Production method of functional protein feed rich in four essential amino-acids

An essential amino acid and protein feed technology, which is applied in the directions of microorganism-based methods, biochemical equipment and methods, animal feed, etc., can solve the problems of increasing the cost of feeding, and it is difficult to increase the content of essential amino acids in fermented protein feeds, so as to reduce contamination and impurities. The probability of bacteria, shortening the solid-state fermentation time, and the effect of low energy consumption

Inactive Publication Date: 2015-08-19
河南双成生物科技有限公司 +1
View PDF12 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the amino acids currently used in feed are almost all pure products produced through biological fermentation, and then mixed and added to concentrated feed or full-price feed, which greatly increases the cost of feeding
[0003] Microorganisms such as bacillus, yeast, and lactic acid bacteria can be used to directly ferment agricultural and sideline products such as soybe...

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Production method of functional protein feed rich in four essential amino-acids
  • Production method of functional protein feed rich in four essential amino-acids
  • Production method of functional protein feed rich in four essential amino-acids

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] The functional protein feed production method rich in four essential amino acids of lysine, arginine, threonine and valine of the present invention comprises the steps:

[0029] The first step, liquid fermentation

[0030] Make a suspension of four types of Corynebacterium glutamicum (numbered CICC 20906, CICC 20191, CICC 20903 and CICC 20887) on beef extract peptone with 200ml sterile saline, and mix them at a ratio of 1:1:1:1 Afterwards, it is inoculated into the liquid fermentation medium and cultivated (60% liquid filling capacity), and the medium consists of: glucose 120g / L, (NH 4 ) 2 SO 4 30g / L, corn steep liquor 20g / L, KH 2 PO 4 1.5g / L, MgSO 4 ·7H 2 O0.5 g / L, biotin 8×10 -5 g / L, CaCO 3 30 g / L; pH 7.0, sterilized at 0.12 MPa for 20 min; culture conditions: tank pressure 0.1 Mpa, stirring speed 230 r / min, ventilation rate 1:0.25, culture temperature 30°C, fermentation time 48 h; YPD Candida parapsilosis (number CICC 1257) on the culture medium was made...

Embodiment 2

[0042] The functional protein feed production method rich in four essential amino acids of arginine, threonine, valine and tryptophan of the present invention comprises the following steps:

[0043] The first step, liquid fermentation

[0044] The preparation method of the mixed bacteria solution of four kinds of Corynebacterium glutamicum (numbered CICC 20191, CICC 20903, CICC 20887 and CICC 20192) and Candida parapsilosis (numbered CICC 1257) is the same as that in Example 1;

[0045] Take the slanted seeds of Bacillus natto (number CICC23413) and inoculate them in a 500ml eggplant-shaped bottle filled with peptone beef extract medium, and use 200ml of sterile saline to make a suspension of Bacillus natto first-grade solid seeds, and inoculate Fermentation culture in 2m3 fermenter (seed tank culture medium filling volume 60%), medium composition: 2.0% soybean flour, 1.5% corn flour, 0.4% magnesium sulfate, 0.05% manganese sulfate, 1.0% sodium chloride; Conditions: tank pres...

Embodiment 3

[0056] The functional protein feed production method rich in four essential amino acids of lysine, threonine, valine and tryptophan of the present invention comprises the following steps:

[0057] The first step, liquid fermentation:

[0058] Four kinds of Corynebacterium glutamicum (numbered as CICC 20906, CICC 20903, CICC 20887 and CICC 20192) and Candida parapsilosis mixed fermentation method are the same as in Example 1; the production method of Lactobacillus plantarum fermentation mash is the same as in Example 1 Identical; Bacillus natto fermentation mash production method is identical with embodiment 2.

[0059] The second step is to prepare the fermented mash:

[0060] Mix the fermented liquid prepared in the first step with 40 parts of Corynebacterium glutamicum and Trichosporium viscosus mixed bacteria fermented liquid, 20 parts of Lactobacillus plantarum fermented liquid, and 20 parts of Bacillus natto fermented liquid, and mix them evenly to obtain fermented mash...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a production method of a functional protein feed rich in four essential amino-acids. The production method comprises the following steps: firstly, carrying out liquid fermentation on corynebacterium glutamicum, candida parapsilosis, lactobacillus plantarum and bacillus natto; mixing fermented liquid rich in metabolites and living bacteria to prepared the mixed fermented liquid; then, mixing with one or more than two of bean pulp, cottonseed meal, rapeseed dregs and peanut meal at a certain ratio, and carrying out solid fermentation; and finally drying and packaging the product, so as to obtain the final product in which the crude protein content is greater than 50% and four essential amino-acids are abundant. According to the production method, the fermented liquid is directly mixed and fermented together with a solid material; the probability of contamination with infectious microbes is reduced; the energy consumption is low; no waste is discharged; the content of anti-nutritional factors in meal materials is lowered; the content of the essential amino-acids and protein in the raw materials is improved; the added economic value of agricultural and sideline products is improved; the essential amino acids in the finished feed are high in content; the adding amount of exogenous essential amino acids can be replaced; and the feed cost is reduced.

Description

technical field [0001] The invention relates to the technical field of animal feed production, in particular to a production method of functional protein feed rich in four essential amino acids. Background technique [0002] my country's aquaculture industry is well developed, and the main sources of a large amount of feed protein raw materials consumed each year are soybean meal and fish meal. Although the crude protein content of these two products is relatively high, there are problems such as uneven quality of fishmeal and increasing shortage of resources, while cake feed is relatively low in cost, but it belongs to vegetable protein, which contains a large amount of anti-nutritional factors, which affects animals. Digestion and absorption of feed. In order to solve this problem, in recent years there has been a technology of using mixed bacteria such as lactic acid bacteria, bacillus, saccharomyces and photosynthetic bacteria to ferment and produce feed protein raw ma...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A23K1/14A23K1/16C12N1/20C12N1/16C12R1/15C12R1/25C12R1/07C12R1/645
Inventor 曹胜炎李伟杨森宋安东谢慧李琰
Owner 河南双成生物科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap