Making method of lactic acid bacteria and compound enzyme solid state fermented corn germ meal

A corn germ meal, lactic acid bacteria fermentation technology, applied in the molding or processing of animal feed, animal feed, additional food elements, etc., can solve the problems of unbalanced amino acid composition, poor animal palatability, high crude fiber content, and increase feed intake. quantity and digestibility, harm reduction, and nutritional value enhancement

Inactive Publication Date: 2016-05-04
JILIN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The crude protein content of corn germ meal is between 20-27%, which is 2-3 times that of corn seed, but its lysine content is only 27% of soybean meal, and the amino acid composition is not balanced, and the crude fat content It is about 2%, which is close to the content of soybean meal, but the content of crude fiber is high, and due to the acidity of the taste, the palatability of animals is poor, so the use in the diet of growing and finishing pigs is very limited. The general addition amount is 5-10 %
At present, commonly used solutions include amino acid balance method, adding compound enzyme method, etc. The effects of different treatment methods vary greatly, and these methods fundamentally do not change the nutritional deficiencies of corn germ meal

Method used

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  • Making method of lactic acid bacteria and compound enzyme solid state fermented corn germ meal
  • Making method of lactic acid bacteria and compound enzyme solid state fermented corn germ meal
  • Making method of lactic acid bacteria and compound enzyme solid state fermented corn germ meal

Examples

Experimental program
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Effect test

Embodiment 1

[0023] With reference to the preparation method of the lactic acid bacteria of the present embodiment and the composite enzyme solid-state fermentation corn germ meal, it comprises the following concrete steps:

[0024] (1) Preparation of fermentation raw materials

[0025] The fermentation raw material - corn germ meal is crushed to 40 mesh (0.42mm) powder.

[0026] (2) Preparation of lactic acid bacteria liquid

[0027] Use a sterile pipette to draw a suitable liquid medium, drop it into the lactic acid bacteria freeze-dried powder preservation bottle, shake gently, so that the freeze-dried bacteria are suspended, draw all the bacterial suspension, and transplant it into the MRS solid medium. After culturing at ℃ for 36 hours, inoculate it on the MRS slant medium, inoculate it into the liquid medium after culturing on the slant surface for 36 hours, and culture it in shake flask for 12 hours, and the number of viable bacteria is 1.0×10 8 Inoculate the seed liquid into a 10...

Embodiment 2

[0033] With reference to the preparation method of the lactic acid bacteria of the present embodiment and the composite enzyme solid-state fermentation corn germ meal, it comprises the following concrete steps:

[0034] (1) Preparation of fermentation raw materials

[0035] The fermentation raw material - corn germ meal is crushed to 40 mesh (0.42mm) powder.

[0036] (2) Preparation of lactic acid bacteria liquid

[0037] Use a sterile pipette to draw a suitable liquid medium, drop it into the lactic acid bacteria freeze-dried powder preservation bottle, shake gently, so that the freeze-dried bacteria are suspended, draw all the bacterial suspension, and transplant it into the MRS solid medium. After culturing at ℃ for 42 hours, inoculate it on the MRS slant medium, inoculate it into the liquid medium after culturing it on the slant for 42 hours, and culture it in shake flask for 18 hours, and the number of viable bacteria is 3.0×10 8 Inoculate the seed liquid into a 100L se...

Embodiment 3

[0043] Referring to the preparation method of the lactic acid bacteria and compound enzyme solid-state fermentation corn germ meal of the present embodiment, it comprises the following specific steps:

[0044](1) Preparation of fermentation raw materials

[0045] The fermentation raw material - corn germ meal is crushed to 40 mesh (0.42mm) powder.

[0046] (2) Preparation of lactic acid bacteria liquid

[0047] Use a sterile pipette to draw a suitable liquid medium, drop it into the lactic acid bacteria freeze-dried powder preservation bottle, shake gently, so that the freeze-dried bacteria are suspended, draw all the bacterial suspension, and transplant it into the MRS solid medium. After culturing at ℃ for 48 hours, inoculate it on the MRS slant culture medium, inoculate it into the liquid medium after culturing it on the slant surface for 48 hours, and culture it in shake flask for 24 hours, and the number of viable bacteria is 2.0×10 8 Inoculate the seed liquid into a 10...

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Abstract

Belonging to the technical field of biological feed, the invention relates to a making method of lactic acid bacteria and compound enzyme solid state fermented corn germ meal. The method includes the steps of: crushing soybean meal into 40-mesh corn germ meal powder; introducing 5% of a lactic acid bacteria fermented seed solution with a viable count of 2.0*10<8>-2.0*10<9>cfu/ml into the corn germ meal powder, adding 0.5% of compound enzyme, adjusting the water content to 40% and the pH value to 5.0-7.0, mixing the substances evenly to obtain a fermentation base material; spreading the fermentation base material at a fermentation tank bottom at 30-45DEG C, carrying out anaerobic fermentation for 36-48h to obtain fermented corn germ meal, transferring the fermented corn germ meal into a fluidized bed to perform drying, and conducting cooling to room temperature so as to obtain a finished product. The corn germ meal is subjected co-fermentation by lactic acid bacteria and compound enzyme, the generated various enzymes, organic acids, amino acids and probiotics greatly improve the nutritional value of corn germ meal, and the protein content of the fermented corn germ meal is significantly increased, and the content of cellulose and other antinutritional factors is significantly decreased. As high quality protein feed, the lactic acid bacteria and compound enzyme solid state fermented corn germ meal can significantly increase the feed intake and digestibility of animals and enhance the antioxidant capacity of animal bodies.

Description

technical field [0001] The invention belongs to the technical field of biological feed, and in particular relates to a preparation method of solid-state fermented corn germ meal by lactic acid bacteria and compound enzymes. Background technique [0002] my country is the country with the most lack of protein feed resources in the world. The foreign dependence on protein feed has exceeded 70%. The annual import volume of soybean meal used for feed is as high as 50 million tons, and the import volume of fish meal is 1.2 million tons. The value is 150-180 billion yuan. Therefore, it is particularly important to develop and utilize unconventional protein feed resources. Corn germ meal is a by-product that uses corn as a raw material, soaking, crushing and separating the germ before producing starch or alcohol, and then extracting oil or oil. After the corn germ meal is pressed or extracted to extract the oil, except for the reduction of the fat content, the rest of the nutritio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K10/16A23K10/37A23K20/189A23K40/00
CPCY02P60/87
Inventor 魏炳栋陈群邱玉朗李林闫晓刚杨华明班志彬于维祁宏伟
Owner JILIN ACAD OF AGRI SCI
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