46results about How to "Address high mortality" patented technology

The invention relates to a buffer solution for suspending animal or human cells and for dissolving biologically active molecules in order to introduce said biologically active molecules into the cells using an electric current and to a method for introducing biologically active molecules into animal or human cells using an electric current and a buffer solution. The inventive buffer solution has a buffering capacity of at least 20 mmol*I−1*pH−1 and an ionic strength of at least 200 mmol*I−1 during a change to the pH value from pH 7 to pH 8 and at a temperature of 25° C. The use of a buffer solution of this type in the corresponding method allows biologically active molecules to be introduced into animal and human cells with a high degree of transfection efficiency and at the same time a low cell mortality. Different cell types, in particular dormant and actively dividing cells of low activity, can be successfully transfected in said buffer solution.

The invention discloses a Muscovy duck formula pellet feed manufacturing method. The Muscovy duck formula pellet feed manufacturing method comprises the steps that cobs, poison sumac stem leaves and water spinach are saccharified, green plants comprising cucumbers and endive and cooked potatoes, cooked pumpkins, cooked Chinese yam, cooked pepper, cooked bone meal and the like are added, and after the mixing technology, the pellet manufacturing technology, the drying technology and the like are adopted, nutritive fitness type Muscovy duck daily feed is manufactured, wherein the feed is rich in vegetable protein, multivitamin, pectin and the like and capable of whetting appetite, promoting growth and achieving the functions of clearing heat and toxin and relaxing the bowel. The finished feed is rich in soft fiber, compact in pellet, good in taste, high in conversion efficiency and capable of preventing and treating staphylococcus aureus and dysentery which often attack Muscovy dusks.

The invention discloses an interspecific hybridization and propagation method for deep sea scallops and chlamys farreri. The interspecific hybridization and propagation method comprises the following steps: a first step of breeding parent scallops: selecting deep sea scallops and chlamys farreri, separating the male and the female of the two scallops respectively, promoting maturity according to a normal parent scallop breeding method and using the mature scallops for cross experiment after synchronously maturing the mature scallops; a second step of laying eggs and performing hybridization: inducing spawning of the male and female groups of the two scallops respectively, fertilizing obtained eggs with conspecific or heterogeneous sperms respectively to obtain pure-bred self-bred progenies of the deep sea scallops and chlamys farreri as well as two hybrid fertilized eggs of female deep sea scallops and male chlamys farreri, female chlamys farreri and male deep sea scallops; hatching the obtained fertilized eggs at 13 to 14 DEG C, observing and measuring the proportion of D type larvas at regular times after the D type larvas appear until the maximum hatching rate is reached; a third step of cultivating scallops, wherein at a larval phase, the average shell height growth speed of the hybrid scallops is 6 to 6.3m per day, and the shell height, the shell length, the shell width and the weight are increased in a cultivating phase. According to the interspecific hybridization and propagation method disclosed by the invention, the deep sea scallops are effectively utilized through interspecific hybridization, so that the scallop breeding species are increased.

The invention relates to an aquaculture method of deep sea scallops, in particular to a breeding and propagation method for Atlantic deep sea scallops. The method comprises the steps that introduction, artificial breeding and aquaculture of the deep sea scallops are carried out, parent scallops are mature after 45-50 days maturity promoting and lay eggs, and after most larvae grow into D-shaped larvae, a bolting cloth net cage is used for selecting optimal larvae to a new pond for cultivation; the growth rate of the cultured larvae is far higher than that of the larvae cultured in a nursery ground in Quebec of Canada at the same time, eyespots are generated on most larvae 16-17 days after the larvae are fertilized, adhering substances are arranged at the 18th day, after the adhering substances are arranged for 10 days, the adhering substances are taken out and hung in natural sea ears for larva protection; the deep sea scallops meet commercial specification when the cultivated average specification reaches 8 centimeters, and the deep sea scallops achieve sexual maturity. According to the method, the Atlantic deep sea scallops are introduced into China sea areas for the first time, the scallop culture species are expanded, and construction of modern ocean ranches is promoted.

