46results about How to "Address high mortality" patented technology

The invention discloses an interspecific hybridization and propagation method for deep sea scallops and chlamys farreri. The interspecific hybridization and propagation method comprises the following steps: a first step of breeding parent scallops: selecting deep sea scallops and chlamys farreri, separating the male and the female of the two scallops respectively, promoting maturity according to a normal parent scallop breeding method and using the mature scallops for cross experiment after synchronously maturing the mature scallops; a second step of laying eggs and performing hybridization: inducing spawning of the male and female groups of the two scallops respectively, fertilizing obtained eggs with conspecific or heterogeneous sperms respectively to obtain pure-bred self-bred progenies of the deep sea scallops and chlamys farreri as well as two hybrid fertilized eggs of female deep sea scallops and male chlamys farreri, female chlamys farreri and male deep sea scallops; hatching the obtained fertilized eggs at 13 to 14 DEG C, observing and measuring the proportion of D type larvas at regular times after the D type larvas appear until the maximum hatching rate is reached; a third step of cultivating scallops, wherein at a larval phase, the average shell height growth speed of the hybrid scallops is 6 to 6.3m per day, and the shell height, the shell length, the shell width and the weight are increased in a cultivating phase. According to the interspecific hybridization and propagation method disclosed by the invention, the deep sea scallops are effectively utilized through interspecific hybridization, so that the scallop breeding species are increased.

The invention discloses a method for establishing a systemic bone injury model and pathological mechanism analysis based on partial irradiation. Local irradiation of the knee joint is used to avoid high mortality and complicated pathological mechanisms of animals after whole-body irradiation; Irradiation damages intestines, kidneys, gonads and other organs closely related to bone metabolism; through single radiation damage to bone tissues and organs, the pathological mechanism of systemic radiation bone injury caused by local irradiation is effectively elucidated; it is helpful for the discovery of the pathological mechanism of radiation bone injury Early preventive intervention for clinical radiotherapy patients and as a model for new drug development and drug efficacy testing.

The invention discloses a method for efficiently cultivating ornamental lupine in a protected area. The inventive method adopts the method of cultivating ornamental lupine in a protected area. The seeds are germinated and treated at low temperature, seedlings are raised and planted in nutrient soil, and finished flowers are cultivated in combination with fertilization and light and temperature control. The main steps are: (1) Build a steel pipe greenhouse; (2) After the seeds are germinated at a constant temperature of 20°C±2°C, they are treated at a low temperature of 3°C±2°C for 45-60 days; (3) Use peat soil: chaff ash: leech Stone = 1:1:0.5 seedlings, select I level seedlings with more than 3 leaves; (4) Use vegetable garden soil: coal ash: river sand = 6:3:1 for planting; (5) Apply urea, cake fertilizer and compound fertilizer to promote growth , Spray KH2PO4 to promote flower bud differentiation, supplement light for 4 hours a day, and keep the shed temperature at 20-28°C; (6) Change pots for sale. Adopting the method of the invention to cultivate finished ornamental lupine flowers can significantly improve the germination rate of seeds, the preservation rate of seedlings, shorten the seedling cultivation cycle, promote flowering and improve the uniformity of flowering, and solve the technical problem of cultivating finished ornamental lupine flowers.

The invention relates to a cultivation method of deep-sea scallops. Atlantic deep-sea scallop breeding and breeding methods, including the introduction of deep-sea scallops and the artificial breeding and cultivation of deep-sea scallops. After 45-50 days, the broodstock reaches maturity and spawns. When most of the larvae have developed to D-shaped After the larvae, the larvae are selected and cultivated in a new pond with a screen box; the growth rate of the cultivated larvae is much higher than that of the larvae cultivated in the nursery in Quebec, Canada during the same period, and most of the larvae appear eyespots 16-17 days after fertilization. On the 18th day, the attachment base was cast. After 10 days of the attachment base, the attachment base was taken out and hung in the sea area to preserve the seedlings in the natural sea area to preserve the seedlings; the deep-sea scallops reached a commercial size with an average size of more than 8 cm and reached sexual maturity. The invention successfully introduces Atlantic deep-sea scallops into Chinese sea area for the first time, expands the species of scallops cultured, and promotes the construction of modern marine pastures.

The invention discloses a breeding method for scallop hybridization breeding. The breeding method is characterized by comprising the steps that firstly, gonad development synchronous breeding is carried out on selected chlamys farreri and Argopecten irradians; then, fully-developed female chlamys farreri in the chlamys farreri and the fully-developed Argopecten irradians are selected, after the female chlamys farreri finishes spawning, the hermaphroditic Argopectehs irradians is set for spermiation, and fertilization is carried out on sperms and eggs to form scallop hybridization fertilized eggs; incubation is carried out on the hybridization fertilized eggs, then larva selection is carried out, and larvas are bred in a larva breeding pond; finally the bred larvas are bred to scallop hybridization fry capable of being bred through settlement and metamorphosis. The hybridization scallop fry are bred from the female chlamys farreri and the Argopectehs irradias in a hybridization mode, and have the prominent advantages that in the breeding process, growing speed is high, resistance to diseases is high, the gonad is poor in fertility, the adductor muscle is large, the growing speed can be improved by more than 30 percent, the yield of primary products of the adductor muscle is improved by more than 40 percent, the obvious heterosis is achieved, and the sustainable development of scallop culture in China can be promoted.

The invention discloses a device and method for feeding poecilobdella manillensis manually. The device comprises a container and a pipe, wherein an opening is formed in the container, a heating and heat insulating device is arranged in the container, one end of the pipe is communicated with the bottom of the side face of the container through a control valve, and the other end of the pipe is detachably connected with a closed casing. The method for feeding the poecilobdella manillensis with the device comprises the steps of (1) placing blood in the container, and starting the heating and heat insulating device to enable the temperature of the blood to be kept between 36 DEG C and 39 DEG C; (2) opening the control valve and a selection valve to enable the casing to be filled with the blood, and feeding the hungry poecilobdella manillensis with the casing filled with the blood after it is ensured that no leakage exists through detection; (3) closing the control valve and the selection valve after the poecilobdella manillensis eats for 10-30 minutes, taking the used casing, cleaning the poecilobdella manillensis, and placing the poecilobdella manillensis back into a culture pond to be bred continuously. The device and method solve the problems of low feeding efficiency and waste of food and increase culture benefits.