Cultivated silkworm microsporidian polar filament protein PTP3 gene and uses thereof

A technology of microsporidia and polar filament protein, applied in the direction of genetic engineering, plant genetic improvement, application, etc.

Inactive Publication Date: 2009-07-29
SOUTHWEST UNIVERSITY
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  • Abstract
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AI Technical Summary

Problems solved by technology

However, the current research on the polar tube protein of Microsporidia is limited t

Method used

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Embodiment Construction

[0202] Using Glimmer3.0 software, through the gene homology analysis of the genomes of N. silkworm and N. rabbit encephalitis, the CDS of the polar tube protein PTP3 gene sequence of N. silkworm was obtained; using Primer5.0 software, a Pair specific primers

[0203] PTP3-Primer-F5'-CGGGATCCCTACACATTATTTG-3'

[0204] PTP3-Primer-R5'-CGGTCGACGTGATGATGGGATTCC-3'

[0205] The amplified fragment was cloned into the pMD18-T vector, and the sequence determination was completed by Shanghai Yingjun, and the obtained full-length coding sequence of the polar filament protein PTP3; the homology comparison was completed by BLAST on the NCBI site, and the sequence analysis showed that it was a new protein gene.

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Abstract

The invention relates to a very important protein gene PTP3 related to propagation and infection of nosema bombycis nageli. A gene order CDS for encoding nosema bombycis nageli polar tube PTP3 is obtained according to 9-fold genome data of the nosema bombycis nageli and proven by a series of biotechnology clonings. The polar tube PTP3 obtained in the invention plays a very important role in studying a propagation and infection mechanism of microsporidian, thus playing a role in productive practice and prevention and treatment of the microsporidian disease. Experiments and analyses such as RNAi intervention and the like indicate that after the polar tube protein PTP3 is intervened, the microsporidian loses the capacity of infecting a host, therefore, the polar tube protein PTP3 of the microsporidian and potential regulator genes thereof become new factors for treating microsporidian infection, in addition, the method of modifying genes can be adopted to express the polar tube protein PTP3 in an abundant way, thus killing part of pests such as locust and the like.

Description

technical field [0001] The discovery relates to the field of biotechnology, in particular to an important protein PTP3 gene related to the reproduction and infection of Microsporidium silkworm. Background technique [0002] Microsporidia are intracellular obligate parasitic protozoa commonly distributed in nature. There are nearly 150 genera and more than 1,200 species. They have a very wide range of hosts, including vertebrates and invertebrates. They are economic insects, fish, rabbits, hair-producing Deadly pathogen of animals, rodents and primates. Nosema bombycis belongs to the phylum Protozoa, the subphylum Monofilamenta, and the class Microsporidia in taxonomic status. Microsporidium parasitizes the economic insect Bombyx mori and causes severe infectious microparticle disease, and because Microsporidium can be transmitted to the next generation through the embryo, it has been listed as the legal quarantine object of sericulture production in sericulture countries an...

Claims

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Application Information

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IPC IPC(8): C12N15/30C07K14/44A01N63/02A01P7/04
Inventor 周泽扬潘国庆许金山向仲怀
Owner SOUTHWEST UNIVERSITY
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