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Application of umbilical cord Wharton jelly originated mesenchymal stem cells in heart failure cellular transplantation therapy

A technology of mesenchymal stem cells and heart failure, applied in the direction of medical raw materials derived from mammals, cardiovascular system diseases, microorganisms, etc., can solve the problems of reduced para-autocrine function and failure to achieve biological effects, so as to improve prognosis and solve The effect of high mortality

Active Publication Date: 2010-04-07
PLA NAVY GENERAL HOSIPTAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The technical problem to be solved by the present invention is to apply the mesenchymal stem cells derived from umbilical cord Huatong jelly to patients with chronic heart failure caused by coronary heart disease and dilated cardiomyopathy as a material for cell transplantation to improve Improve the quality of life of these patients with chronic heart failure and reduce mortality; at the same time overcome the defects of coronary heart disease and elderly patients with autologous stem cell differentiation and proliferation and reduced para-autocrine function that cannot achieve effective biological effects

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Example 1 Preparation of UW-MSCs transplantation materials—Using fresh UW-MSCs

[0018] Fresh umbilical cord Huatong jelly mesenchymal stem cells that have just been subcultured for 2-4 generations were digested with 0.05-0.25% trypsin for 1-5 minutes, serum was neutralized, washed 3 times with balanced salt solution, and the centrifugal force of the centrifuge was controlled. In 100-1000g, it should be resuspended in 0.9% sodium chloride injection containing 0-100 units of heparin / ml for use.

Embodiment 2

[0019] Example 2 Preparation of UW-MSCs transplantation material - application of cryopreserved UW-MSCs spares

[0020] Take out the frozen 2-4 generation umbilical cord Huatong jelly mesenchymal stem cells from the cryogenic container, quickly put them into a 35-38°C incubator and water bath, dissolve within 2 minutes, and resuspend the cells with complete medium solution. After culturing for 2-4 days for cell fusion, re-digest and wash the cells, use heparin sodium chloride injection with a concentration of "1000 units of heparin: 100 ml of 0.9% sodium chloride solution" to make a cell concentration of 1-2×10 6 / ml of cell suspension, usually packed in 10ml tubes.

Embodiment 3

[0021] Example 3 Surgical method of transvenous transplantation of UW-MSCs

[0022] Will 2×10 7 The UW-MSCs suspension was diluted in 20ml heparin saline (heparin 200 units + normal saline 20ml) and injected rapidly from the vein.

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PUM

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Abstract

The invention relates to application of umbilical cord Wharton jelly originated mesenchymal stem cells used as cellular transplantation materials for treating heart failures caused by coronary heart diseases and dilated cardiomyopathies, wherein the heart failures include chronic ischemic heart failures caused by myocardial infarction, final-stage ischemic heart failures, and the heart failures caused by secondary cardiomyopathies after primary dilated cardiomyopathies and myocarditis.

Description

technical field [0001] The invention relates to the use of biological materials in the medical field, in particular to the application of mesenchymal stem cells prepared from human umbilical cord Huatong jelly in the preparation of heart failure cell transplantation materials caused by coronary heart disease and dilated cardiomyopathy. Background technique [0002] About 17 million people die from cardiovascular diseases worldwide every year. Although the mortality rate of acute myocardial infarction has decreased significantly with the improvement of drug treatment, interventional technology and surgical operation, evidence-based medicine has found that even if the patients with acute myocardial infarction who successfully received early revascularization therapy still have more than 30% of patients develop ischemic heart failure and even develop end-stage ischemic heart failure. End-stage ischemic heart failure refers to: ① a history of myocardial infarction; ② severe cli...

Claims

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Application Information

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IPC IPC(8): A61K35/12A61P9/04C12N5/08A61K35/51
Inventor 高连如张宁坤陈宇杨晔丁青艾朱智明曹毅
Owner PLA NAVY GENERAL HOSIPTAL
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