Method for extracting, separating and purifying formononetin and calycosin from Astragalus mongholicus waste residue

A technology of vervasoflavone and formononetin, applied in the field of astragalus isoflavones, can solve the problems of wasting astragalus resources and the like, and achieve the effects of good application value, short extraction period and low production cost

Inactive Publication Date: 2010-07-14
NORTHEAST FORESTRY UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

During the production of Astragalus injection, a large number of isoflavones were not extracted, which greatly wasted the resources of Astragalus

Method used

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  • Method for extracting, separating and purifying formononetin and calycosin from Astragalus mongholicus waste residue
  • Method for extracting, separating and purifying formononetin and calycosin from Astragalus mongholicus waste residue

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Experimental program
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Effect test

Embodiment 1

[0028] Accurately weigh 1000 g of astragalus waste residue, dissolve 0.1% solid content of mixed enzymes (cellulase, pectinase, protease, xylanase) in 10 times the volume of warm water, and continuously homogenize and extract 3 times, each time for 2 minutes . Put the homogenate into a constant temperature shaker at 30°C for constant temperature enzymolysis for 12 hours. After the enzymolysis solution is suction-filtered, the filter residue is extracted by negative pressure cavitation with 70% ethanol solution, the pressure is 0.05Mpa, and the solid-liquid ratio is 1:10 (g: mL), extracted at room temperature 3 times, 45min each time, combined the enzymolysis solution after suction filtration and the negative pressure cavitation extraction solution, concentrated under reduced pressure to dryness, dissolved in water, and extracted 3 times with an equal volume of ethyl acetate as the extraction solvent. times, and concentrated to dryness under reduced pressure to obtain a solid. ...

Embodiment 2

[0030]Accurately weigh 1000 g of astragalus waste residue, dissolve 0.3% mixed enzymes (hemicellulose, β-glucosidase, laccase, amylase) in 15 times the volume of warm water, and extract 5 times with continuous homogenate, each time 1min. Put the homogenate in a constant temperature shaker at 30°C for constant temperature enzymolysis for 24 hours. After suction filtration of the enzymolysis solution, add 90% ethanol solution to the filter residue for negative pressure cavitation extraction, the pressure is 0.06Mpa, and the solid-liquid ratio is 1:15 (g: mL), extract at room temperature 5 times, each time for 30min, combine the enzymolysis solution after suction filtration and the negative pressure cavitation extraction solution, concentrate under reduced pressure to dryness, dissolve in water, and mix with 1:1.5 (v / v) ethyl acetate The ester was used as the extraction solvent to extract 5 times, and concentrated to dryness under reduced pressure to obtain a solid. After the so...

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Abstract

The invention provides a method for extracting, separating and purifying formononetin and calycosin from Astragalus mongholicus waste residue. The technical scheme adopted in the invention is as follows: taking waste residue after the production and process of astragalus injection as a raw material; adopting a series of original and efficient technologies for extracting, separation and purifying such as homogenate extraction-mixing enzyme induction biotransformation technology, negative pressure cavitation extract technology, liquid-liquid extraction technology, macroporous absorption resin enrichment technology, normal phase silica gel medium pressure column chromatography technology and devitrification at a low temperature, recrystallization technology and the like to obtain the formononetin and calycosin with high purity, wherein the purity thereof can be more than 95%, and the yield is 80-95%. The raw material used in the invention is the waste residue in the industry production, and the process of the method is simple and practicable, has small pollution to the environment; the obtained formononetin and calycosin have low production cost, high purity and yield, good repeatability, high utilization effect of Astragalus mongholicus resources and the like; and the method is suitable to scaled industrial production.

Description

technical field [0001] The present invention relates to a method for extracting, isolating and purifying functional active ingredients astragalus isoflavones from astragalus waste residue, in particular to a method for extracting, separating and purifying formononetin and calycosin from waste residue of astragalus injection produced and processed . Background technique [0002] Astragalus membranaceus (Fisch.) Bunge var. mongholicus (Bunge) Hsiao or Astragalus membranaceus (Fisch.) Bunge dry roots. According to the Pharmacopoeia, Astragalus is sweet in taste and slightly warm in nature. It has the functions of enhancing the body's non-specificity, improving heart function, dilating coronary arteries, diuresis and detumescence, benefiting health and solidification, promoting granulation of sores, antibacterial, antiviral, anti-fatigue, anti-aging, Promote hematopoietic function, protect the liver and other effects. It is often used for qi deficiency and fatigue, lack of fo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P17/06C07D311/36C07D311/40
Inventor 付玉杰祖元刚闫明明罗猛孔羽顾成波陈彩云赵宝山
Owner NORTHEAST FORESTRY UNIVERSITY
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