The invention discloses an ectopic paddy field-penaeus monodon ecological breeding system which sequentially comprises a water purification unit, a primary purification paddy field, a secondary purification paddy field, a plurality of high-position shrimp breeding tanks and / or water storage tanks and a plurality of penaeus monodon greenhouses according to a water flow direction. A water inlet ditch is arranged on one side of each penaeus monodon greenhouse and communicated with the corresponding high-position shrimp breeding tank and / or water storage tank, and a drainage ditch is arranged on the other side of each penaeus monodon greenhouse and communicated with the primary purification paddy field. The organic paddy fields are separated from the high-position shrimp breeding tanks and thepenaeus monodon greenhouses, an ectopic paddy field-penaeus monodon ecological breeding mode is established, the problem of high death rate of intensive breeding of penaeus monodons is solved, penaeus monodon production and quality are improved, and both production and income are increased.

The invention discloses a Barbus capito fry feed relating to a fish feed. The invention solves the problems of high breeding cost of Barbus capito fry fed by other fish feeds, slow Barbus capito fry weight increment and high death rate. The Barbus capito fry feed is composed of wheat short, bean pulp, fish meal, Subaokang, food meal, cottonseed meal, corn albumen powder, soybean oil, fish oil, multi-vitamin for fishes, composite trace element, choline, MgSO4.7H2O, Yiduo enzyme P8901 and sodium carboxymethylcellulose. The Barbus capito fry feed of the invention has low cost, satisfies the requirement on feeding Barbus capito fry feed, solves the problem existing in Barbus capito fry feed production, avoids the problems of high cost of other fish feeds, slow weight increment and high death rate, improves the growth speed and survival rate of the Barbus capito fry and accelerates the healthy growth of the Barbus capito fry.

The invention relates to application of umbilical cord Wharton jelly originated mesenchymal stem cells used as cellular transplantation materials for treating heart failures caused by coronary heart diseases and dilated cardiomyopathies, wherein the heart failures include chronic ischemic heart failures caused by myocardial infarction, final-stage ischemic heart failures, and the heart failures caused by secondary cardiomyopathies after primary dilated cardiomyopathies and myocarditis.

The present invention provides a non-human animal model of myocardial infarction, which can be created by a less invasive method, shows an extremely low mortality during and after surgical induction of myocardial infarction, ensures the blood reflow after necrosis of cardiac muscles caused by blocking blood flow, and therefore, is usable in topical therapy for myocardial infarction and studies on regenerative medicine. The present invention also provides a method for creating the non-human animal model comprising administering a temporary embolic agent to a branch blood vessel of a heart coronary artery to block blood flow in the branch blood vessel; and after a definite period of time after said vascular branch blockage, administering a temporary embolic agent to the target coronary artery which is intended to induce myocardial infarction to block blood flow in the target coronary artery.

The invention provides a new ostrea rivularis species breeding method. Firstly, ostrea rivularis and crassostrea gigas parents are selected, and oyster parent ripening promotion breeding is conducted; secondly, ostrea rivularis and crassostrea gigas are manually dissected, female ostrea rivularis and male crassostrea gigas are selected in a microscopic examination mode, and eggs of ostrea rivularis and sperms of crassostrea gigas are taken out for fertilization to form hybrid fertilized ovums of ostrea rivularis; thirdly, after incubation of the hybrid fertilized ovums, larvae are selected and bred in a larva breeding pond; finally, settlement and metamorphosis are conducted on the bred larvae, and a first generation of hybrid fry of ostrea rivularis for breeding is bred, and through artificial breeding, new ostrea rivularis species which is high in disease resistance and growing speed and good in meat quality is screened out to be bred.

The invention discloses a breeding method for scallop hybridization breeding. The breeding method is characterized by comprising the steps that firstly, gonad development synchronous breeding is carried out on selected chlamys farreri and Argopecten irradians; then, fully-developed female chlamys farreri in the chlamys farreri and the fully-developed Argopecten irradians are selected, after the female chlamys farreri finishes spawning, the hermaphroditic Argopectehs irradians is set for spermiation, and fertilization is carried out on sperms and eggs to form scallop hybridization fertilized eggs; incubation is carried out on the hybridization fertilized eggs, then larva selection is carried out, and larvas are bred in a larva breeding pond; finally the bred larvas are bred to scallop hybridization fry capable of being bred through settlement and metamorphosis. The hybridization scallop fry are bred from the female chlamys farreri and the Argopectehs irradias in a hybridization mode, and have the prominent advantages that in the breeding process, growing speed is high, resistance to diseases is high, the gonad is poor in fertility, the adductor muscle is large, the growing speed can be improved by more than 30 percent, the yield of primary products of the adductor muscle is improved by more than 40 percent, the obvious heterosis is achieved, and the sustainable development of scallop culture in China can be promoted.

