Candida parapsilosis CCTCC M203011 (S)- carbonyl reduction enzyme gene sequence and amino acid composition

Abstract

The invention relates to gene order and amino acid component of (S)-carbonyl reductase of Candida parapsilosis CCTCC M203011, and the (S)-special carbonyl reductase gene can be used in separating recemic compound with biological method, which belongs to biological engineering sphere. The invention is to confirm (S)-special carbonyl reductase of Candida parapsilosis CCTCC M203011, its gene nucleotide sequence is SEQ ID NO:1, and its amino acid component is SEQ ID NO:2. By this gene, the recombination strain can be used in racemic chirality resolution and can arrange conditions for the heterogeneity, conversion approach and reaction mechanism of the microbial stereoselectivity oxidoreductase used in chirality resolution.

Description

technical field [0001] The gene sequence and amino acid composition of (S)-carbonyl reductase of Candida parapsilosis CCTCC M203011, the (S)-specific carbonyl reductase gene is used for biologically resolving racemic compounds, and belongs to the technical field of bioengineering . Background technique [0002] Optically pure phenylethylene glycol, namely (S)-phenylethylene glycol, which can also be referred to as (S)-PED, is not only an indispensable and important chiral additive in liquid crystal materials, but also has become an important chiral additive for the preparation of optically active materials. It is an important intermediate for medicines, pesticides and functional materials, and it is very meaningful to carry out research on the separation method of phenylethylene glycol. [0003] The preparation of optically pure chiral compounds by biological transformation has the advantages of mild reaction conditions, single product, high stereoselectivity, regioselectiv...

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Problems solved by technology

[0005] At present, some stereoselective oxidoreductase genes have been obtained by genetic engineering, and for the stereoselective oxidoreductase genes derived from Candida parapsilosis, only the dehydrogenation enzyme used to split 1,3-butanediol Enzymes CpSADH and polyketide reductases C1 and C2 for the asymmetric conversion of pantolactones were reported, but the homology of these enzymes is not high and there are differences between gene sequences