Probiotics leavening agent suitable for fermenting cake dreg type feed raw materials and preparation method of probiotics leavening agent

A feed raw material and starter technology, which is applied in animal feed, animal feed, application, etc., can solve the problems of mixed strains, single species, and poor effect, and achieve the effects of high number of viable bacteria, increased nutritional value, and improved taste

Inactive Publication Date: 2013-02-20
BEIJING DABEINONG TECH GRP CO LTD +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The strains used in the existing starter cultures vary from good to bad, and the species is single. Most of them are fermented by Aspergillus fungi. Compared with the fermentation of compound st

Method used

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  • Probiotics leavening agent suitable for fermenting cake dreg type feed raw materials and preparation method of probiotics leavening agent
  • Probiotics leavening agent suitable for fermenting cake dreg type feed raw materials and preparation method of probiotics leavening agent
  • Probiotics leavening agent suitable for fermenting cake dreg type feed raw materials and preparation method of probiotics leavening agent

Examples

Experimental program
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Effect test

Example Embodiment

[0025] Example 1 Preparation of Candida tropicalis powder

[0026] Candida tropicalis strain was purchased from China Food and Fermentation Industry Research Institute.

[0027] Seed medium: 1.0% yeast extract, 2.0% peptone, 2.0% glucose.

[0028] Culture conditions: 28°C, 180rpm, 24h.

[0029] Candida tropicalis culture medium: 1.0% yeast extract, 4.0% glucose, 1.0% peptone, 0.2% potassium dihydrogen phosphate, 0.03% magnesium sulfate.

[0030] Culture conditions: 28°C, 150rpm, 72h.

[0031] The obtained fermentation broth was centrifuged in a high-speed refrigerated centrifuge, and the centrifugal conditions: 6000 rpm, 4°C, 10 min. After centrifugation, the supernatant was discarded to obtain bacterial sludge. Mix 10% glycerol and 0.85% normal saline in a volume ratio of 1:1, dilute and stir the bacterium mud and mix well, then add 10% imported skimmed milk powder and mix well. Place it in an ultra-low temperature refrigerator at -80°C, then place it in a vacuum freeze dryer to dry ...

Example Embodiment

[0032] Example 2 Preparation of Bacillus licheniformis powder

[0033] The strain of Bacillus licheniformis was purchased from China Food and Fermentation Industry Research Institute.

[0034] Seed medium: 0.5% beef extract, 1.0% peptone, 0.5% sodium chloride.

[0035] Culture conditions: 37°C, 180rpm, 24h.

[0036] Bacillus licheniformis culture medium: 3.0% brown sugar, 1.0% corn flour, 3.0% soybean meal, 0.25% urea, 0.15% sodium dihydrogen phosphate.

[0037] Culture conditions: 37°C, 180rpm, 24h.

[0038] The obtained fermentation broth was centrifuged with a high-speed refrigerated centrifuge under the conditions of the centrifugation: 5000rpm, 4°C, 10min. After centrifugation, the supernatant was discarded to obtain bacterial sludge. Stir the bacterium slurry with an aqueous solution of 5.0% sucrose, 1.0% sodium glutamate, and 1.0% ascorbic acid, and then add 50% imported skimmed milk powder. Place it in an ultra-low temperature refrigerator at -80°C, then place it in a vacuum f...

Example Embodiment

[0039] Example 3 Preparation of Lactobacillus plantarum powder

[0040] The Lactobacillus plantarum strain was purchased from China Food and Fermentation Industry Research Institute.

[0041] The seed medium is a commercial MRS broth (a special medium for lactic acid bacteria).

[0042] Culture conditions: 37°C, 100rpm, 12h.

[0043] Lactobacillus plantarum fermentation medium: 3.5% peptone, 6.0% beef powder, 0.50% diammonium citrate, 3.5% sucrose, 1.0% yeast extract, 0.4% dipotassium hydrogen phosphate, 0.1% sodium acetate, 0.02% magnesium sulfate, 0.005% manganese sulfate, 0.05% Tween 80.

[0044] Culture conditions: 37°C, 50rpm, 12h.

[0045] The obtained fermentation broth was centrifuged with a high-speed refrigerated centrifuge under the conditions of the centrifugation: 5000rpm, 4°C, 10min. After centrifugation, the supernatant was discarded to obtain bacterial sludge. Stir the bacterium slurry with an aqueous solution of 5.0% sucrose, 1.0% sodium glutamate, and 1.0% ascorbic a...

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Abstract

The invention discloses a probiotics leavening agent suitable for fermenting cake dreg type feed raw materials and a preparation method of the probiotics leavening agent. The leavening agent comprises composite probiotics powder and auxiliary materials. The composite probiotics powder is candida tropicalis powder, aspergillus niger agent, bacillus licheniformis powder and lactobacillus plantarum powder. The probiotics leavening agent can be used for cake dreg type feed raw material fermentation, can effectively degrade anti-nutritional factors in cake dregs, improves taste and protein quality of cake dreg feed, and accordingly effectively improve use ratio of the cake dregs in various livestock feed.

Description

technical field [0001] The invention belongs to the technical field of feed, and relates to a probiotic starter suitable for fermented cake feed raw materials and a preparation method thereof. Background technique [0002] The by-products of oil extraction from fat-rich legume seeds and oilseeds are collectively referred to as cake feed. The by-products after oil extraction are collectively referred to as cake feed. The cake-like by-products after oil extraction by pressing are called oil cakes, and the fragment-like or coarse powder-like by-products after oil extraction by extraction are called oil cakes. [0003] Oil cake and oil meal are the main vegetable protein feeds in my country, which are widely used and used in large quantities. Common cakes include soybean cake, cottonseed cake, rapeseed cake, peanut cake, sunflower cake, flax cake, and a small amount of walnut cake, safflower cake, sesame cake, castor cake meal, palm meal, etc. [0004] The common characteris...

Claims

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Application Information

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IPC IPC(8): A23K1/14
Inventor 闫轶洁宋维平贠桂玲李曼韩科厅
Owner BEIJING DABEINONG TECH GRP CO LTD
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