 to obtain an ultrasonic extraction solution;

[0027] The above aqueous extract contains cellulase with an enzymatic activity of 0.22-3.14 U / mL. The enzymatic activity of cellulase is defined as the hydrolysis of sodium carboxymethylcellulose substrate under the conditions of 37°C and pH 7.0 to generate 1 umol within 1 minute. The amount of glucose enzyme, as a unit of enzy...

Example Embodiment

[0035] Example 2:

[0036] In step 4, citric acid was added to supernatant C to adjust its pH to 4.5.

[0037] Other steps are the same as in Example 1.

Example Embodiment

[0038] Example 3:

[0039] In step 4, add malic acid to supernatant C to adjust its pH to 4.5.

[0040] Other steps are the same as in Example 1.

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Abstract

The invention relates to a method for extracting soybean dietary fibre and soybean protein from soybean residue, solving the problem of comprehensive use of the side product of soybean residue in soybean. The method comprises the following steps: drying, crashing and sieving fresh soybean to obtain soybean powder, adding extracting solution containing cellulase into the soybean powder for synchronous ultrasound extraction to obtain extracting solution; centrifuging the extracting solution to obtain supernatant A and sediment B; depositing, drying and crashing the supernatant A to obtain soluble dietary fibre; dissolving the sediment B by weak base to obtain supernatant C and sediment D; depositing, drying and crashing the supernatant C by food acid to obtain soybean protein; washing, decolouring, drying and crashing the sediment D to obtain insoluble dietary fibre. The invention utilizes ultrasonic and enzymolysis synergy food acid to obtain soybean soluble dietary fibre, soybean insoluble dietary fibre and soybean protein, and has the advantages of short extraction time, high product yield, simple technical process, easy operation control and convenience for large-scale industrial production.

Description

technical field [0001] The invention belongs to the technical field of deep processing of soybeans, resource utilization of by-products and functional food additives, and specifically relates to a method for extracting soybean dietary fiber and soybean protein from bean dregs. Background technique [0002] Dietary fiber (DF) refers to the general term of polysaccharide carbohydrates and lignin that are not digested by the human body, mainly including cellulose, hemicellulose, lignin, etc. Although dietary fiber is difficult to be decomposed and absorbed in the human digestive tract, it is the seventh nutrient for the human body besides the six major nutrients of carbohydrates, fat, protein, vitamins, water, and minerals, which is indispensable for maintaining human health. Dietary fiber can be divided into water-soluble dietary fiber (SDF) and water-insoluble dietary fiber (IDF) according to its different solubility properties. SDF has good water absorption and moisturizing...

Claims

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Application Information

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IPC IPC(8): A23L1/20A23L1/28A23L1/305A23L1/30A23J1/14A23J3/16A23L33/185A23L33/21
Inventor 洪光明罗建平潘利华徐学玲查学强叶中东
Owner 黄山佳龙绿色食品有限公司
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