Method for establishing cognitive function injury rat animal model

An animal model and cognitive function technology, applied in the field of radiation protection, can solve problems such as cognitive function impairment, and achieve the effects of good reproducibility, low cost, and simple operation

Pending Publication Date: 2019-02-26
CHINA INST FOR RADIATION PROTECTION
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, none of the above modeling methods can ...

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  • Method for establishing cognitive function injury rat animal model
  • Method for establishing cognitive function injury rat animal model
  • Method for establishing cognitive function injury rat animal model

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1: Animal Radiation Exposure

[0022] SD rats, SPF grade, male, 4-5 weeks old, provided by the Experimental Animal Center of the Academy of Military Medical Sciences of the Chinese People's Liberation Army, production license number: SCXK-(military) 2012-0004, certificate number: 0035516.

[0023] After 6 days of quarantine, the animals were randomly divided into control group (Control, C), 10Gy simple irradiation group (10GyIR), 20Gy simple irradiation group (20Gy IR), and 30Gy simple irradiation group (30Gy IR) according to body weight, with 5 animals in each group .

[0024] (1) Control group: each animal did not receive radiation exposure, and was given purified water by gavage, with a volume of 20ml / kg;

[0025] (2) 10Gy simple irradiation group: each animal received a single 10Gy radiation exposure;

[0026] (3) 20Gy simple irradiation group: each animal received a single 20Gy radiation exposure;

[0027] (4) 30Gy simple irradiation group: each animal r...

Embodiment 2

[0030] Embodiment 2: Detection test of animals after radiation exposure

[0031] 1. Test equipment

[0032] Morris water maze (Institute of Materia Medica, Chinese Academy of Medical Sciences), medical electron linear accelerator synergy (Elekta Limited), paraffin embedding machine Histocentre 3 (Shandon), paraffin slicer Finesse 325 (Shandon), automatic tissue dehydrator Excelsior ES (Shandon ), fluorescence microscopy imaging system BX53 (Olympus).

[0033] 2. Morris water maze test

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Abstract

The invention belongs to the technical field of radiation protection and relates to a method for establishing a cognitive function injury rat animal model. The method comprises the following steps of:after a rat is anesthetized, carrying out single head local irradiation by using rays so as to form the cognitive function injury animal model. By utilizing the method, the established rat model canbetter simulate the cognitive function injury of a human body caused by radiation.

Description

technical field [0001] The invention belongs to the technical field of radiation protection, and relates to a method for establishing a rat animal model of cognitive function impairment. Background technique [0002] At present, radiotherapy is an important method for the treatment of primary and metastatic head and neck tumors. Due to the maturity and promotion of modern radiation techniques such as three-dimensional conformal, intensity-modulated conformal and γ (X) knife, the survival rate and survival time of head and neck tumor radiotherapy patients have increased. However, while radiation therapy damages tumor cells, it will also inevitably damage normal tissues within the irradiated area, thereby causing radiation damage. Typically, 90% of patients with a survival period of more than 6 months will develop cognitive dysfunction, of which 2%-5% of long-term survivors will go on to develop dementia. [0003] Cognitive dysfunction can be manifested as abnormalities in l...

Claims

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Application Information

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IPC IPC(8): A61D7/00
CPCA61D7/00
Inventor 秦秀军刘梦雅王新钢尹晶晶袁慧高洁岳娟刘欢王永丽李建国安全李梅
Owner CHINA INST FOR RADIATION PROTECTION
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