Probiotics leavening agent suitable for fermenting cake dreg type feed raw materials and preparation method of probiotics leavening agent
A feed raw material and starter technology, which is applied in animal feed, animal feed, application, etc., can solve the problems of mixed strains, single species, and poor effect, and achieve the effects of high number of viable bacteria, increased nutritional value, and improved taste
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[0025] Example 1 Preparation of Candida tropicalis powder
[0026] Candida tropicalis strain was purchased from China Food and Fermentation Industry Research Institute.
[0027] Seed medium: 1.0% yeast extract, 2.0% peptone, 2.0% glucose.
[0028] Culture conditions: 28°C, 180rpm, 24h.
[0029] Candida tropicalis culture medium: 1.0% yeast extract, 4.0% glucose, 1.0% peptone, 0.2% potassium dihydrogen phosphate, 0.03% magnesium sulfate.
[0030] Culture conditions: 28°C, 150rpm, 72h.
[0031] The obtained fermentation broth was centrifuged in a high-speed refrigerated centrifuge, and the centrifugal conditions: 6000 rpm, 4°C, 10 min. After centrifugation, the supernatant was discarded to obtain bacterial sludge. Mix 10% glycerol and 0.85% normal saline in a volume ratio of 1:1, dilute and stir the bacterium mud and mix well, then add 10% imported skimmed milk powder and mix well. Place it in an ultra-low temperature refrigerator at -80°C, then place it in a vacuum freeze dryer to dry ...
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[0032] Example 2 Preparation of Bacillus licheniformis powder
[0033] The strain of Bacillus licheniformis was purchased from China Food and Fermentation Industry Research Institute.
[0034] Seed medium: 0.5% beef extract, 1.0% peptone, 0.5% sodium chloride.
[0035] Culture conditions: 37°C, 180rpm, 24h.
[0036] Bacillus licheniformis culture medium: 3.0% brown sugar, 1.0% corn flour, 3.0% soybean meal, 0.25% urea, 0.15% sodium dihydrogen phosphate.
[0037] Culture conditions: 37°C, 180rpm, 24h.
[0038] The obtained fermentation broth was centrifuged with a high-speed refrigerated centrifuge under the conditions of the centrifugation: 5000rpm, 4°C, 10min. After centrifugation, the supernatant was discarded to obtain bacterial sludge. Stir the bacterium slurry with an aqueous solution of 5.0% sucrose, 1.0% sodium glutamate, and 1.0% ascorbic acid, and then add 50% imported skimmed milk powder. Place it in an ultra-low temperature refrigerator at -80°C, then place it in a vacuum f...
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[0039] Example 3 Preparation of Lactobacillus plantarum powder
[0040] The Lactobacillus plantarum strain was purchased from China Food and Fermentation Industry Research Institute.
[0041] The seed medium is a commercial MRS broth (a special medium for lactic acid bacteria).
[0042] Culture conditions: 37°C, 100rpm, 12h.
[0043] Lactobacillus plantarum fermentation medium: 3.5% peptone, 6.0% beef powder, 0.50% diammonium citrate, 3.5% sucrose, 1.0% yeast extract, 0.4% dipotassium hydrogen phosphate, 0.1% sodium acetate, 0.02% magnesium sulfate, 0.005% manganese sulfate, 0.05% Tween 80.
[0044] Culture conditions: 37°C, 50rpm, 12h.
[0045] The obtained fermentation broth was centrifuged with a high-speed refrigerated centrifuge under the conditions of the centrifugation: 5000rpm, 4°C, 10min. After centrifugation, the supernatant was discarded to obtain bacterial sludge. Stir the bacterium slurry with an aqueous solution of 5.0% sucrose, 1.0% sodium glutamate, and 1.0% ascorbic a...
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