The invention discloses a penaeus monodon greenhouse high-efficiency cycle breeding method which includes the steps of breeding greenhouse building, preparation before penaeus monodon stocking, shrimpseed stocking, feeding management, water quality management and the like. Compound microbial agents are prepared, advantageous microbial communities are formed in water, and water quality pollution is reduced. The method is simple and applicable in technology, low in cost input and high in operability, the breeding risk of penaeus monodons is reduced, and diseases are decreased. Recycling of water in a greenhouse is improved, water eutrophication caused by massive application of feed is greatly improved, water change frequency is decreased, the appetite of the penaeus monodons can be improved, and the digestion and use ratio of the feed is increased.

The invention discloses an amphibians crossing channel ecological module which comprises a channel body passage, a channel bottom climbing plate, a light reflection and heat insulation plate, a support and a guide protection plate. The front end and the back end of the channel body passage are provided with fixing devices and are arranged on the two sides of a channel; the channel body passage is in a slot shape, and a water absorbing material is contained in the slot and is made of cotton, fiber, silk floss, felt and / or hay; the light reflection and heat insulation plate is in a hopper shape, a water storage tank is arranged below the light reflection and heat insulation plate, and the hopper-shaped light reflection and heat insulation plate is used for collecting rainwater and supplanting the water storage tank for water, the water storage tank drips water into the water absorbing material in the slot of the channel body passage through water outlet pipes, and the water absorbing material keeps wet; and soil bases are arranged on the two sides of the channel body passage, a hidden rest place is formed by dead branches placed at the edges and stone riprap, and amphibians are induced to pass through the channel.

The invention provides a healthy aquatic microcapsule biological feed which is prepared by biochemically-treated raw materials, namely, composite high protein power, algae single-cell extractive, microbial protein yeast, detoxification and fermentation potato powder, a micro-ecology nutrient solution and a food calling coating agent by a curing material production device, a micro-ecology nutrient solution emulsifying mixing pelletizing device and a coating layer coating microcapsule production device. The quality of the feed can be improved, the production cost can be reduced, and the safety probability of the food can be improved since the chemical antibiotics additive is replaced by the biological product; the nutrition ingredients of the raw materials of the feed can be protected due to a microcapsule synthesis production technology; the gustatory difficulty of seedling feeding can be solved due to the food calling coating agent; and the feed is persistent in water due to the microcapsule, so that the pollution of the feed residue to the water quality can be reduced.

The invention relates to a hairtail domesticating method, and belongs to the technical field of aquaculture and transportation. The domesticating method comprises the steps of culture equipment preparation, hairtail putting, bait casting, daily management and screening. According to biological characteristics of hairtail, by selecting proper culture equipment and biological bait and performing daily management, the problem that the hairtail indoor culture death rate is high is effectively solved, meanwhile domesticating operation is easy and feasible, popularization is easy, the hairtail domesticating gap is filled, and the important breakthrough that hairtail is migrated from the sea to the artificial feeding environment is achieved.

The invention discloses a traditional Chinese medicine preparation for treating small water turtle saprolegniasis and a preparation method of the traditional Chinese medicine preparation, and belongs to the technical field of turtle feed additive processing. The traditional Chinese medicine preparation comprises the following raw materials: garlic, tangerine peel, gallnut, scutellaria baicalensis, radix sophorae flavescentis, ginger, ephedra, polygonum hydropiper, agastache rugosus, calamus, fructus cnidii, Chinese lobelia, rhizoma smilacis glabrae, radix angelicae, sargentgloryvine stems, oldenlandia diffusa, verbena officinalis, gentian violet, honeysuckle and wild chrysanthemum flower. The traditional Chinese medicine preparation is prepared by drying, crushing, screening, mixing and by sterilizing. The traditional Chinese medicine preparation provided by the invention integrates the effects of a plurality of traditional Chinese medicines, and has the functions of antisepsis and anti-inflammation, heat-clearing and detoxicating, body immunity improvement and the like; the medicinal effects of the traditional Chinese medicine preparation can be well absorbed by small water turtles, the death rate of the small water turtles suffering from the rotten-skin disease can be effectively reduced, and the breeding risk can be reduced; the preparation method is simple to operate, low in cost and beneficial for popularization and application.

