The invention discloses a local irradiation fixation device for nude mice, comprising a base, a first slide rail, a first slide block, a shielding plate, a second slide rail, a second slide block, a third slide block, a third slide rail, a fourth slide Slider, fixing plate, placement slot, soft pad, screw, pressing plate, this device for local irradiation of nude mice has ingenious structure and powerful functions. By using this device, it can not only effectively fix the limbs of experimental nude mice, so as to avoid The escape of nude mice improves the convenience of the experimental operation. In addition, according to the needs, the partial radiation protection of the nude mice can be realized through simple operations, thereby greatly improving the effectiveness of the experimental research.