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Preparation method and application of feed being rich in contents of selenium and cordyceps militaris active substances

A fermented product, Lactobacillus plantarum technology, applied in animal feed, animal feed, bacteria used in food preparation, etc., can solve the problems of poor safety, low absorption and utilization rate, etc.

Inactive Publication Date: 2017-03-08
福建省安野金牛牧业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For selenium-deficient areas, by supplementing the inorganic selenium source—sodium selenite, its absorption and utilization rate is low and its safety is poor. Selenium supplementation should also be mainly rich in organic selenium, because organic selenium should be superior in terms of utilization rate. Inorganic selenium, and the selenium absorbed, transformed and enriched by microorganisms is a kind of selenium source with very high bioavailability

Method used

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  • Preparation method and application of feed being rich in contents of selenium and cordyceps militaris active substances
  • Preparation method and application of feed being rich in contents of selenium and cordyceps militaris active substances
  • Preparation method and application of feed being rich in contents of selenium and cordyceps militaris active substances

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0094] Embodiment 1, the preparation of basal medium (selenium-enriched Cordyceps militaris medium)

[0095] 1. Activation culture of Cordyceps militaris strain

[0096] The Cordyceps militaris strain was inoculated into the activation medium for activation culture, and cultured in the dark at 24°C for 7 days.

[0097] 2. Preparation of liquid seeds of Cordyceps militaris strain

[0098] 1. Suspend the spores of the Cordyceps militaris strain with sterile physiological saline to obtain a spore concentration of 1.8×10 7 individual / mL spore suspension.

[0099] 2, the spore suspension that step 1 obtains is inoculated in PDA liquid medium (the proportioning of spore suspension and PDA liquid medium is 5ml: 100ml), 24 ℃, 200r / min vibration culture 3 days, obtain liquid seed.

[0100] 3. Cultivation and collection of fruiting bodies of Cordyceps militaris

[0101] Inoculate 100ml of the liquid seeds obtained in step 2 into 1000g of Cordyceps militaris fruiting body wheat mediu...

Embodiment 2

[0109] Embodiment 2, the preparation of the seed liquid of many kinds of probiotics

[0110] 1. Preparation of Candida utilis S1204 strain seed solution

[0111] 1. Inoculate Candida utilis S1204 strain into PDA solid slant medium, and culture at 30°C for 20 hours.

[0112] 2. Suspend Candida utilis S1204 with 10ml sterile normal saline to obtain a bacterial concentration of 0.6-1.8×10 7 CFU / ml bacterial suspension.

[0113] 3. Inoculate 10ml of the bacterial suspension prepared in step 2 into 100g of seed strengthening medium, and incubate at 30°C for 16 hours to obtain strengthened seeds.

[0114] 4. Weigh 1 g of the strengthened seeds obtained in step 3, inoculate them into 100 mL of YPD liquid medium, and culture with shaking at 30° C. and 200 r / min for 16 hours to obtain Candida utilis S1204 seed liquid (the bacterial concentration in the seed liquid is 0.5 -1.5×10 8 CFU / mL).

[0115] 2. Preparation of Bacillus subtilis and Bacillus licheniformis seed liquid

[0116...

Embodiment 3

[0126] Embodiment 3, the preparation of fermented feed

[0127] Candida utilis S1204 seed liquid, Bacillus licheniformis seed liquid, Bacillus subtilis seed liquid, Lactobacillus casei seed liquid and Lactobacillus plantarum seed liquid prepared in Example 2 were used to carry out the following tests.

[0128] 1. The preparation process of fermented feed is divided into three test groups (both described with 100g solid medium as an example):

[0129] Experimental group A: the first stage: 10 mL of Candida utilis S1204 seed solution (1.0 × 10 9 CFU of Candida utilis), 10mL Bacillus licheniformis seed solution (10mL seed solution contains 1.4×10 9 CFU of Bacillus licheniformis) and 10mL Bacillus subtilis seed solution (10mL seed solution contains 1.8×10 9 CFU of Bacillus subtilis) was inoculated into 100g of solid fermentation medium A, cultivated at 33°C, stirred once every 6h from 0 to 24h, cultured statically from 24h to 36h, and added sterile water at 36h to increase the w...

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Abstract

The invention discloses a preparation method and an application of a feed being rich in the contents of selenium and cordyceps militaris active substances, and provides a method for preparing a mixed bacterium fermented material. The method comprises the following steps: (a1) inoculating a solid fermentation culture medium with candida utilis, bacillus licheniformis and bacillus subtilis for culturing; and (a2) after the step (a1) is completed, adding lactobacillus casei and lactobacillus plantarum to a culture system for fermentation culturing. The cordyceps militaris culture medium is used as a main raw material, and waste obtained after collecting selenium-enriched cordyceps militaris sporophore is sufficiently used. The products obtained by the method disclosed by the invention fill in the blank of the field of fermented feeds, a novel functional fermented feed integrating multiple physiological functions of cordyceps militaris active substances and selenium is developed, the technology is simple, feasible, green and environmentally friendly, and the method disclosed by the invention has high application values and wide prospects in the feed industry and breeding industry, and has great social benefits and economic benefits.

Description

technical field [0001] The invention relates to a preparation method and application of a feed rich in selenium and active substances of Cordyceps militaris. Background technique [0002] At present, the artificial cultivation technology of Cordyceps militaris has been quite mature, and its artificial culture medium mostly adopts wheat or rice culture medium, and the production has a considerable scale, and has obtained certain economic benefits. In the process of artificial cultivation of Cordyceps militaris, it is often the fruiting body of Cordyceps militaris with higher active ingredient such as cordycepin content that pays attention to the harvest, but less attention is paid to the medium (base) of Cordyceps militaris, which often results in a large number of pupae Cordyceps culture medium is discarded, sour and smelly, causing environmental pollution. Although the medium of Cordyceps militaris has been metabolized and utilized, its main components are still mostly sta...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K10/18A23K10/12A23K10/37A23K20/20
CPCA23V2400/125A23V2400/169Y02P60/87
Inventor 黄钦耿梁玲黄元君黄祥峰吴松刚
Owner 福建省安野金牛牧业有限公司
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