The invention relates to a making method of leech polypeptide medicinal liquor having effect of preventing and treating cardiovascular and cerebrovascular diseases. The making method comprises the following steps of crushing dried spina date seeds, dried radix angelica sinensis, dried ligusticum wallichii and dried longan, performing soaking with virgin pulp liquor, adding leech polypeptide extracting liquid, and performing compounding to obtain the leech polypeptide medicinal liquor. The leech polypeptide medicinal liquor prepared by the method has the effects of improving sleep quality, relieving dizziness and headache symptoms, strengthening capacity of tissue and organs for blood supply and oxygen supply, accelerating blood flow, activating blood circulation, reducing blood consistency, conditioning blood fat, reducing cholesterol and fat content in plasma, strongly eliminating garbage toxins in the blood, enabling thrombosis to dissolve, softening blood vessels, improving immunity, eliminating atherosclerotic plaque, preventing angiorrhexis and the like, and can effectively prevent and treat cardiovascular and cerebrovascular diseases.

The invention belongs to the technical field of animal raising and discloses a manual semen collection and injection method for geese. According to the method, semen of the male geese is manually collected and manually injected into the female geese, the number of the raised male geese is decreased, the elimination time of the male geese is prolonged, the reproductive performance of the female geese is improved, and the utilization age limit of the female geese is higher. Therefore, the raising cost of the geese is reduced, and the economic benefits of goose raising are increased.

The invention relates to a Tibetan chicken breeding method, and aims at providing a disinfectant scheme to overcome the defects in the Tibetan chicken breeding process. The disinfectant is formed by mixing 1-5 percent of magnesium carbonate solution, 1-5 percent of disodium hydrogen phosphate solution and 3-10 percent of saponated cresol solution according to the volume ratio 1: 1: 2. Disinfection is carried out 2-4 times each week in summer, and disinfection is carried out 1-3 times each week in winter. Disinfectant for a henhouse comprises 0.02-0.08 percent of potassium permanganate solution and 0.01-0.05 percent of peracetic acid solution, and is sprayed to disinfect the henhouse every other day. The Tibetan chicken breeding method is simple and easy to popularize and apply, the problem of high death rate in a traditional breeding process is solved through the henhouse and stocking combined breeding method and the disinfectants with different prescriptions, and the death rate is reduced by 14.1 percent.

The invention discloses a fast breeding method of African lily, and relates to the field of African lily breeding. The method comprises the steps of 1, seed selection, wherein mature and full seeds from places of origin are selected; 2, primary culture, wherein the seeds are dibbled in an agar culture medium; 3, sieving, wherein complete and sprouted seeds are selected, radicles of the seeds are but off, and other parts serve as explants; 4, secondary culture, wherein the explants are cleaned up and placed into a multiplying culture medium; 5, cutting, wherein sprouts are cleaned up, cut intosingle plants and placed into a cultivation culture medium; 6, transplanting, wherein the single plants are placed into a basin body containing a medium, and fertilizers are applied regularly. According to the fast breeding method of the African lily, the problem of a low germination rate when the African lily is planted in a soilless culture mode is solved; the problems of long breeding consumingtime, a low breeding coefficient and a high death rate when the African lily is planted are effectively solved through scientific control and management according to growth characteristics of all periods of the African lily, and the requirements of scaled development and marketization of the African lily are finally met.

The invention discloses an interspecific hybridization and propagation method for deep sea scallops and chlamys farreri. The interspecific hybridization and propagation method comprises the following steps: a first step of breeding parent scallops: selecting deep sea scallops and chlamys farreri, separating the male and the female of the two scallops respectively, promoting maturity according to a normal parent scallop breeding method and using the mature scallops for cross experiment after synchronously maturing the mature scallops; a second step of laying eggs and performing hybridization: inducing spawning of the male and female groups of the two scallops respectively, fertilizing obtained eggs with conspecific or heterogeneous sperms respectively to obtain pure-bred self-bred progenies of the deep sea scallops and chlamys farreri as well as two hybrid fertilized eggs of female deep sea scallops and male chlamys farreri, female chlamys farreri and male deep sea scallops; hatching the obtained fertilized eggs at 13 to 14 DEG C, observing and measuring the proportion of D type larvas at regular times after the D type larvas appear until the maximum hatching rate is reached; a third step of cultivating scallops, wherein at a larval phase, the average shell height growth speed of the hybrid scallops is 6 to 6.3m per day, and the shell height, the shell length, the shell width and the weight are increased in a cultivating phase. According to the interspecific hybridization and propagation method disclosed by the invention, the deep sea scallops are effectively utilized through interspecific hybridization, so that the scallop breeding species are increased.

The invention discloses a method for establishing a systemic bone injury model and pathological mechanism analysis based on partial irradiation. Local irradiation of the knee joint is used to avoid high mortality and complicated pathological mechanisms of animals after whole-body irradiation; Irradiation damages intestines, kidneys, gonads and other organs closely related to bone metabolism; through single radiation damage to bone tissues and organs, the pathological mechanism of systemic radiation bone injury caused by local irradiation is effectively elucidated; it is helpful for the discovery of the pathological mechanism of radiation bone injury Early preventive intervention for clinical radiotherapy patients and as a model for new drug development and drug efficacy testing.

The invention discloses a method for transforming a soda saline-alkali soil waste fishpond into a typha wetland, and relates to a transformation method of the soda saline-alkali soil waste fishpond. The method solves the problems of low seedling germination rate, high death rate and low population density in the typha transplanting process of the soda saline-alkali soil fishpond. The method comprises the following steps of 1, desalting the waste fishpond and carrying out micro-landform modification; 2, collecting and treating typha rhizomes; 3, transplanting the typha rhizomes; and 4, performing ecological water supplementing in the growth period. The method is high in operability and clear in target, water resource waste caused by blind water supplementation in the middle growth stage oftypha is avoided by establishing reasonable typha ecological water supplementation measures, and typha seedlings are high in germination speed, germination rate and population density, and low in death rate.

The invention relates to a cultivation method of deep-sea scallops. Atlantic deep-sea scallop breeding and breeding methods, including the introduction of deep-sea scallops and the artificial breeding and cultivation of deep-sea scallops. After 45-50 days, the broodstock reaches maturity and spawns. When most of the larvae have developed to D-shaped After the larvae, the larvae are selected and cultivated in a new pond with a screen box; the growth rate of the cultivated larvae is much higher than that of the larvae cultivated in the nursery in Quebec, Canada during the same period, and most of the larvae appear eyespots 16-17 days after fertilization. On the 18th day, the attachment base was cast. After 10 days of the attachment base, the attachment base was taken out and hung in the sea area to preserve the seedlings in the natural sea area to preserve the seedlings; the deep-sea scallops reached a commercial size with an average size of more than 8 cm and reached sexual maturity. The invention successfully introduces Atlantic deep-sea scallops into Chinese sea area for the first time, expands the species of scallops cultured, and promotes the construction of modern marine pastures.

The invention discloses a breeding method for scallop hybridization breeding. The breeding method is characterized by comprising the steps that firstly, gonad development synchronous breeding is carried out on selected chlamys farreri and Argopecten irradians; then, fully-developed female chlamys farreri in the chlamys farreri and the fully-developed Argopecten irradians are selected, after the female chlamys farreri finishes spawning, the hermaphroditic Argopectehs irradians is set for spermiation, and fertilization is carried out on sperms and eggs to form scallop hybridization fertilized eggs; incubation is carried out on the hybridization fertilized eggs, then larva selection is carried out, and larvas are bred in a larva breeding pond; finally the bred larvas are bred to scallop hybridization fry capable of being bred through settlement and metamorphosis. The hybridization scallop fry are bred from the female chlamys farreri and the Argopectehs irradias in a hybridization mode, and have the prominent advantages that in the breeding process, growing speed is high, resistance to diseases is high, the gonad is poor in fertility, the adductor muscle is large, the growing speed can be improved by more than 30 percent, the yield of primary products of the adductor muscle is improved by more than 40 percent, the obvious heterosis is achieved, and the sustainable development of scallop culture in China can be promoted.

The invention discloses mandarin fish artificial compound feed produced by fermented silkworm pupae, and particularly relates to the field of mandarin fish culture feed. A main material of the mandarin fish artificial compound feed is prepared from the following raw materials in parts by weight: 20-30 parts of the silkworm pupae, 30-40 parts of soybean oil cakes, 10-12 parts of potato flour, radix, 4-6 parts of radix astragali, 2-4 parts of radix codonopsis, 4-6 parts of flos lonicerae, 4-6 parts of a multi-element mineral salt, 4-6 parts of a vitamin mixture, 1-4 parts of a growth promoting agent, 2-5 parts of an emulsifier and 2-5 parts of an adhesive. According to the mandarin fish artificial compound feed, through microbial fermentation of the silkworm pupae, the odor of the silkworm pupae can be directly eliminated, chitin and allergens of pupariums are degraded, some secondary metabolites with functions can further be produced, certain living bacteria are kept, and the nutritionand digestibility utilization rate of the silkworm pupae after fermentation is obviously increased; and the prepared mandarin fish compound feed can not only promote the growth and health of mandarinfish, but also improve the quality of the mandarin fish, fish meal is saved, and economic benefits are increased.

The beer yeast sterilizing method features that rationing adding pump is set in the beer yeast recovering or circulating pipeline and rationing adding tank with compounded phosphoric acid solution orbactericide, so that phosphoric acid solution or bactericide is added simultaneously in certain amount to the beer yeast to lower its pH value slowly to reach 2.2-2.3 ultimately for killing bacteria. The said method can avoid high death rate and senility of yeast after acid treatment while obtaining bactericidal rate as high as 99 %, and this can ensure beer yeast purity and high beer product quality.

The invention discloses an application of cantharidin in preparation of a drug for preventing and treating osteosarcoma growth and metastases as an miR-214-3p / Wnt / beta-Catenin signal pathway inhibitor. The invention provides a novel method which can be used for preventing and treating osteosarcoma occurrence, development, metastases and drug resistance. The problems of a high death rate and shortsurvival time of a patient with osteosarcoma can be effectively solved.

A seedling planting method, the method steps are as follows: site preparation, planting, seedling support winding and watering. By preparing the soil in advance, the planting area between each seedling can be effectively controlled, and the growth inhibition between the seedlings caused by excessive planting density can be prevented in the later stage. At the same time, the addition of the fungicide can effectively kill the bacteria in the growth process of the seedlings, and prevent the high mortality rate of the seedlings due to infection during the growth process. The mixture of ammonium nitrate, potassium dihydrogen phosphate and water can supplement the nutrients needed by plants during the growth process and ensure sufficient nutrition for seedlings. The planting method of the invention can greatly improve the survival rate of seedling planting, ensuring that the survival rate of seedlings is above 95%.

The invention discloses a device and method for feeding poecilobdella manillensis manually. The device comprises a container and a pipe, wherein an opening is formed in the container, a heating and heat insulating device is arranged in the container, one end of the pipe is communicated with the bottom of the side face of the container through a control valve, and the other end of the pipe is detachably connected with a closed casing. The method for feeding the poecilobdella manillensis with the device comprises the steps of (1) placing blood in the container, and starting the heating and heat insulating device to enable the temperature of the blood to be kept between 36 DEG C and 39 DEG C; (2) opening the control valve and a selection valve to enable the casing to be filled with the blood, and feeding the hungry poecilobdella manillensis with the casing filled with the blood after it is ensured that no leakage exists through detection; (3) closing the control valve and the selection valve after the poecilobdella manillensis eats for 10-30 minutes, taking the used casing, cleaning the poecilobdella manillensis, and placing the poecilobdella manillensis back into a culture pond to be bred continuously. The device and method solve the problems of low feeding efficiency and waste of food and increase culture benefits.